Publikace

@article{nokey,

title = {Mutations of the brassinosteroid biosynthesis gene HvDWARF5 enable balance between semi-dwarfism and maintenance of grain size in barley},

author = {Karolina Zolkiewicz and Jana Oklestkova and Brata Chmielewska and Damian Gruszka},

doi = {10.1111/ppl.70179},

issn = {0031-9317},

year  = {2025},

date = {2025-03-30},

journal = {PHYSIOLOGIA PLANTARUM},

volume = {177},

issue = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {A suitable strategy to find IAA metabolism mutants},

author = {Ruben Casanova-Sáez and Ales Pencík and Rafael Muñoz-Viana and Federica Brunoni and Rui Pinto and Ondrej Novák and Karin Ljung and Eduardo Mateo-Bonmatí},

doi = {10.1111/ppl.70166},

issn = {0031-9317},

year  = {2025},

date = {2025-03-27},

journal = { PHYSIOLOGIA PLANTARUM},

volume = {177},

issue = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {The development of a canine single-chain phage antibody library to isolate recombinant antibodies for use in translational cancer research},

author = {Malgorzata Lisowska and Erin G. Worrall and Filip Zavadil-Kokas and Keith Charlton and Euan Murray and M. Aiman Mohtar and Radovan Krejcir and Vaclav Hrabal and Jack Brydon and Ainhoa Gonzalez Urionabarrenetxea and David G. Saliba and Mariana Grima and Umesh Kalathiya and Petr Muller and Adam Krejci and Borivoj Vojtesek and Kathryn L. Ball and Robin Fahraeus and David J. Argyle and Maciej Parys and Ted R. Hupp},

doi = {10.1016/j.crmeth.2025.101008},

issn = {2667-2375},

year  = {2025},

date = {2025-03-24},

journal = {CELL REPORTS METHODS},

volume = {5},

issue = {3},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Induction of microspore embryogenesis in bread wheat by mannitol pre-treatment is associated with the disruption of endogenous hormone balance and substantial accumulation of auxins},

author = {Agnieszka Springer and Monika Krzewska and Ewa Dubas and Przemyslaw Kopec and Lenka Plakova and Karel Dolezal and Dorota Weigt and Iwona Zur},

doi = {10.1186/s12870-025-06389-x},

issn = {1471-2229},

year  = {2025},

date = {2025-03-22},

journal = { BMC PLANT BIOLOGY},

volume = {25},

issue = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Strigolactone insensitivity affects the hormonal homeostasis in barley},

author = {Magdalena Korek and Devang Mehta and Glen R. Uhrig and Agata Daszkowska-Golec and Ondrej Novak and Weronika Buchcik and Marek Marzec},

doi = {10.1038/s41598-025-94430-2},

issn = {2045-2322},

year  = {2025},

date = {2025-03-18},

journal = { SCIENTIFIC REPORTS},

volume = {15},

issue = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {THE ROLE OF PHYTOHORMONES IN PHYTOREMEDIATION USING REED},

author = {Jana Kocirova and Jana Novakova and Lenka Plackova and Iva Melcakova and Petr Hekera and Barbara Stalmachova and Peter András and Karel Dolezal},

doi = {10.26471/cjees/2025/020/324},

issn = {1842-4090},

year  = {2025},

date = {2025-03-14},

journal = { CARPATHIAN JOURNAL OF EARTH AND ENVIRONMENTAL SCIENCES},

volume = {20},

issue = {1},

pages = {183-196},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Comparative pericarp biomechanics and germination physiology of Raphanus raphanistrum and Raphanus pugioniformis indehiscent fruits},

author = {Tina Steinbrecher and Samik Bhattacharya and Jonathan Binder and Katharina Kleemeier and Felix Przesdzink and Franzicka Groene and Kyra Jacoblinnert and Klaus Mummenhoff and Gerhard Leubner-Metzger},

doi = {10.1093/aob/mcaf015},

issn = {0305-7364},

year  = {2025},

date = {2025-03-09},

journal = { ANNALS OF BOTANY},

volume = {135},

issue = {5},

pages = {977-990},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Blue or far-red light supplementation induced pre-hardening in the leaves of the Rht12 wheat dwarfing line: hormonal changes and freezing tolerance},

author = {Zsolt Gulyás and Mohamed Ahres and Tamás Pálmai and Kitti Kulman and Zahra Tahmasebi and Kalpita Singh and Kristóf Jobbágy and Danuše Tarkowská and Petre Dobrev and Radomíra Vanková and Péter Borbély and Andreas Börner and Gábor Galiba},

doi = {10.1111/ppl.70112},

year  = {2025},

date = {2025-03-03},

urldate = {2025-03-03},

journal = {PHYSIOLOGIA PLANTARUM},

volume = {177},

issue = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Plant biotechnology in the new era: From conventional methods to cutting-edge techniques},

author = {Vijay Kumar and Karel Dolezal},

doi = {10.1016/j.cpb.2025.100439},

issn = {2214-6628},

year  = {2025},

date = {2025-03-01},

journal = {CURRENT PLANT BIOLOGY},

volume = {41},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Glycosylation pathways in auxin homeostasis},

author = {Daniela Skyvarova and Federica Brunoni and Asta Zukauskaite and Ales Pencik},

doi = {10.1111/ppl.70170},

issn = {0031-9317},

year  = {2025},

date = {2025-03-01},

urldate = {2025-03-01},

journal = { PHYSIOLOGIA PLANTARUM},

volume = {177},

issue = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Gold(I) N-heterocyclic carbene complexes show strong proapoptotic , antioxidant and anti-inflammatory effects in A2780 and endothelial cells},

author = {Zdenek Trávnícek and Jan Vanco and Michal Cajan and Tomas Malina and Zdenek Dvorák and Rene Lenobel and Barbora Beláková and Johannes A. Schmid},

doi = {10.1016/j.cbi.2025.111381},

issn = {0009-2797},

year  = {2025},

date = {2025-02-25},

journal = {CHEMICO-BIOLOGICAL INTERACTIONS},

volume = {408},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Towards sustainable food packaging using natural compounds: A review of current research update},

author = {Lutfun Nahar and Emran Habibi and Georgiana-Luminita Gavril and Gamal Moustafa Mahmoud Abdelfattah and Magdalena Wrona and Cristina Nerin and Mingquan Guo and Satyajit D. Sarker},

doi = {10.1016/j.fbp.2025.01.015},

issn = {0960-3085},

year  = {2025},

date = {2025-02-21},

journal = {FOOD AND BIOPRODUCTS PROCESSING},

volume = {150},

pages = {260-274},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Correction of aberrant splicing of ELP1 pre-mRNA by kinetin derivatives - A structure activity relationship study},

author = {Barbara Makova and Vaclav Mik and Barbora Liskova and Lenka Drasarova and Martina Medvedikova and Alena Horinkova and Petr Vojta and Marek Zatloukal and Lucie Plihalova and Martin Hoenig and Karel Dolezal and Kristyna Forejt and Tomas Ozdian and Marian Hajduch and Miroslav Strnad and Jiri Voller},

doi = {10.1016/j.ejmech.2024.117176},

issn = {0223-5234},

year  = {2025},

date = {2025-02-15},

journal = {EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY},

volume = {284},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Pregnane derivatives in wheat (Triticum aestivum) and their potential role in generative development},

author = {Anna Janeczko and Jana Oklestkova and Barbara Jurczyk and Barbara Drygas},

doi = {10.1007/s10265-024-01614-4},

issn = {0918-9440},

year  = {2025},

date = {2025-02-09},

journal = { JOURNAL OF PLANT RESEARCH},

volume = {138},

issue = {2},

pages = {377-388},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Induction of α-amylase and endosperm-imposed seed dormancy: two pioneering papers in gibberellin research},

author = {Peter Hedden},

doi = {10.1007/s00425-025-04699-w},

issn = {0032-0935},

year  = {2025},

date = {2025-02-05},

journal = {PLANTA},

volume = {261},

issue = {6},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Antimicrobial potential of the leaves of Citrus grandis (L.) Osbeck collected from Iraq: Bioassay-guided isolation of sinensetin as the anti-MRSA compound},

author = {Shaymaa Al-Majmaie and Lutfun Nahar and Mukhlesur M. Rahman and George P. Sharples and Satyajit Sarker},

doi = {10.1016/j.fitote.2025.106393},

issn = {0367-326X},

year  = {2025},

date = {2025-01-30},

journal = { FITOTERAPIA},

volume = {181},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {A DOF transcriptional repressor-gibberellin feedback loop plays a crucial role in modulating light-independent seed germination},

author = {Andrea Lepri and Hira Kazmi and Gaia Bertolotti and Chiara Longo and Sara Occhigrossi and Luca Quattrocchi and Mirko De Vivo and Daria Scintu and Noemi Svolacchia and Danuse Tarkowska and Veronika Tureckova and Miroslav Strnad and Marta Del Bianco and Riccardo Di Mambro and Paolo Costantino and Sabrina Sabatini and Raffaele Dello Ioio and Paola Vittorioso },

doi = {10.1016/j.xplc.2025.101262},

year  = {2025},

date = {2025-01-27},

urldate = {2025-01-27},

journal = {Plant Communications },

volume = {6},

number = {101262},

pages = {1-17},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Effect of selected Indonesian plants on Giardia intestinalis in an experimental in vitro model},

author = {Radka Peckova and Karel Dolezal and Bohumil Sak and Dana Kvetonova and Martin Kvac and Ivan Petrik and Wisnu Nurcahyo and Ivona Foitova},

doi = {10.1186/s12906-025-04755-8},

issn = {2662-7671},

year  = {2025},

date = {2025-01-23},

journal = {BMC COMPLEMENTARY MEDICINE AND THERAPIES},

volume = {25},

issue = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Isolation, cytotoxicity evaluation, and molecular docking of 3,4,3'-tri-O-methylflavellagic acid from Anogeissus leiocarpus (DC.) Guill. & Perr. root},

author = {Yemi Adekunle, and Babutunde B. Samuel; hinemenma M. Ezeude and Lutfun Nahar and Amos A. Fatokun and , Satyajit Sarker},

doi = {10.1080/14786419.2025.2451218},

isbn = {1478-6419},

year  = {2025},

date = {2025-01-19},

journal = {NATURAL PRODUCT RESEARCH},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {3D-QSAR Design of New Bcr-Abl Inhibitors Based on Purine Scaffold and Cytotoxicity Studies on CML Cell Lines Sensitive and Resistant to Imatinib},

author = {David Cabezas and Thalia Delgado and Guisselle Sepúlveda and Petra Krnávková and Veronika Vojacková and Vladimir Krystof and Miroslav Strnad and Nicolas Ignacio Silva and Javier Echeverría and Christian Espinosa-Bustos and Guido Mellado and Jiao Luo and Jaime Mella and Cristian O. Salas},

doi = {10.3390/ph18060925},

issn = {1424-8247},

year  = {2025},

date = {2025-01-19},

urldate = {2025-01-19},

journal = {PHARMACEUTICALS},

volume = {18},

issue = {6},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Structural insights into brassinosteroid export mediated by the Arabidopsis ABC transporter ABCB1},

author = {Hong Wei and Heyuan Zhu and Wei Ying and Hilde Janssens and Miroslav Kvasnica and Johan M. Winne and Yongxiang Gao and Jiří Friml and Qian Ma and Shutang Tan and Xin Liu and Eugenia Russinova and Linfeng Sun},

doi = {10.1016/j.xplc.2024.101181},

issn = {2590-3462},

year  = {2025},

date = {2025-01-13},

journal = {Plant Communications },

volume = {6},

issue = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Sulfonation of IAA in Urtica eliminates its DR5 auxin activity},

author = {Klara Supikova and Asta Žukauskaitė and Andrea Kosinova and Aleš Pěnčík and Nuria De Diego and Lukáš Spíchal and Martin Fellner and Katerina Skorepova and Jiri Gruz},

url = {10.1007/s00299-024-03399-1},

doi = {10.1007/s00299-024-03399-1},

year  = {2025},

date = {2025-01-01},

journal = {Plant Cell Reports},

volume = {44},

number = {8},

abstract = {A novel auxin derivative, N-sulfoindole-3-acetic acid (IAA-N-SO3H, SIAA), was discovered in stinging nettle (Urtica dioica) among 116 sulfonated metabolites putatively identified by a semi-targeted UHPLC–QqTOF-MS analysis of 23 plant/algae/fungi species. These sulfometabolites were detected based on the presence of a neutral loss of sulfur trioxide, as indicated by the m/z difference of 79.9568 Da in the MS2 spectra. The structure of newly discovered SIAA was confirmed by synthesizing its standard and comparing retention time, m/z and MS2 spectrum with those of SIAA found in Urtica. To study its natural occurrence, 73 species in total were further analyzed by UHPLC–QqTOF-MS or targeted UHPLC–MS/MS method with a limit of detection of 244 fmol/g dry weight. However, SIAA was only detected in Urtica at a concentration of 13.906 ± 9.603 nmol/g dry weight. Its concentration was > 30 times higher than that of indole-3-acetic acid (IAA), and the SIAA/IAA ratio was further increased under different light conditions, especially in continuous blue light. In addition to SIAA, structurally similar metabolites, N-sulfoindole-3-lactic acid, 4-(sulfooxy)phenyllactic acid and 4-(sulfooxy)phenylacetic acid, were detected in Urtica for the first time. SIAA was biosynthesized from inorganic sulfate in seedlings, as confirmed by the incorporation of exogenous 34S-ammonium sulfate (1 mM and 10 mM). SIAA exhibited no auxin activity, as demonstrated by both the Arabidopsis DR5::GUS assay and the Arabidopsis phenotype analysis. Sulfonation of IAA may therefore be a mechanism for IAA deactivation and/or storage in Urtica, similar to sulfonation of the jasmonates in Arabidopsis.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Amide conjugates of the jasmonate precursor cis-(+)-12-oxo-phytodienoic acid regulate its homeostasis during plant stress responses},

author = {Jitka Siroka and Anita Ament and Vaclav Mik and Tomas Pospisil and Michaela Kralova and Chao Zhang and Marketa Pernisova and Michal Karady and Vladimira Nozkova and Yuho Nishizato and Takuya Kaji and Rina Saito and Mohamed Htitich and Kristyna Flokova and Claus Wasternack and Miroslav Strnad and Minoru Ueda and Ondrej Novak and Federica Brunoni},

url = {10.1093/plphys/kiae636

},

doi = {10.1093/plphys/kiae636},

issn = {0032-0889},

year  = {2024},

date = {2024-12-16},

journal = {PLANT PHYSIOLOGY},

volume = {197},

issue = {1},

abstract = {Jasmonates are a family of oxylipin phytohormones regulating plant development and growth and mediating defense versus growth responses. The upstream JA biosynthetic precursor cis-(+)-12-oxo-phytodienoic acid (cis-OPDA) acts independently of CORONATIVE INSENSITIVE 1-mediated JA signaling in several stress-induced and developmental processes. However, its perception and metabolism are only partially understood. An isoleucine analog of the biologically active JA-Ile, OPDA-Ile, was detected years ago in wounded leaves of flowering plants, opening up the possibility that conjugation of cis-OPDA to amino acids might be a relevant mechanism for cis-OPDA regulation. Here, we extended the analysis of amino acid conjugates of cis-OPDA and identified naturally occurring OPDA-Val, OPDA-Phe, OPDA-Ala, OPDA-Glu, and OPDA-Asp accumulating in response to biotic and abiotic stress in Arabidopsis (Arabidopsis thaliana). The OPDA amino acid conjugates displayed cis-OPDA-related plant responses in a JA-Ile-dependent manner. We also showed that the synthesis and hydrolysis of cis-OPDA amino acid conjugates are mediated by members of the amidosynthetase GRETCHEN HAGEN 3 and the amidohydrolase INDOLE-3-ACETYL-LEUCINE RESISTANT 1/ILR1-like families. Thus, OPDA amino acid conjugates function in the catabolism or temporary storage of cis-OPDA in stress responses instead of acting as chemical signals per se. Amide conjugate synthesis and hydrolysis control the homeostasis of a jasmonate precursor during stress responses in Arabidopsis.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Phenolic Profile of Seedless Ziziphus mauritiana Fruits and Leaves Extracts with In Vivo Antioxidant and Anti-Inflammatory Activities: Influence on Pro-Inflammatory Mediators},

author = {Arifa Khanam and Abdullah Ijaz Hussain and Esraa Haji Mohammed and Lutfun Nahar and Hassaan A. Rathore},

url = {10.1002/cbdv.202401728

},

doi = {10.1002/cbdv.202401728},

issn = {1612-1872},

year  = {2024},

date = {2024-12-12},

journal = {CHEMISTRY & BIODIVERSITY},

abstract = {The present study aimed to assess the antioxidant and anti-inflammatory activities of polyphenol-rich extracts of seedless variety of Ziziphus mauritiana (SZM). Reverse Phase High Performance Liquid Chromatography (RP-HPLC) analysis of SZM leaves and fruit extracts in ethanol revealed the presence of sixteen phenolics including chlorogenic acid, p-coumeric acid, gallic acid, kaempferol, rutin and quercetin. Leaf extract showed higher total phenolic and total flavonoid contents (177.6 mg/100 g and 46.2 mg/100 g) than in fruit extract (137.8 mg/100 g and 14.1 mg/100 g). The leaf extract exhibited higher DPPH radical-scavenging activity (63.5 %) than the fruit extract (58.2 %). The anti-inflammatory activity was evaluated on carrageenan-induced rat model and suppression of inflammatory biomarkers (Interleukin-6, Tumor necrosis factor-alpha and CRP) were studied. The fruit extract exhibited remarkable inhibition (98.1 %) at the dose level of 500 mg/kg body weight (BW), comparable to the standard drug indomethacin (98.4 %). Both extracts suppressed the inflammatory biomarkers and more pronounced results showed by the fruit extract including CRP, IL-6, and TNF-alpha. The leaf extract demonstrated the higher antioxidant potential as evident from the superoxide dismutase, catalase, malondialdehyde, glutathione peroxidase and glutathione levels. These findings suggest that SZM leaf and fruit extracts possess potential antioxidant and remarkable anti-inflammatory properties and can play a significant role in mitigating oxidative stress.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Raw biowaste conversion to high-value compounds for food, cosmetic and pharmaceutical industries},

author = {Veronika Krbeckova and Daniela Placha},

url = {10.1016/j.envres.2024.120134

},

doi = {10.1016/j.envres.2024.120134},

issn = {0013-9351},

year  = {2024},

date = {2024-12-05},

journal = {ENVIRONMENTAL RESEARCH},

volume = {263},

abstract = {Biowaste valorisation into high-value compounds is one of the main challenges of green chemistry, as chemicals produced from biological sources are identified as key substances in the development of a low-carbon and circular bioeconomy in connection with the transition from fossil to renewable feedstocks. The review summarizes the production of high-value products such as glucose-based chemicals, phenolic compounds and volatile-fatty acids prepared from biomass waste. Biowaste pretreatment methods such as milling, filtration and extraction followed by current non-catalytic methods such as microwave or ultrasound extraction and catalytic methods for the production value-added compounds in the presence of various catalyst types in conventional, nano or enzyme form are listed with a focus on value-added chemicals applied in the food, cosmetic and pharmaceutical industries. The economic feasibility, technical aspects and concept of the biorefinery are briefly mentioned, emphasizing the necessity of life cycle assessment for each bioproduct and technological process. Finally, it provides a future perspective and makes recommendations for potential research strategies, recognizing the importance of utilizing biomass waste for the production of useful compounds as an attractive and environmentally friendly approach whose development should be encouraged. The utilization of biowaste for high-value chemicals production shows high potential, however, there are still many challenges to be resolved throughout the entire production chain, reflecting technological, economic, ecological, sociological and long-term issues.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {A decoy receptor derived from alternative splicing fine-tunes cytokinin signaling in Arabidopsis},

author = {Michaela Kralova and Ivona Kubalova and Jakub Hajny and Karolina Kubiasova and Karolina Vagaska and Zengxiang Ge and Michelle Gallei and Hana Semeradova and Anna Kucharova and Martin Hoenig and Aline Monzer and Martin Kovacik and Jiri Friml and Ondrej Novak and Eva Benkova and Yoshihisa Ikeda and David Zalabak},

url = {10.1016/j.molp.2024.11.001

},

doi = {10.1016/j.molp.2024.11.001},

issn = {1674-2052},

year  = {2024},

date = {2024-12-02},

journal = {MOLECULAR PLANT},

volume = {17},

issue = {12},

pages = {1850-1865},

abstract = {Hormone perception and signaling pathways have a fundamental regulatory function in the physiological processes of plants. Cytokinins, a class of plant hormones, regulate cell division and meristem maintenance. The cytokinin signaling pathway is well established in the model plant Arabidopsis thaliana. Several negative feedback mechanisms, tightly controlling cytokinin signaling output, have been described previously. In this study, we identified a new feedback mechanism executed through alternative splicing of the cytokinin receptor AHK4/CRE1. A novel splicing variant named CRE1int'results from seventh intron retention, introducing a premature termination codon in the transcript. We showed that CRE1int' is translated in planta into a truncated receptor lacking the C-terminal receiver domain essential for signal transduction. CRE1int7 can bind cytokinin but cannot activate the downstream cascade. We present a novel negative feedback mechanism of the cytokinin signaling pathway, facilitated by a decoy receptor that can inactivate canonical cytokinin receptors via dimerization and compete with them for ligand binding. Ensuring proper plant growth and development requires precise control of the cytokinin signaling pathway at several levels. CRE1int7 represents a so-far unknown mechanism for fine-tuning the cytokinin signaling pathway in Arabidopsis.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {The potential applications of cytokinins and cytokinin oxidase/ dehydrogenase inhibitors for mitigating abiotic stresses in model and non-model plant species},

author = {Mxolisi P. Voko and Adeyemi O. Aremu and Nokwanda P. Makunga and Jaroslav Nisler and Karel Dolezal and Nqobile A. Masondo},

url = {10.1016/j.cpb.2024.100398

},

doi = {10.1016/j.cpb.2024.100398},

year  = {2024},

date = {2024-12-01},

journal = {CURRENT PLANT BIOLOGY},

volume = {40},

pages = {1-26},

abstract = {Cytokinins (CKs) are important phytohormones which are used by plants to optimize responses against abiotic stresses such as drought, salinity, temperature and nutrient stresses known to repress germination, and influencing general plant growth and development. Such stresses often trigger phenotypic plasticity and lead to low yields. Yet, the beneficial effect of CKs is counteracted by cytokinin oxidase/dehydrogenase (CKO/CKX, EC 1.5.99.12) enzymes and by N- and/or O-glycosylation. Additionally, research on CKs and CKX is often limited to model plants studied in isolation, and sparsely covers non-model plants exposed to abiotic stresses. Thus, this review explored the role of CKs and CKX inhibitors in mitigating abiotic stresses in model and non-model plants. We also examined possible crosstalk mechanisms of CKs with auxins, polyamines, and other major phytohormones. A detailed literature search was conducted using several databases including Web of Science, Google Scholar, ScienceDirect, Scopus, and PubMed. Upon perception of environmental stimuli, CKs [e.g., N6-(Delta 2isopent-2-enyl)adenine (iP), trans-zeatin (tZ) and cis-zeatin (cZ)] induce abiotic stress tolerance in a CK - dependent manner or by forming intermolecular pathways with abscisic acid, ethylene, auxins and polyamines. Regulatory motifs of type-B ARRs code for transcriptional responses via DNA-binding. Inhibitors of CKX (e.g., 3TFM-2HE, INCYDE, F-INCYDE and anisiflupurin) act as promoters of growth and stress-tolerance through the inhibition of catabolic CKXs and regulate an increase in endogenous CKs (e.g., iP, tZ and cZ) in plants. The ability of CKX inhibitors to intercept CKX gene regulation is an indication of their potential applications in agriculture and other industries that rely on plant-based products.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Comparative evaluation of the anti-proliferative effects of alkaloid-rich extract of jujube seed and paclitaxel on MDA-MB-231 breast cancer cell line},

author = {Emran Habibi and Amin Sepehrara and Hesamoddin Arabnozari and Fariborz Sharifianjazi and Seyed Ehsan Enderami and Satyajit D. Sarker and Hadi Hassannia and Lutfun Nahar},

url = {10.1016/j.jafr.2024.101438

},

doi = {10.1016/j.jafr.2024.101438},

issn = {2666-1543},

year  = {2024},

date = {2024-12-01},

journal = {JOURNAL OF AGRICULTURE AND FOOD RESEARCH},

volume = {18},

abstract = {Introduction: Cancer is the second cause of death in the world, and among all types of cancer, triple-negative breast cancer (TNBC) has one of the worst prognoses. Repeated use of chemotherapy drugs is associated with drug resistance. This study aims to investigate the effect of jujube alkaloid-rich extract on drug sensitivity and its anti-tumor effects on paclitaxel-resistant MDA-MB-231 cells in vitro. Materials and methods: The MDA-MB-231 breast cancer cell line and its paclitaxel-resistant variant were used. The XTT method was employed to measure cell viability, while Annexin-PI assays were used to detect apoptosis. Various concentrations of the jujube extract alone and in combination with paclitaxel assessed synergistic effects in 2D and 3D cell culture models. Results: The presence of alkaloids in the extract obtained from the jujube seed was confirmed by the dragendorff test and its amount was 0.395 g in 5.0 g of jujube seed powder. Paclitaxel and jujube extract exhibited dosedependent cytotoxic effects on paclitaxel-resistant MDA-MB-231 cells. The combination of jujube extract and paclitaxel significantly decreased IC50 from 541.3 mu g/mL to 124.3 mu g/mL, and the IC20 decreased from 92.1 mu g/ mL to 23.9 mu g/mL when combined with paclitaxel in 2D culture model. The combination's toxicity was reduced in the 3D culture model. Apoptosis rates were 0.75 % in the control group, 13.89 % in the alkaloid-rich extract group, 2.59 % in the paclitaxel group, and 42.90 % in the combination group. Conclusion: Combining paclitaxel with alkaloid-rich jujube seed extract enhances cytotoxicity and apoptosis in paclitaxel-resistant MDA-MB-231 breast cancer cells in both 2D and 3D culture models. This suggests that such combinations might be a viable strategy to overcome drug resistance in TNBC treatments.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Misincorporations of amino acids in p53 in human cells at artificially constructed termination codons in the presence of the aminoglycoside Gentamicin},

author = {Kamila Pawlicka and Tomas Henek and Lukas Uhrik and Lenka Hernychova and Monikaben Padariya and Jakub Faktor and Slawomir Makowiec and Borivoj Vojtesek and David Goodlett and Ted Hupp and Umesh Kalathiya},

url = {10.3389/fgene.2024.1407375

},

doi = {10.3389/fgene.2024.1407375},

year  = {2024},

date = {2024-11-05},

journal = {FRONTIERS IN GENETICS},

volume = {15},

abstract = {Readthrough of a translation termination codon is regulated by ribosomal A site recognition and insertion of near-cognate tRNAs. Small molecules exist that mediate incorporation of amino acids at the stop codon and production of full-length, often functional protein but defining the actual amino acid that is incorporated remains a challenging area. Herein, we report on the development a human cell model that can be used to determine whether rules can be developed using mass spectrometry that define the type of amino acid that is placed at a premature termination codon (PTC) during readthrough mediated by an aminoglycoside. The first PTC we analyzed contained the relatively common cancer-associated termination signal at codon 213 in the p53 gene. Despite of identifying a tryptic peptide with the incorporation of an R at codon 213 in the presence of the aminoglycoside, there were no other tryptic peptides detected across codon 213 that could be recovered; hence we constructed a more robust artificial PTC model. P53 expression plasmids were developed that incorporate a string of single synthetic TGA (opal) stop codons at S127P128A129 within the relatively abundant tryptic p53 peptide 121-SVTCTYSPALNK-132. The treatment of cells stably expressing the p53-TGA129 mutation, treated with Gentamicin, followed by immunoprecipitation and trypsinization of p53, resulted in the identification R, W, or C within the tryptic peptide at codon-TGA129; as expected based on the two-base pairing of the respective anticodons in the tRNA to UGA, with R being the most abundant. By contrast, incorporating the amber or ochre premature stop codons, TAA129 or TAG129 resulted in the incorporation of a Y or Q amino acid, again as expected based on the two base pairings to the anticodons, with Q being the most abundant. A reproducible non-canonical readthrough termination codon-skip event at the extreme C-terminus at codon 436 in the SBP-p53 fusion protein was detected which provided a novel assay for non-canonical readthrough at an extreme C-terminal PTC. The incorporation of amino acids at codons 127, 128, or 129 generally result in a p53 protein that is predicted to be 'unfolded' or inactive as defined by molecular dynamic simulations presumably because the production of mixed wild-type p53 and mutant oligomers are known to be inactive through dominant negative effects of the mutation. The data highlight the need to not only produce novel small molecules that can readthrough PTCs or C-terminal termination codons, but also the need to design methods to insert the required amino acid at the position that could result in a 'wild-type' functional protein.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Sucrose-responsive osmoregulation of plant cell size by a long non-coding RNA},

author = {Jakub Hajny and Tereza Travnickova and Martina Spundova and Michelle Roenspies and R. M. Imtiaz Karim Rony and Sebastian Sacharowski and Michal Krzyszton and David Zalabak and Christian S. Hardtke and Ales Pecinka and Holger Puchta and Szymon Swiezewski and Jaimie M. van Norman and Ondrej Novak},

url = {10.1016/j.molp.2024.09.011

},

doi = {10.1016/j.molp.2024.09.011},

issn = {1674-2052},

year  = {2024},

date = {2024-11-04},

journal = {MOLECULAR PLANT},

volume = {17},

issue = {11},

pages = {1719-1732},

abstract = {In plants, sugars are the key source of energy and metabolic building blocks. The systemic transport of sugars is essential for plant growth and morphogenesis. Plants evolved intricate molecular networks to effectively distribute sugars. The dynamic distribution of these osmotically active compounds is a handy tool for regulating cell turgor pressure, an instructive force in developmental biology. In this study, we have investigated the molecular mechanism behind the dual role of the receptor-like kinase CANAR. We functionally characterized a long non-coding RNA, CARMA, as a negative regulator of CANAR. Sugar- responsive CARMA specifically fine-tunes CANAR expression in the phloem, the route of sugar transport. Our genetic, molecular, microscopy, and biophysical data suggest that the CARMA-CANAR module controls the shoot-to-root phloem transport of sugars, allows cells to flexibly adapt to the external osmolality by appropriate water uptake, and thus adjust the size of vascular cell types during organ growth and development. Our study identifies a nexus of plant vascular tissue formation with cell internal pressure monitoring, revealing a novel functional aspect of long non-coding RNAs in developmental biology.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {BBX21 Integrates Brassinosteroid Biosynthesis and Signaling in the Inhibition of Hypocotyl Growth under Shade},

author = {Gabriel Gomez-Ocampo and Carlos D. Crocco and Jimena Cascales and Jana Oklestkova and Danuse Tarkowska and Miroslav Strnad and Santiago Mora-Garcia and Jose L. Pruneda-Paz and Miguel A. Blazquez and Javier F. Botto},

url = {10.1093/pcp/pcad126

},

doi = {10.1093/pcp/pcad126},

issn = {0032-0781},

year  = {2024},

date = {2024-11-01},

journal = {PLANT AND CELL PHYSIOLOGY},

volume = {65},

issue = {10},

pages = {1627-1639},

abstract = {B-Box-containing zinc finger transcription factors (BBX) are involved in light-mediated growth, affecting processes such as hypocotyl elongation in Arabidopsis thaliana. However, the molecular and hormonal framework that regulates plant growth through BBX proteins is incomplete. Here, we demonstrate that BBX21 inhibits the hypocotyl elongation through the brassinosteroid (BR) pathway. BBX21 reduces the sensitivity to 24-epiBL, a synthetic active BR, principally at very low concentrations in simulated shade. The biosynthesis profile of BRs showed that two active BR-brassinolide and 28-homobrassinolide-and 8 of 11 intermediates can be repressed by BBX21 under white light (WL) or simulated shade. Furthermore, BBX21 represses the expression of CYTOCHROME P450 90B1 (DWF4/CYP90B1), BRASSINOSTEROID-6-OXIDASE 1 (BR6OX1, CYP85A1) and BR6OX2 (CYP85A2) genes involved in the BR biosynthesis in WL while specifically promoting DWF4 and PHYB ACTIVATION TAGGED SUPPRESSOR 1 (CYP2B1/BAS1) expression in WL supplemented with far-red (WL + FR), a treatment that simulates shade. In addition, BBX21 represses BR signaling genes, such as PACLOBUTRAZOL RESISTANCE1 (PRE1), PRE3 and ARABIDOPSIS MYB-LIKE 2 (MYBL2), and auxin-related and expansin genes, such as INDOLE-3-ACETIC ACID INDUCIBLE 1 (IAA1), IAA4 and EXPANSIN 11 in short-term shade. By a genetic approach, we found that BBX21 acts genetically upstream of BRASSINAZOLE-RESISTANT 1 (BZR1) for the promotion of DWF4 and BAS1 gene expression in shade. We propose that BBX21 integrates the BR homeostasis and shade-light signaling, allowing the fine-tuning of hypocotyl elongation in Arabidopsis.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Phytochemical analysis and immune-modulatory potential of Trichaptum biforme polysaccharides: Implications for cancer},

author = {Emran Habibi and Parsa Hemmati and Hesamoddin Arabnozari and Hasti Asadi Khalili and Fariborz Sharifianjazi and Seyed Ehsan Enderami and Satyajit D. Sarker and Hadi Hassannia and Lutfun Nahar},

url = {10.1016/j.ijbiomac.2024.135691

},

doi = {10.1016/j.ijbiomac.2024.135691},

issn = {0141-8130},

year  = {2024},

date = {2024-11-01},

journal = {INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES},

volume = {280},

abstract = {Cancer remains a major global health concern, often challenging traditional treatments. Natural compounds like fungal polysaccharides have gained attention for their immune-modulatory properties. This study evaluates the phytochemical properties of the n-hexane fraction of Trichaptum biforme and explores its immune-enhancing effects. The study involved isolating three sterol derivatives using column chromatography and purifying polysaccharides from T. biforme (TBP) through hot aqueous extraction. TBP content was quantified via the phenol-sulfuric acid method, and antioxidant activity was assessed using DPPH and FRAP assays. Cytotoxicity of TBP on THP-1 cells and the impact on IL-1(3 and TNF-alpha secretion were evaluated through the XTT assay. Flow cytometry and ELISA assessed cytotoxic activity and IFN-gamma secretion in NK cells. The compound 9, 11-Dehydroergosterol peroxide was identified for the first time in T. biforme. . The total polysaccharide content was 78.18 +/- 0.81 %. The TBP significantly increased IL-1(3 and TNF-alpha secretion from THP-1 cells at concentrations of 10 and 320 mu g/mL (p p < 0.01). Treatment of NK cells with the extract (320 g/mL) and IL-2 (100 units/mL) significantly enhanced cytotoxic activity and IFN-gamma secretion compared to the control group (p < 0.01). These findings suggest that TBP holds promise as a candidate for bolstering anticancer immune responses.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Amides of moronic acid and morolic acid with the tripeptides MAG and GAM targeting antimicrobial, antiviral and cytotoxic effects},

author = {Uladzimir Bildziukevich and Lucie Černá and Jana Trylčová and Marie Kvasnicová and Lucie Rárová and David Šaman and Petra Lovecká and Jan Weber and Zdeněk Wimmer},

doi = {10.1039/d4md00742e},

year  = {2024},

date = {2024-10-29},

journal = {RSC Medicinal Chemistry},

volume = {16},

issue = {2},

pages = {801-811},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Oleanolic acid purified from the stem bark of Olax subscorpioidea Oliv. inhibits the function and catalysis of human 17β-hydroxysteroid dehydrogenase 1},

author = {Yemi A. Adekunle and Babatunde B. Samuel and Wande M. Oluyemi and Adeniyi T. Adewumi and Salerwe Mosebi and Lutfun Nahar and Amos A. Fatokun and Satyajit D. Sarker},

url = {10.1080/07391102.2024.2423173},

doi = {10.1080/07391102.2024.2423173},

isbn = {0739-1102},

year  = {2024},

date = {2024-10-28},

journal = {JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS},

abstract = {Cancer is a leading cause of global death. Medicinal plants have gained increasing attention in cancer drug discovery. In this study, the stem bark extract of Olax subscorpioidea, which is used in ethnomedicine to treat cancer, was subjected to phytochemical investigation leading to the isolation of oleanolic acid (OA). The structure was elucidated by 1-dimensional and 2-dimensional nuclear magnetic resonance spectroscopic (NMR) data, and by comparing its data with previously reported data. Molecular docking was used to investigate the interactions of OA with nine selected cancer-related protein targets. OA docked well with human 17 beta-hydroxysteroid dehydrogenase type-1 (17 beta HSD1), caspase-3, and epidermal growth factor receptor tyrosine kinase (binding affinities: -9.8, -9.3, and -9.1 kcal/mol, respectively). OA is a triterpenoid compound with structural similarity to steroids. This similarity with the substrates of 17 beta HSD1 gives the inhibitor candidate an excellent opportunity to bind to 17 beta HSD1. The structural and functional dynamics of OA-17 beta HSD1 were investigated by molecular dynamics simulations at 240 ns. Molecular mechanics/Poisson-Boltzmann surface area (MMPBSA) studies showed that OA had a binding free energy that is comparable with that of vincristine (-52.76, and -63.56 kcal/mol, respectively). The average C-alpha root mean square of deviation (RMSD) value of OA (1.69 & Aring;) compared with the unbound protein (2.01 & Aring;) indicated its high stability at the protein's active site. The binding energy and stability at the active site of 17 beta HSD1 recorded in this study indicate that OA exhibited profound inhibitory potential. OA could be a good scaffold for developing new anti-breast cancer drugs.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {In Vitro Cytotoxicity and Antimicrobial Activity against Acne-Causing Bacteria and Phytochemical Analysis of Galangal (Alpinia galanga) and Bitter Ginger (Zingiber zerumbet) Extracts},

author = {Nongkhai, Tanat Na; Maddocks, Sarah E.; Phosri, Santi; Sangthong, Sarita; Pintathong, Punyawatt; Chaiwut, Phanuphong; Chandarajoti, Kasemsiri; Nahar, Lutfun; Sarker, Satyajit D.; Theansungnoen, Tinnakorn },

url = {10.3390/ijms252010869

},

doi = {10.3390/ijms252010869},

issn = {1661-6596},

year  = {2024},

date = {2024-10-10},

journal = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES},

volume = {25},

issue = {20},

abstract = {Galangal (Alpinia galanga (L.) Willd) and bitter ginger (Zingiber zerumbet (L.) Roscoe) are aromatic rhizomatous plants that are typically used for culinary purposes. These rhizomatous plants have many biological properties and the potential to be beneficial for pharmaceutics. In this study, we evaluated the antioxidant and antimicrobial activities, with a specific focus on acne-causing bacteria, as well as the phytochemical constituents, of different parts of galangal and bitter ginger. The rhizomes, stems, and leaves of galangal and bitter ginger were separately dried for absolute ethanol and methanol extractions. The extracts were used to evaluate the antioxidant activity using a DPPH radical scavenging assay (0.005-5000 mu g/mL), antimicrobial activity against acne-causing bacteria (0.50-31.68 mg/mL), and in vitro cytotoxicity toward human keratinocytes and fibroblasts (62.5-1000 mu g/mL), as well as analyses of bioactive phytochemicals via GC-MS and LC-MS/MS (500 ppm). The ethanol and methanol extracts of bitter ginger and galangal's rhizomes (BRhE, BRhM, GRhE, and GRhM), stems (BStE, BStM, GRhE, and GRhM), and leaves (BLeE, BLeM, GLeE, and GLeM), respectively, showed antioxidant and antimicrobial activities. The extracts of all parts of bitter ginger and galangal were greatly antioxidative with 0.06-1.42 mg/mL for the IC50 values, while most of the extracts were strongly antimicrobial against C. acnes DMST 14916, particularly BRhM, BRhE, GRhM, and GRhE (MICs: 3.96-7.92 mg/mL). These rhizome extracts had also antimicrobial activities against S. aureus TISTR 746 (MICs: 7.92-31.68 mg/mL) and S. epidermidis TISTR 518 (MICs: 7.92-15.84 mg/mL). The extracts of bitter ginger and galangal rhizomes were not toxic to HaCaT and MRC-5 even at the highest concentrations. Through GC-MS and LC-MS/MS analysis, phytochemicals in bitter ginger rhizome extracts, including zerumbone, tectorigenin, piperic acid, demethoxycurcumin, and cirsimaritin, and galangal rhizome extracts, including sweroside and neobavaisoflavone, were expected to provide the antioxidant and anti-microbial activities. Therefore, the results suggest that the bitter ginger and galangal extracts could be natural anti-acne compounds with potential for pharmaceutic, cosmetic, and aesthetic applications.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Emerging pharmacological approaches for Huntington's disease},

author = {Kuldeep Singh and Divya Jain and Pranshul Sethi and Jeetendra Kumar Gupta and Arpan Kumar Tripathi and Shivendra Kumar and Satyajit D. Sarker and Lutfun Nahar and Ajay Guru},

url = {10.1016/j.ejphar.2024.176873

},

doi = {10.1016/j.ejphar.2024.176873},

issn = {0014-2999},

year  = {2024},

date = {2024-10-05},

journal = {EUROPEAN JOURNAL OF PHARMACOLOGY},

volume = {980},

abstract = {Huntington's disease (HD) is a progressive neurodegenerative disorder characterized by cognitive, motor, and psychiatric symptoms. Despite significant advances in understanding the underlying molecular mechanisms of HD, there is currently no cure or disease-modifying treatment available. Emerging pharmacological approaches offer promising strategies to alleviate symptoms and slow down disease progression. This comprehensive review aims to provide a critical appraisal of the latest developments in pharmacological interventions for HD. The review begins by discussing the pathogenesis of HD, focusing on the role of mutant huntingtin protein, mitochondrial dysfunction, excitotoxicity, and neuro-inflammation. It then explores emerging therapeutic targets, including the modulation of protein homeostasis, mitochondrial function, neuro-inflammation, and neurotransmitter systems. Pharmacological agents targeting these pathways are discussed, including small molecules, gene-based therapies, and neuroprotective agents. In recent years, several clinical trials have been conducted to evaluate the safety and efficiency of novel compounds for HD. This review presents an update on the outcomes of these trials, highlighting promising results and challenges encountered. Additionally, it discusses the potential of repurposing existing drugs approved for other indications as a cost-effective approach for HD treatment. The review concludes by summarizing the current state of pharmacological approaches for HD and outlining future directions in drug development. The integration of multiple therapeutic strategies, personalized medicine approaches, and combination therapies are highlighted as potential avenues to maximize treatment effectiveness.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Novel neuroprotective 5,6-dihydropyrido[2′,1':2,3]imidazo[4,5-c] quinoline derivatives acting through cholinesterase inhibition and CB2 signaling modulation},

author = {Sushovan Jena and Gabriel Gonzalez and Dominik Vitek and Marie Kvasnicova and Sarka Stepankova and Miroslav Strnad and Jiri Voller and Kaushik Chanda},

url = {10.1016/j.ejmech.2024.116592

},

doi = {10.1016/j.ejmech.2024.116592},

issn = {0223-5234},

year  = {2024},

date = {2024-10-05},

journal = {EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY},

volume = {276},

abstract = {A novel group of 5,6-dihydropyrido [2 ',1':2,3]imidazo [4,5-c]quinolines was prepared via a microwave assisted one-pot telescopic approach. The synthetic sequence involves the formation of an amine precursor of imidazo [1,2-a]pyridine via condensation and reduction under microwave irradiation. Subsequently, the Pictet-Spengler cyclisation reaction occurs with ketones (cyclic or acyclic) to obtain substituted 5,6-dihydropyrido [2 ',1':2,3] imidazo [4,5-c]quinolines in excellent yields. The compounds were tested as neuroprotective agents. Observed protection of neuron-like cells, SH-SY5Y differentiated with ATRA, in Parkinson's and Huntington's disease models inspired further mechanistic studies of protective activity against damage induced by 1-methyl-4-phenylpyridinium (MPP+), a compound causing Parkinson's disease. The novel compounds exhibit similar or higher potency than ebselen, an established drug with antioxidant activity, in the cells against MPP + -induced total cellular superoxide production and cell death. However, they exhibit a significantly higher capacity to reduce mitochondrial superoxide and preserve mitochondrial membrane potential. We also observed marked differences between a selected derivative and ebselen in terms of normalizing MPP + -induced phosphorylation of Akt and ERK1/2. The cytoprotective activity was abrogated when signaling through cannabinoid receptor CB2 was blocked. The compounds also inhibit both acetylcholine and butyrylcholine esterases. Overall the data show that novel 5,6-dihydropyrido [2 ',1':2,3]imidazo [4,5-c]quinoline have a broad cytoprotective activity which is mediated by several mechanisms including mitoprotection.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {The Bioprotective Effects of Marigold Tea Polyphenols on Obesity and Oxidative Stress Biomarkers in High-Fat-Sugar Diet-Fed Rats},

author = {Bader Alsuwayt and Neelam Iftikhar and Abdullah Ijaz Hussain and Ashfaq Ahmad and Irsa Zafar and Arifa Khanam and Wen-Nee Tan and Lutfun Nahar and Afaf F. Almuqati and Esraa Mohammad Haji and Ali F. Almutairy and Satyajit D. Sarker},

url = {10.1155/2024/3833521

},

doi = {10.1155/2024/3833521},

issn = {1755-5914},

year  = {2024},

date = {2024-10-04},

journal = {CARDIOVASCULAR THERAPEUTICS},

abstract = {Background: The research is aimed at exploring the potential of marigold petal tea (MPT), rich in polyphenol contents, against oxidative stress and obesity in a rat model following a high-fat-sugar diet (HFSD).Methods: The MPT was prepared through the customary method of decoction and was subjected to analysis for its polyphenol composition using reversed-phase high-performance liquid chromatography (RP-HPLC). Two specific doses of MPT, namely, 250 and 500 mg/kg body weight (BW), were chosen for the study-referred to as MPT-250 and MPT-500, respectively.Result: The main phenolic acids and flavonoids identified in MPT, with concentrations exceeding 10 mg/100 mL of tea, included catechin, rutin, salicylic acid, gallic acid, sinapic acid, chlorogenic acid, cinnamic acid, and ellagic acid. The total phenolic (TP) and total flavonoid (TF) contents in MPT were measured to be 5.53 and 7.73 mg/g, respectively. Additionally, MPT demonstrated a 57.2% scavenging capacity with 2,2-diphenyl-1-picrylhydrazyl radical. Notably, the administration of a higher dose (MPT-500) showed a significant reduction in body mass index (BMI) and a 51.24% reduction in the rate of increase in BW compared to the HFSD group. The findings indicated that all the treatment groups, that is, orlistat treatment (OT), MPT-250, and MPT-500 groups, experienced reduced levels of serum total cholesterol (TC), triglyceride (TG), and markers of lipoproteins in contrast to the HFSD group. Moreover, MPT helped restore the levels of malondialdehyde (MDA), superoxide dismutase (SOD), and reduced glutathione (GSH), thereby demonstrating its potential in combating oxidative stress. The MPT-500 group also displayed decreased liver and kidney weights and an improved atherogenic index when compared to the HFSD group.Conclusion: The results clearly indicate that a high dosage of MPT showed antiobesity activity which was comparable to the same effects produced by the conventional drug orlistat.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Cytotoxic Flavonoids from Lannea egregia Engl. & K. Krause},

author = {Oluwatosin T. Ayodele and Olaoluwa O. Olaoluwa and Saheed O. Benson and Olapeju O. Aiyelaagbe and Lutfun Nahar and Amos A. Fatokun and Satyajit D. Sarker},

issn = {1735-403X},

year  = {2024},

date = {2024-10-01},

journal = {PHARMACEUTICAL SCIENCES},

volume = {30},

issue = {4},

pages = {496-501},

abstract = {Background: Lannea egregia Engl. & K. Krause (family: Anacardiaceae) is a well-known medicinal plant in Nigeria whose various parts have been shown to elicit several biological activities. This study specifically explored the leaf of L. egregia for potential cytotoxic compounds. Methods: n-Hexane, dichloromethane and methanolic extracts of the leaf were prepared using the Soxhlet apparatus and concentrated using the rotary evaporator. Compounds were isolated by reversed-phase preparative high-performance liquid chromatography, and the structures were determined by spectroscopic means. The methanolic extract and the isolated compounds were screened for cytotoxicity against HeLa and MCF-7 cancer cell lines, using the MTT assay. Results: Three flavonoids, myricetin (1), myricetin 3-O-alpha-L-rhamnoside (2) and quercetin 3-O-alpha-L-rhamnoside (3), were isolated from the methanolic extract of the leaf of L. egregia. The methanolic extract and compound 3 showed the most potent inhibition profiles against the cells, with IC50 values (Mean f SEM) of 45.3 f 1.5 mu g/mL and 57.5 f 0.4 mu g/mL for the methanolic extract, and 36.5 f 2.0 mu M and 57.9 f 10.1 mu M for compound 3, against HeLa and MCF-7 cells, respectively. Conclusion:

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Effects of Zinc Phthalocyanine Photodynamic Therapy on Vital Structures and Processes in Hela Cells},

author = {Jakub Hosik and Barbora Hosikova and Svatopluk Binder and Rene Lenobel and Marketa Kolarikova and Lukas Malina and Hanna Dilenko and Katerina Langova and Robert Bajgar and Hana Kolarova},

url = {10.3390/ijms251910650

},

doi = {10.3390/ijms251910650},

issn = {1661-6596},

year  = {2024},

date = {2024-10-01},

journal = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES},

volume = {25},

issue = {19},

abstract = {This work presents results on the efficiency of newly designed zinc phthalocyanine-mediated photodynamic therapy of both tumoral and nontumoral cell models using the MTT assay. Further detailed examinations of mechanistic and cell biological effects were focused on the HELA cervical cancer cell model. Here, ROS production, changes in the mitochondrial membrane potential, the determination of genotoxicity, and protein changes determined by capillary chromatography and tandem mass spectrometry with ESI were analyzed. The results showed that, in vitro, 5 Jcm-2 ZnPc PDT caused a significant increase in reactive oxygen species. Still, except for superoxide dismutase, the levels of proteins involved in cell response to oxidative stress did not increase significantly. Furthermore, this therapy damaged mitochondrial membranes, which was proven by a more than 70% voltage-dependent channel protein 1 level decrease and by a 65% mitochondrial membrane potential change 24 h post-therapy. DNA impairment was assessed by an increased level of DNA fragmentation, which might be related to the decreased level of DDB1 (decrease in levels of more than 20% 24 h post-therapy), a protein responsible for maintaining genomic integrity and triggering the DNA repair pathways. Considering these results and the low effective concentration (LC50 = 30 nM), the therapy used is a potentially very promising antitumoral treatment.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {In Vitro Interaction of Binuclear Copper Complexes with Liver Drug-Metabolizing Cytochromes P450},

author = {Alena Spicakova and Zuzana Horackova and Pavel Kopel and Pavel Anzenbacher},

url = {10.3390/ph17091194

},

doi = {10.3390/ph17091194},

issn = {1424-8247},

year  = {2024},

date = {2024-09-10},

urldate = {2024-09-10},

journal = {PHARMACEUTICALS},

volume = {17},

issue = {9},

pages = {1-11},

abstract = {Two copper(II) mixed ligand complexes with dicarboxylate bridges were prepared and studied, namely [Cu-2(mu-fu)(pmdien)(2)(H2O)(2)](ClO4)(2) (complex No. 5) and [Cu-2(mu-dtdp)(pmdien)(2)(H2O)(2)](ClO4)(2) (complex No. 6), where H(2)fu = fumaric acid, pmdien = N,N,N ',N '',N '' pentamethyldiethylenetriamine, and H(2)dtdp = 3,3 '-dithiodipropionic acid. The copper atoms are coordinated in the same mode by the tridentate pmdien ligand and oxygen of water molecules, and they only differ in the dicarboxylate bridge. This work is focused on the study of the inhibitory effect of these potential antimicrobial drugs on the activity of the most important human liver drug-metabolizing enzymes, cytochromes P450 (CYP), especially their forms CYP2C8, CYP2C19, and CYP3A4. The obtained results allow us to estimate the probability of potential drug interactions with simultaneously administrated drugs that are metabolized by these CYP enzymes. In conclusion, the presence of adverse effects due to drug-drug interactions with concomitantly used drugs cannot be excluded, and hence, topical application may be recommended as a relatively safe approach.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Phytochemistry and therapeutic potential of the genus Asphodelus L.: an update},

author = {Lutfun Nahar and Afaf Al Groshi and Lakshmi Thangavelu and Fyaz M. D. Ismail and Andrew R. Evans and Satyajit D. Sarker},

url = {10.1007/s11101-024-10004-6

},

doi = {10.1007/s11101-024-10004-6},

issn = {1568-7767},

year  = {2024},

date = {2024-09-06},

journal = {PHYTOCHEMISTRY REVIEWS},

abstract = {The genus Asphodelus L. (family: Asphodelaceae) comprises ca. 20 hardy herbaceous, perennial flowering plant species, and is native to Africa, the Indian subcontinent, the Mediterranean region, and temperate Europe, and naturalized in Australia, Mexico, New Zealand, and some parts of the USA. The Asphodelus species have long been used as traditional medicines for the treatment of acne, alopecia, burns, earache, eczema, local inflammation, psoriasis, and toothache. Since the publication of the last review in 2019, which essentially covered the published literature until the end of 2018, several articles have been published reporting the identification of various secondary metabolites including anthraquinones, flavonoids and phenolic acids and demonstrating the therapeutic potential of the genus Asphodelus. This review article critically appraises the literature on this genus published during 2019-2023 on therapeutic potential and phytochemistry. During this period, among the identified compounds from various Asphodelus species, including A. aestivus Brot., A. albus Mill., A. bento-rainhae P. Silva, A. fistulosus L., A. macrocarpus Salzm. Viv., A. ramosus L. and A. tenuifolius Cav., the phytochemicals of the classes of anthraquinones, flavonoids and phenolic acids were the most dominating ones. Numerous studies established the therapeutic potential of the Asphodelus species mainly against cancer, diabetes, microbial infections, and various ailments caused by oxidative stress. None or negligible toxicity could be observed in the toxicological studies, suggesting an acceptable level of safety of Asphodelus products for potential therapeutic applications.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Caffeine-Legal Natural Stimulant with Open Research Perspective: Spectroscopic and Theoretical Characterization},

author = {Teobald Kupka and Natalina Makieieva and Michal Jewginski and Magdalena Witek and Barbara Blicharska and Oimahmad Rahmonov and Karel Dolezal and Tomas Pospisil},

url = {10.3390/molecules29184382

},

doi = {10.3390/molecules29184382},

issn = {1420-3049},

year  = {2024},

date = {2024-09-01},

journal = {MOLECULES},

volume = {29},

issue = {18},

abstract = {Caffeine is an alkaloid with a purine structure and has been well known for centuries due to its presence in popular drinks-tea and coffee. However, the structural and spectroscopic parameters of this compound, as well as its chemical and biological activities, are still not fully known. In this study, for the first time, we report on the measured oxygen-17 NMR spectra of this stimulant. To support the assignment of our experimental NMR data, extensive quantum chemical calculations of NMR parameters, including nuclear magnetic shielding constants and indirect spin-spin coupling constants, were performed. In a theoretical study, using nine efficient density functionals (B3LYP, BLYP, BP86, CAM-B3LYP, LC-BLYP, M06, PBE0, TPSSh, wB97x), and in combination with a large and flexible correlation-consistent aug-cc-pVTZ basis set, the structure and NMR parameters were predicted for a free molecule of caffeine and in chloroform, DMSO and water. A polarized continuum model (PCM) was used to include a solvent effect. As a result, an optimal methodology was developed for predicting reliable NMR data, suitable for studies of known, as well as newly discovered, purines and similar alkaloids. The results of the current work could be used in future basic and applied studies, including NMR identification and intermolecular interactions of caffeine in various raw materials, like plants and food, as well as in the structural and spectroscopic characterization of new compounds with similar structures.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Atropisomeric 1-phenylbenzimidazoles affecting microtubule organization: influence of axial chirality},

author = {Jana Pospisilova and Tomas Heger and Ondrej Kurka and Marie Kvasnicova and Anna Chladkova and Ivan Nemec and Lucie Rarova and Petr Cankar},

url = {10.1039/d4ob00863d},

doi = {10.1039/d4ob00863d},

issn = {1477-0520},

year  = {2024},

date = {2024-08-28},

journal = {ORGANIC & BIOMOLECULAR CHEMISTRY},

volume = {22},

issue = {34},

abstract = {Benzimidazoles are frequently used in medicinal chemistry. Their anticancer effect is among the most prominent biological activities exhibited by this scaffold. Although numerous benzimidazole derivatives have been synthesized, possible atropisomerism of ortho-substituted 1-phenylbenzimidazoles has been largely overlooked. The aim of this research was to synthesize a small library of novel atropisomeric benzimidazole derivatives and explore their biological activity in various cancer and normal human cell lines. The new unique structural motif provides an interesting 3D architecture with axial chirality, which further contributes to molecular complexity and specificity. Racemates and their separated atropisomers arrested the cell cycle, caused apoptosis, and affected microtubule organization in cancer cells in vitro at different intensities. Moreover, this phenomenon was also verified by the inhibition of endothelial cell migration. These results showed that (+)-atropisomers, especially 5n, exhibit a stronger effect and show promise as agents for cancer therapy. Axially chiral benzimidazoles affects microtubule organization.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {AAO2 impairment enhances aldehyde detoxification by AAO3 in Arabidopsis leaves exposed to UV-C or Rose-Bengal},

author = {Zhadyrassyn Nurbekova and Sudhakar Srivastava and Zai Du Nja and Kusum Khatri and Jaykumar Patel and Babita Choudhary and Veronica Tureckova and Miroslav Strand and Edyta Zdunek-Zastocka and Rustem Omarov and Dominic Standing and Moshe Sagi},

url = {10.1111/tpj.16985

},

doi = {10.1111/tpj.16985},

issn = {0960-7412},

year  = {2024},

date = {2024-08-27},

journal = {PLANT JOURNAL},

volume = {120},

issue = {1},

pages = {272-288},

abstract = {Among the three active aldehyde oxidases in Arabidopsis thaliana leaves (AAO1-3), AAO3, which catalyzes the oxidation of abscisic-aldehyde to abscisic-acid, was shown recently to function as a reactive aldehyde detoxifier. Notably, aao2KO mutants exhibited less senescence symptoms and lower aldehyde accumulation, such as acrolein, benzaldehyde, and 4-hydroxyl-2-nonenal (HNE) than in wild-type leaves exposed to UV-C or Rose-Bengal. The effect of AAO2 expression absence on aldehyde detoxification by AAO3 and/or AAO1 was studied by comparing the response of wild-type plants to the response of single-functioning aao1 mutant (aao1S), aao2KO mutants, and single-functioning aao3 mutants (aao3Ss). Notably, aao3Ss exhibited similar aldehyde accumulation and chlorophyll content to aao2KO treated with UV-C or Rose-Bengal. In contrast, wild-type and aao1S exhibited higher aldehyde accumulation that resulted in lower remaining chlorophyll than in aao2KO leaves, indicating that the absence of active AAO2 enhanced AAO3 detoxification activity in aao2KO mutants. In support of this notion, employing abscisic-aldehyde as a specific substrate marker for AAO3 activity revealed enhanced AAO3 activity in aao2KO and aao3Ss leaves compared to wild-type treated with UV-C or Rose-Bengal. The similar abscisic-acid level accumulated in leaves of unstressed or stressed genotypes indicates that aldehyde detoxification by AAO3 is the cause for better stress resistance in aao2KO mutants. Employing the sulfuration process (known to activate aldehyde oxidases) in wild-type, aao2KO, and molybdenum-cofactor sulfurase (aba3-1) mutant plants revealed that the active AAO2 in WT employs sulfuration processes essential for AAO3 activity level, resulting in the lower AAO3 activity in WT than AAO3 activity in aao2KO.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {A New Abscisic Acid Conjugate, ABA-L-Glutamate, Determined in Different Plant Species by Combined Immunoaffinity Chromatography-Tandem Mass Spectrometry},

editor = {Veronika Tureckova and Jana Oklestkova and Asta Zukauskaite and Ludek Eyer and Ondrej Novak and Miroslav Strnad},

url = {10.1007/s00344-024-11436-2

},

doi = {10.1007/s00344-024-11436-2},

issn = {0721-7595},

year  = {2024},

date = {2024-08-21},

journal = {JOURNAL OF PLANT GROWTH REGULATION},

volume = {43},

pages = {4810-4825},

abstract = {Abscisic acid (ABA) is a phytohormone that occurs in plants at very low concentration (pmol/g fresh weight) and regulates multiple biological processes, including stomatal closure, seed germination, and responses to environmental stresses. In the present study, isolation of ABA, ABA glucosyl ester, and 11 ABA amino acid conjugates from minute quantities of plant tissue (less than 20 mg fresh weight) was achieved using a purification method based on the combination of an Oasis HLB column and an immunoaffinity sorbent. New monoclonal antibodies raised against (+)-cis,trans-ABA conjugated to BSA through its carboxyl group (C1) were characterised by enzyme-linked immunosorbent assay (ELISA) and used for immunoaffinity chromatography (IAC) gel preparation. The use of immunoaffinity purification significantly reduced matrix effects and increased the selectivity and sensitivity of subsequent UHPLC-MS/MS analysis. In addition to (+)-cis,trans-ABA and its glucosyl ester, a new abscisic acid conjugate, ABA-L-glutamate, was isolated by IAC and identified by tandem mass spectrometry in pea (Pisum sativum L.), Lepidium sativum L. and wheat (Triticum aestivum L.) seedlings. However, it was not found in 10-day-old seedlings of Arabidopsis thaliana or water-stressed tobacco (Nicotiana tabacum L.) leaves. Here, the identification of an ABA conjugate with glutamic acid in plants is described for the first time.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {The effect of Polygonum hyrcanicum Rech. f. hydroalcoholic extract on oxidative stress and nephropathy in alloxan-induced diabetic mice},

author = {Aday Amirbekov and Stanislava Vrchovecka and Jakub Riha and Ivan Petrik and David Friedecky and Ondrej Novak and Miroslav Cernik and Pavel Hrabak and Alena Sevcu},

url = {10.1038/s41598-024-69220-x},

doi = {10.1038/s41598-024-69220-x},

issn = {2045-2322},

year  = {2024},

date = {2024-08-05},

journal = {SCIENTIFIC REPORTS},

volume = {14},

issue = {1},

abstract = {Diabetic nephropathy, characterized by inflammation and oxidative stress, poses a management challenge. This study investigates the effect of Polygonum hyrcanicum extract on diabetic nephropathy in alloxan-induced diabetic mice. In this experimental animal study, the P. hyrcanicum extract was prepared using continuous macerations. Thirty male Albino mice, divided into five groups, were induced with alloxan-induced diabetes. They received intraperitoneal injections of the plant extract (100 and 200 mg/kg) and metformin (300 mg/kg) for four weeks. Kidney and blood samples were collected to assess protein carbonyl, glutathione, lipid peroxidation, TNF-alpha and IL-6 levels. The amount of total flavonoid and phenolic content in the hydroalcoholic extract of P. hyrcanicum were 7.5 +/- 0.3 mg of quercetin and 88.2 +/- 1.3 mg gallic acid per gram of extract respectively. The antioxidant activity level of the hydroalcoholic extract was determined to be 1.78 +/- 0.51 mM equivalent per gram of extract. Alloxan administration resulted in a significant reduction in glutathione levels and a significant increase in protein carbonyl, lipid peroxidation, TNF-alpha, and IL-6 levels. Hydroalcoholic extract of P. hyrcanicum effectively reduced oxidative stress markers and inflammatory cytokines (TNF-alpha, IL-6), indicating its potential in mitigating diabetic nephropathy. However, no significant difference in efficacy was observed between the 100 mg/kg and 200 mg/kg doses in terms of reducing these toxicities.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Systemic strategies for cytokinin biosynthesis and catabolism in Arabidopsis roots and leaves under prolonged ammonium nutrition},

author = {Kacper Dziewit and Petra Amakorova and Ondrej Novak and Bozena Szal and Anna Podgorska},

url = {10.1016/j.plaphy.2024.108858

},

doi = {10.1016/j.plaphy.2024.108858},

issn = {0981-9428},

year  = {2024},

date = {2024-08-01},

journal = {PLANT PHYSIOLOGY AND BIOCHEMISTRY},

volume = {213},

abstract = {Cytokinins are growth -regulating plant hormones that are considered to adjust plant development under environmental stresses. During sole ammonium nutrition, a condition known to induce growth retardation of plants, altered cytokinin content can contribute to the characteristic ammonium toxicity syndrome. To understand the metabolic changes in cytokinin pools, cytokinin biosynthesis and degradation were analyzed in the leaves and roots of mature Arabidopsis plants. We found that in leaves of ammonium -grown plants, despite induction of biosynthesis on the expression level, there was no active cytokinin build-up because they were effectively routed toward their downstream catabolites. In roots, cytokinin conjugation was also induced, together with low expression of major synthetic enzymes, resulting in a decreased content of the trans -zeatin form under ammonium conditions. Based on these results, we hypothesized that in leaves and roots, cytokinin turnover is the major regulator of the cytokinin pool and does not allow active cytokinins to accumulate. A potent negative -regulator of root development is trans -zeatin, therefore its low level in mature root tissues of ammonium -grown plants may be responsible for occurrence of a wide root system. Additionally, specific cytokinin enhancement in apical root tips may evoke a short root phenotype in plants under ammonium conditions. The ability to flexibly regulate cytokinin metabolism and distribution in root and shoot tissues can contribute to adjusting plant development in response to ammonium stress.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Karrikinolide1 (KAR1), a Bioactive Compound from Smoke, Improves the Germination of Morphologically Dormant Apium graveolens L. Seeds by Reducing Indole-3-Acetic Acid (IAA) Levels},

author = {Shubhpriya Gupta and Jakub Hrdlicka and Manoj Kulkarni and Ivana Dolezalova and Ales Pencik and Johannes Van Staden and Ondrej Novak and Karel Dolezal},

url = {10.3390/plants13152096

},

doi = {10.3390/plants13152096},

issn = {2223-7747},

year  = {2024},

date = {2024-08-01},

journal = {PLANTS-BASEL},

volume = {13},

issue = {15},

abstract = {Smoke-water (SW) and Karrikinolide1 (KAR(1)) release dormancy and improve seed germination in many plant species. Therefore, we tested SW (1:2500 v/v) and KAR(1) (10(-7) M) to break the morphological dormancy of celery cultivar (Apium graveolens L.). In the first trial, seeds were subjected to a 21-day incubation period at 20 degrees C with SW and KAR(1) applied as single treatments. KAR(1) showed significantly improved germination (30.7%) as compared to SW (17.2%) and a water control (14.7%). In seed soaking experiments, SW, KAR(1), and gibberellic acid (GA(3)) treatments showed higher germination percentages than the water control after 3 and 6 h of soaking. However, prolonged soaking (12 h) reduced germination percentages for all treatments, indicating a detrimental effect. Analysis of KAR(1) content dynamics in 7-day- and 21-day-old celery seeds indicated its prolonged effects on germination and dormancy alleviation. Phytohormones, including auxins in 7-day-old and cytokinins in 7-day- and 21-day-old celery seedlings, along with their precursors and metabolites, were analyzed using ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) after treatment with KAR(1) and SW. The analysis of auxin levels in 7-day-old seeds revealed a negative correlation between seed germination and auxin (indole-3-acetic acid, IAA) content. Notably, it was found that KAR(1)-treated seeds significantly reduced IAA levels in all treatments. SW and KAR(1) did not significantly affect cytokinin levels during celery germination except for N6-Isopentenyladenine. Hence, further research is needed to understand their precise role in celery seed germination. This work will improve our understanding of the role of bioactive compounds from plant-derived smoke and how they regulate hormonal responses and improve germination efficiency in celery.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{,

title = {Spatio-temporal plant hormonomics: from tissue to subcellular resolution},

author = {Ivan Petrik and Pavel Hladik and Chao Zhang and Ales Pencik and Ondrej Novak},

url = {10.1093/jxb/erae267},

doi = {10.1093/jxb/erae267},

issn = {0022-0957},

year  = {2024},

date = {2024-07-22},

urldate = {2024-01-01},

journal = {JOURNAL OF EXPERIMENTAL BOTANY},

volume = {75},

number = {5295},

issue = {17},

pages = {5311},

abstract = {Due to technological advances in mass spectrometry, significant progress has been achieved recently in plant hormone research. Nowadays, plant hormonomics is well established as a fully integrated scientific field focused on the analysis of phytohormones, mainly on their isolation, identification, and spatiotemporal quantification in plants. This review represents a comprehensive meta-study of the advances in the phytohormone analysis by mass spectrometry over the past decade. To address current trends and future perspectives, Web of Science data were systematically collected and key features such as mass spectrometry-based analyses were evaluated using multivariate data analysis methods. Our findings showed that plant hormonomics is currently divided into targeted and untargeted approaches. Both aim to miniaturize the sample, allowing high-resolution quantification to be covered in plant organs as well as subcellular compartments. Therefore, we can study plant hormone biosynthesis, metabolism, and signalling at a spatio-temporal resolution. Moreover, this trend has recently been accelerated by technological advances such as fluorescence-activated cell sorting or mass spectrometry imaging. A comprehensive meta-study was conducted to reveals research trends in plant hormone determination using targeted and untargeted metabolomic approaches published in the last decade.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}