Publikace

@article{nokey,

title = {Hormonal profiles in dormant turions of 22 aquatic plant species: do they reflect functional or taxonomic traits?},

author = {Lubomir Adamec and Lenka Plackova and Martin Bitomsky and Karel Doležal},

url = {10.1093/aob/mcae059},

doi = {10.1093/aob/mcae059},

issn = {0305-7364},

year  = {2024},

date = {2024-05-07},

urldate = {2024-05-07},

journal = {ANNALS OF BOTANY},

volume = {134},

issue = {2},

pages = {219-232},

abstract = {Background and Aims Turions are vegetative, dormant overwintering organs formed in aquatic plants in response to unfavourable ecological conditions. Contents of cytokinin (CK), auxin metabolites and abscisic acid (ABA) as main growth and development regulators were compared in innately dormant autumnal turions of 22 aquatic plant species of different functional ecological or taxonomic groups with those in non-dormant winter apices in three aquatic species and with those in spring turions of four species after their overwintering.Methods The hormones were analysed in miniature turion samples using ultraperformance liquid chromatography coupled with triple quadrupole mass spectrometry.Key Results In innately dormant turions, the total contents of each of the four main CK types, biologically active forms and total CKs differed by two to three orders of magnitude across 22 species; the proportion of active CK forms was 0.18-67 %. Similarly, the content of four auxin forms was extremely variable and the IAA proportion as the active form was 0.014-99 %. The ABA content varied from almost zero to 54 mu mol kg-1 dry weight and after overwintering it usually significantly decreased. Of all functional traits studied, hormone profiles depended most on the place of turion sprouting (surface vs bottom) and we suggest that this trait is crucial for turion ecophysiology.Conclusions The key role of ABA in regulating turion dormancy was confirmed. However, the highly variable pattern of the ABA content in innately dormant and in overwintered turions indicates that the hormonal mechanism regulating the innate dormancy and its breaking in turions is not uniform within aquatic plants.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Strigolactones promote flowering by inducing the miR319- LA- SFT module in tomato},

author = {Ivan Visentin and Leticia Frizzo Ferigolo and Giulia Russo and Paolo Korwin Krukowski and Caterina Capezzali and Danuse Tarkowska and Francesco Gresta and Eleonora Deva and Fabio Tebaldi Silveira Nogueira and Andrea Schubert and Francesca Cardinale},

url = {10.1073/pnas.2316371121

},

doi = {10.1073/pnas.2316371121},

isbn = {0027-8424},

year  = {2024},

date = {2024-05-07},

urldate = {2024-05-07},

journal = {PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA},

volume = {121},

issue = {19},

pages = {1-10},

abstract = {Strigolactones are a class of phytohormones with various functions in plant development, stress responses, and in the interaction with (micro)organisms in the rhizosphere. While their effects on vegetative development are well studied, little is known about their role in reproduction. We investigated the effects of genetic and chemical modification of strigolactone levels on the timing and intensity of flowering in tomato ( Solanum lycopersicum L.) and the molecular mechanisms underlying such effects. Results showed that strigolactone levels in the shoot, whether endogenous or exogenous, correlate inversely with the time of anthesis and directly with the number of flowers and the transcript levels of the florigen - encoding gene SINGLE FLOWER TRUSS ( SFT ) in the leaves. Transcript quantifications coupled with metabolite analyses demonstrated that strigolactones promote flowering in tomato by inducing the activation of the microRNA319 - LANCEOLATE module in leaves. This, in turn, decreases gibberellin content and increases the transcription of SFT . Several other floral markers and morpho - anatomical features of developmental progression are induced in the apical meristems upon treatment with strigolactones, affecting floral transition and, more markedly, flower development. Thus, strigolactones promote meristem maturation and flower development via the induction of SFT both before and after floral transition, and their effects are blocked in plants expressing a miR319 - resistant version of LANCEOLATE . Our study positions strigolactones in the context of the flowering regulation network in a model crop species.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {The GH3 Protein from the Clubroot Pathogen Plasmodiophora brassicae Causes Hormone-Related Phenotypes in Arabidopsis thaliana},

author = {Ana Smolko and Sabine Juelke and Freia Benade and Jitka Siroka and Ales Pencik and Tamara Vuk and Natasa Bauer and Branka Salopek-Sondi and Jutta Ludwig-Mueller},

url = {10.1007/s00344-024-11300-3

},

doi = {10.1007/s00344-024-11300-3},

issn = {0721-7595},

year  = {2024},

date = {2024-05-06},

journal = {JOURNAL OF PLANT GROWTH REGULATION},

volume = {43},

issue = {8},

pages = {2715-2738},

abstract = {In the genome of the obligate biotrophic protist Plasmodiophora brassicae, which causes clubroot disease in the Brassicaceae family, a single gene was identified with homology to the group of GH3 proteins synthesizing amino acid conjugates with carboxylic acids. In comparison to plant members the P. brassicae GH3 protein seems to be very promiscuous with respect to the substrates converted. In vitro assays showed conversion of three different auxins (indole-3-acetic, IAA; indole-propionic, IPA; and indole-3-butyric acids, IBA), jasmonic acid (JA) and also 12-oxo-phytodienoic acid (OPDA). Auxin and Methyl-JA responsive elements were found in the promoter sequence of PbGH3. For further analysis Arabidopsis thaliana was transformed with PbGH3. Seedlings with confirmed constitutive PbGH3 protein production were grown to adult plants that were shown to produce fewer inflorescences per plant. Growth on 10 mu M IAA or JA levels also altered the phenotype of the seedlings, but not so much between wild types and transgenic lines. The hormone profiles in seedlings and adult plants were examined for IAA, JA, OPDA, and respective conjugates. Transgenic seedlings displayed changes that could be partially attributed to the overexpression of a GH3 gene like lower levels of free IAA and the JA precursor cis-OPDA under control conditions, and higher levels of some auxin conjugates accumulated after IAA treatment compared to the corresponding wild types. However, the hormone patterns in adult plants were more complex and varied based on different tissue types analyzed. After inoculation with P. brassicae the transgenic plants did not show any changes in infection rate and disease index, but had higher shoot and lower root weight in infected plants compared to controls. Based on the above findings we discuss a role for PbGH3 during pathogenesis.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {In situ seasonal patterns of root auxin concentrations and meristem length in an arctic sedge},

author = {Gesche Blume-Werry and Philipp Semenchuk and Karin Ljung and Ann Milbau and Ondrej Novak and Johan Olofsson and Federica Brunoni },

url = {10.1111/nph.19616

},

doi = {10.1111/nph.19616},

issn = {0028-646X},

year  = {2024},

date = {2024-05-01},

journal = {NEW PHYTOLOGIST},

volume = {242},

issue = {3},

pages = {988-999},

abstract = {Seasonal dynamics of root growth play an important role in large-scale ecosystem processes; they are largely governed by growth regulatory compounds and influenced by environmental conditions. Yet, our knowledge about physiological drivers of root growth is mostly limited to laboratory-based studies on model plant species. We sampled root tips of Eriophorum vaginatum and analyzed their auxin concentrations and meristem lengths biweekly over a growing season in situ in a subarctic peatland, both in surface soil and at the permafrost thawfront. Auxin concentrations were almost five times higher in surface than in thawfront soils and increased over the season, especially at the thawfront. Surprisingly, meristem length showed an opposite pattern and was almost double in thawfront compared with surface soils. Meristem length increased from peak to late season in the surface soils but decreased at the thawfront. Our study of in situ seasonal dynamics in root physiological parameters illustrates the potential for physiological methods to be applied in ecological studies and emphasizes the importance of in situ measurements. The strong effect of root location and the unexpected opposite patterns of meristem length and auxin concentrations likely show that auxin actively governs root growth to ensure a high potential for nutrient uptake at the thawfront.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Oxime derivatives of betulonic acid and platanic acid as novel cytotoxic or antiviral agents},

author = {Lucie Cerna and Uladzimir Bildziukevich and Lucie Rarova and Jana Trylcova and David Saman and Jan Weber and Petra Lovecka and Zdenek Wimmer},

url = {10.1039/d4re00032c

},

doi = {10.1039/d4re00032c},

issn = {2058-9883},

year  = {2024},

date = {2024-04-30},

journal = {REACTION CHEMISTRY & ENGINEERING},

volume = {9},

issue = {5},

pages = {1087-1095},

abstract = {Less frequently studied plant triterpenoids betulonic acid and platanic acid were selected to design, synthesize and investigate their oxime derivatives as novel and potentially effective cytotoxic and/or antiviral agents. The target compounds were subjected to cytotoxicity screening tests in several human cancer cell lines. Several of them displayed cytotoxicity against T-lymphoblastic leukemia (CCRF-CEM), breast adenocarcinoma (MCF7), malignant melanoma (G-361) and cervical cancer (HeLa) cell lines. Betulonic acid oxime (3) displayed cytotoxicity against CCRF-CEM (IC50 = 18.9 +/- 1.1 mu M; SI = 2.4) and G-361 (IC50 = 21.3 +/- 2.8 mu M; SI = 2.2) cancer cell lines. The benzyl ester of platanic acid oxime (17) displayed an anti-HIV-1 effect (EC50 = 3.2 +/- 0.43 mu M; SI > 31) and no anti-HSV-1 effect (EC50 > 100 mu M), while platanic acid oxime (15) showed lower effects, EC50 = 36 +/- 4.0 mu M (HIV-1) and EC50 = 48 +/- 6.0 mu M (HSV-1), respectively. Compound 17 showed high selectivity in antiviral effects, being effective in HIV-1 and inactive in HSV-1. A side product 18 showed antiviral activity with EC50 = 15 +/- 1.3 mu M (HIV-1) and EC50 = 12 +/- 0.21 mu M (HSV-1), while another side product 19 was cytotoxic to HeLa (IC50 = 24.5 +/- 1.8 mu M).

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Simultaneous Determination of Selected Steroids with Neuroactive Effects in Human Serum by Ultrahigh-Performance Liquid Chromatography-Tandem Mass Spectrometry},

author = {Michal Kaleta and Jana Oklestkova and Katerina Klicova and Miroslav Kvasnica and Dorota Konickova and Katerina Mensikova and Miroslav Strnad and Ondrej Novak},

url = {10.1021/acschemneuro.3c00824

},

doi = {10.1021/acschemneuro.3c00824},

issn = {1948-7193},

year  = {2024},

date = {2024-04-24},

journal = {ACS CHEMICAL NEUROSCIENCE},

volume = {15},

issue = {10},

pages = {1990-2005},

abstract = {Neuroactive steroids are a group of steroid molecules that are involved in the regulation of functions of the nervous system. The nervous system is not only the site of their action, but their biosynthesis can also occur there. Neuroactive steroid levels depend not only on the physiological state of an individual (person's sex, age, diurnal variation, etc.), but they are also affected by various pathological processes in the nervous system (some neurological and psychiatric diseases or injuries), and new knowledge can be gained by monitoring these processes. The aim of our research was to develop and validate a comprehensive method for the simultaneous determination of selected steroids with neuroactive effects in human serum. The developed method enables high throughput and a sensitive quantitative analysis of nine neuroactive steroid substances (pregnenolone, progesterone, 5 alpha-dihydroprogesterone, allopregnanolone, testosterone, 5 alpha-dihydrotestosterone, androstenedione, dehydroepiandrosterone, and epiandrosterone) in 150 mu L of human serum by ultrahigh-performance liquid chromatography with tandem mass spectrometry. The correlation coefficients above 0.999 indicated that the developed analytical procedure was linear in the range of 0.90 nmol/L to 28.46 mu mol/L in human serum. The accuracy and precision of the method for all analytes ranged from 83 to 118% and from 0.9 to 14.1%, respectively. This described method could contribute to a deeper understanding of the pathophysiology of various diseases. Similarly, it can also be helpful in the search for new biomarkers and diagnostic options or therapeutic approaches.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Analytical Methods for Brassinosteroid Analysis: Recent Advances and Applications},

author = {Jana Oklestkova and Miroslav Kvasnica and Miroslav Strnad},

url = {10.1093/pcp/pcae038

},

doi = {10.1093/pcp/pcae038},

issn = {0032-0781},

year  = {2024},

date = {2024-04-15},

urldate = {2024-04-15},

journal = {PLANT AND CELL PHYSIOLOGY},

volume = {65},

number = {1618},

issue = {10},

pages = {1626},

abstract = {Brassinosteroids (BRs) are plant steroidal hormones that play crucial roles in plant growth and development. Accurate quantification of BRs in plant tissues is essential for understanding their biological functions. This study presents a comprehensive overview of the latest methods used for the quantification of BRs in plants. We discuss the principles, advantages and limitations of various analytical techniques, including immunoassays, gas chromatography-mass spectrometry and liquid chromatography-tandem mass spectrometry that are used for the detection and quantification of BRs from complex plant matrixes. We also explore the use of isotopically labeled internal standards to improve the accuracy and reliability of BR quantification.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {The dimorphic diaspore model Aethionema arabicum (Brassicaceae): Distinct molecular and morphological control of responses to parental and germination temperatures},

author = {Jake O. Chandler and Per K. I. Wilhelmsson and Noe Fernandez-Pozo and Kai Graeber and Waheed Arshad and Marta Perez and Tina Steinbrecher and Kristian K. Ullrich and Thu-Phuong Nguyen and Zsuzsanna Merai and Klaus Mummenhoff and Guenter Theissen and Miroslav Strnad and Ortrun Mittelsten Scheid and M. Eric Schranz and Ivan Petrik and Danuse Tarkowska and Ondrej Novak and Stefan A. Rensing and Gerhard Leubner-Metzger},

url = {10.1093/plcell/koae085

},

doi = {10.1093/plcell/koae085},

issn = {1040-4651},

year  = {2024},

date = {2024-04-15},

journal = {PLANT CELL},

volume = {35},

issue = {7},

pages = {2465-2490},

abstract = {Plants in habitats with unpredictable conditions often have diversified bet-hedging strategies that ensure fitness over a wider range of variable environmental factors. A striking example is the diaspore (seed and fruit) heteromorphism that evolved to maximize species survival in Aethionema arabicum (Brassicaceae) in which external and endogenous triggers allow the production of two distinct diaspores on the same plant. Using this dimorphic diaspore model, we identified contrasting molecular, biophysical, and ecophysiological mechanisms in the germination responses to different temperatures of the mucilaginous seeds (M+ seed morphs), the dispersed indehiscent fruits (IND fruit morphs), and the bare non-mucilaginous M- seeds obtained by pericarp (fruit coat) removal from IND fruits. Large-scale comparative transcriptome and hormone analyses of M+ seeds, IND fruits, and M- seeds provided comprehensive datasets for their distinct thermal responses. Morph-specific differences in co-expressed gene modules in seeds, as well as in seed and pericarp hormone contents, identified a role of the IND pericarp in imposing coat dormancy by generating hypoxia affecting abscisic acid (ABA) sensitivity. This involved expression of morph-specific transcription factors, hypoxia response, and cell wall remodeling genes, as well as altered ABA metabolism, transport, and signaling. Parental temperature affected ABA contents and ABA-related gene expression and altered IND pericarp biomechanical properties. Elucidating the molecular framework underlying the diaspore heteromorphism can provide insight into developmental responses to globally changing temperatures. Aethionema arabicum is an outstanding model to understand the plastic developmental and environmental control of the dimorphic seed/fruit bet-hedging trait relevant to adaptation to abiotic stress.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{WOS:001107348800001,

title = {Endophyte Inoculation Enhances Growth, Secondary Metabolites and  Biological Activity of \textit{Endostemon obtusifolius} Grown Under  Drought Stress},

author = {Abdulazeez A. Ogbe and Shubhpriya Gupta and Wendy A. Stirk and Jeffrey F. Finnie and Johannes Staden},

url = {10.1007/s00344-023-11167-w},

doi = {10.1007/s00344-023-11167-w},

issn = {0721-7595},

year  = {2024},

date = {2024-04-01},

urldate = {2024-04-01},

journal = {JOURNAL OF PLANT GROWTH REGULATION},

volume = {43},

number = {4},

pages = {1103-1117},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{WOS:001084334300001,

title = {New fluorescent auxin derivatives: anti-auxin activity and accumulation  patterns in \textit{Arabidopsis thaliana}},

author = {Kristyna Bieleszova and Pavel Hladik and Martin Kubala and Richard Napier and Federica Brunoni and Zuzana Gelova and Lukas Fiedler and Ivan Kulich and Miroslav Strnad and Karel Dolezal and Ondrej Novak and Jiri Friml and Asta Zukauskaite},

url = {10.1007/s10725-023-01083-0},

doi = {10.1007/s10725-023-01083-0},

issn = {0167-6903},

year  = {2024},

date = {2024-04-01},

urldate = {2024-04-01},

journal = {PLANT GROWTH REGULATION},

volume = {102},

number = {3},

pages = {589-602},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {New Inhibitors of Bcr-Abl Based on 2,6,9-Trisubstituted Purine Scaffold Elicit Cytotoxicity in Chronic Myeloid Leukemia-Derived Cell Lines Sensitive and Resistant to TKIs},

author = {Thalia Delgado and Denisa Vesela and Hana Dostalova and Vladimir Krystof and Veronika Vojackova and Radek Jorda and Alejandro Castro and Jeanluc Bertrand and Gildardo Rivera and Mario Faundez and Miroslav Strnad and Christian Espinosa-Bustos and Cristian O. Salas},

url = {10.3390/pharmaceutics16050649

},

doi = {10.3390/pharmaceutics16050649},

year  = {2024},

date = {2024-04-01},

journal = {PHARMACEUTICS},

volume = {16},

issue = {5},

abstract = {Bcr-Abl is an oncoprotein with aberrant tyrosine kinase activity involved in the progression of chronic myeloid leukemia (CML) and has been targeted by inhibitors such as imatinib and nilotinib. However, despite their efficacy in the treatment of CML, a mechanism of resistance to these drugs associated with mutations in the kinase region has emerged. Therefore, in this work, we report the synthesis of 14 new 2,6,9-trisubstituted purines designed from our previous Bcr-Abl inhibitors. Here, we highlight 11b, which showed higher potency against Bcr-Abl (IC50 = 0.015 mu M) than imatinib and nilotinib and exerted the most potent antiproliferative properties on three CML cells harboring the Bcr-Abl rearrangement (GI(50) = 0.7-1.3 mu M). In addition, these purines were able to inhibit the growth of KCL22 cell lines expressing Bcr-Abl(T315I), Bcr-Abl(E255K), and Bcr-Abl(Y253H) point mutants in micromolar concentrations. Imatinib and nilotinib were ineffective in inhibiting the growth of KCL22 cells expressing Bcr-Abl(T315I) (GI(50) > 20 mu M) compared to 11b-f (GI(50) = 6.4-11.5 mu M). Molecular docking studies explained the structure-activity relationship of these purines in Bcr-Abl(WT) and Bcr-Abl(T315I). Finally, cell cycle cytometry assays and immunodetection showed that 11b arrested the cells in G1 phase, and that 11b downregulated the protein levels downstream of Bcr-Abl in these cells.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Seed priming with gas plasma-activated water in Ethiopia's orphan crop tef (Eragrostis tef)},

author = {Lena M. M. Fatelnig and Solomon Chanyalew and Mahilet Tadesse and Worku Kebede and Nigusu Hussein and Felipe Iza and Zerihun Tadele and Gerhard Leubner-Metzger and Tina Steinbrecher},

url = {10.1007/s00425-024-04359-5

},

doi = {10.1007/s00425-024-04359-5},

issn = {0032-0935},

year  = {2024},

date = {2024-04-01},

urldate = {2024-07-01},

journal = {PLANTA},

volume = {259},

issue = {4},

abstract = {Main conclusionSeed priming with gas plasma-activated water results in an increased ageing resilience in Eragrostis tef grains compared to a conventional hydropriming protocol. AbstractTef (Eragrostis tef) is a cereal grass and a major staple crop of Ethiopia and Eritrea. Despite its significant importance in terms of production, consumption, and cash crop value, tef has been understudied and its productivity is low. In this study, tef grains have undergone different priming treatments to enhance seed vigour and seedling performance. A conventional hydropriming and a novel additive priming technology with gas plasma-activated water (GPAW) have been used and tef grains were then subjected to germination performance assays and accelerated ageing. Tef priming increases the germination speed and vigour of the grains. Priming with GPAW retained the seed storage potential after ageing, therefore, presenting an innovative environmental-friendly seed technology with the prospect to address variable weather conditions and ultimately food insecurity. Seed technology opens new possibilities to increase productivity of tef crop farming to achieve a secure and resilient tef food system and economic growth in Ethiopia by sustainable intensification of agriculture beyond breeding.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Foliar smoke-water application improves growth and changes the leaf mineral composition of radish, spinach and turnip seedlings},

author = {Shubhpriya Gupta and Manoj Kulkarni and Ivana Dolezalova and Ondrej Novak and Johannes Van Staden and Karel Dolezal},

url = {10.1016/j.sajb.2024.02.045

},

doi = {10.1016/j.sajb.2024.02.045},

issn = {0254-6299},

year  = {2024},

date = {2024-04-01},

journal = {SOUTH AFRICAN JOURNAL OF BOTANY},

volume = {167},

pages = {527-534},

abstract = {Smoke -water, a smoke -saturated solution derived from the smoke generated by burning plant materials, promotes seed germination and growth of various plant species. There are many reported studies where seeds are treated and soils are drenched with smoke -water to explore the beneficial effects of smoke. However, little information is available on the influence of foliar spraying of smoke -water on the seedlings of commercial vegetable crops for better growth prospects. This study, therefore, investigated the effects of standardized smoke -water (1:2500 v/v) foliar application on the seedling growth of radish (Raphanus sativus L.), spinach (Spinacia oleracea L.) and turnip (Brassica rapa L.), and determined the mineral elemental composition of leaves. Smoke -water foliar treatment significantly enhanced the length and weight of seedlings compared to the seedlings sprayed with water (control). Mineral elements, potassium and zinc, were increased notably in the leaves of all smoke -water foliar-treated seedlings. This can be attributed to increased photosynthetic activity that resulted in longer and heavier seedlings. The results of the present study provide an opportunity to incorporate the use of smoke -water for raising vigorous and healthier vegetable seedlings for better yield. Moreover, it can minimize the input of conventional chemical sprays that are detrimental to the environment. (c) 2024 SAAB. Published by Elsevier B.V. All rights reserved.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Isolation and Bioactivity Evaluation of Sesquiterpenes from an Alcyonarian of the Genus Lemnalia from the Saudi Arabian Red Sea},

author = {Aikaterini Koutsaviti and Marie Kvasnicova and Gabriel Gonzalez and Tereza Stenclova and Susana Agusti and Carlos M. Duarte and Lucie Rarova and Miroslav Strnad and Vassilios Roussis and Efstathia Ioannou},

url = {10.1002/cbdv.202400235

},

doi = {10.1002/cbdv.202400235},

issn = {1612-1872},

year  = {2024},

date = {2024-04-01},

journal = {CHEMISTRY & BIODIVERSITY},

volume = {21},

issue = {4},

abstract = {Over the last decades, soft corals have been proven a rich source of biologically active compounds, featuring a wide range of chemical structures. Herein, we investigated the chemistry of an alcyonarian of the genus Lemnalia (Neptheidae), specimens of which were collected from the coral reefs near Al Lith, on the south-west coast of Saudi Arabia. A series of chromatographic separations led to the isolation of 31 sesquiterpenes, featuring mainly the nardosinane and neolemnane carbon skeletons, among which three (13, 14 and 28) are new natural products. The metabolites isolated in sufficient amounts were evaluated in vitro in human tumor and non-cancerous cell lines for a number of biological activities, including their cytotoxic, anti-inflammatory, anti-angiogenic, and neuroprotective activities, as well as for their effect on androgen receptor (AR)-regulated transcription. Among the tested metabolites, compound 12 showed comparable neuroprotective activity to the positive control N-acetylcysteine, albeit at a 10-fold lower concentration.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Dairy products authentication with biomarkers: A comprehensive critical review},

author = {Eman Shawky and Lutfun Nahar and Sarah M. Nassief and Satyajit D. Sarker and Reham S. Ibrahim},

url = {10.1016/j.tifs.2024.104445

},

doi = {10.1016/j.tifs.2024.104445},

issn = {0924-2244},

year  = {2024},

date = {2024-03-18},

journal = {TRENDS IN FOOD SCIENCE & TECHNOLOGY},

volume = {147},

abstract = {Background: This review highlights the critical need for innovative biomarkers in authenticating milk and its derivatives to ensure quality, authenticity and safety in the food industry. The limitations of traditional methods in detecting sophisticated adulteration techniques underscore the urgency for advanced solutions. Scope and approach: The review explores biomarker discovery as a promising avenue, utilizing specific molecular indicators to provide credible evidence about the origin, genuineness, and overall quality of milk and its products. It critically examines the challenges and shortcomings of existing authentication methods, emphasizing the necessity for novel biomarkers. The study encompasses various strategies in biomarker discovery, including genomics, proteomics, metabolomics, and other -omics approaches, contextualized within high-throughput technologies. Key findings and conclusions: Assessing potential biomarker sources within milk and dairy, such as intrinsic components, microbial communities, processing markers, and environmental factors, the review rigorously evaluates their applicability, sensitivity, specificity, and practical utility in ensuring authenticity and safety. A critical analysis addresses challenges related to biomarker validation, including standardization, reproducibility, and integration with existing authentication methods. The incorporation of these novel biomarkers holds the promise of reinforcing the authentication process, providing heightened protection against fraudulent practices and strengthening consumer trust and satisfaction within the milk and dairy sector.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Profiling of 1-aminocyclopropane-1-carboxylic acid and selected phytohormones in Arabidopsis using liquid chromatography-tandem mass spectrometry},

author = {Michal Karady and Pavel Hladik and Katerina Cermanova and Petra Jiroutova and Ioanna Antoniadi and Ruben Casanova-Saez and Karin Ljung and Ondrej Novak},

url = {10.1186/s13007-024-01165-8

},

doi = {10.1186/s13007-024-01165-8},

issn = {1746-4811},

year  = {2024},

date = {2024-03-16},

journal = {PLANT METHODS},

volume = {20},

issue = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Aethionema arabicum dimorphic seed trait resetting during transition to seedlings},

author = {Waheed Arshad and Tina Steinbrecher and Per K. I. Wilhelmsson and Noe Fernandez-Pozo and Marta Perez and Zsuzsanna Merai and Stefan A. Rensing and Jake O. Chandler and Gerhard Leubner-Metzger},

url = {10.3389/fpls.2024.1358312},

doi = {10.3389/fpls.2024.1358312},

issn = {1664-462X},

year  = {2024},

date = {2024-03-08},

journal = {FRONTIERS IN PLANT SCIENCE},

volume = {15},

abstract = {The transition from germinating seeds to emerging seedlings is one of the most vulnerable plant life cycle stages. Heteromorphic diaspores (seed and fruit dispersal units) are an adaptive bet-hedging strategy to cope with spatiotemporally variable environments. While the roles and mechanisms of seedling traits have been studied in monomorphic species, which produce one type of diaspore, very little is known about seedlings in heteromorphic species. Using the dimorphic diaspore model Aethionema arabicum (Brassicaceae), we identified contrasting mechanisms in the germination responses to different temperatures of the mucilaginous seeds (M+ seed morphs), the dispersed indehiscent fruits (IND fruit morphs), and the bare non-mucilaginous M- seeds obtained from IND fruits by pericarp (fruit coat) removal. What follows the completion of germination is the pre-emergence seedling growth phase, which we investigated by comparative growth assays of early seedlings derived from the M+ seeds, bare M- seeds, and IND fruits. The dimorphic seedlings derived from M+ and M- seeds did not differ in their responses to ambient temperature and water potential. The phenotype of seedlings derived from IND fruits differed in that they had bent hypocotyls and their shoot and root growth was slower, but the biomechanical hypocotyl properties of 15-day-old seedlings did not differ between seedlings derived from germinated M+ seeds, M- seeds, or IND fruits. Comparison of the transcriptomes of the natural dimorphic diaspores, M+ seeds and IND fruits, identified 2,682 differentially expressed genes (DEGs) during late germination. During the subsequent 3 days of seedling pre-emergence growth, the number of DEGs was reduced 10-fold to 277 root DEGs and 16-fold to 164 shoot DEGs. Among the DEGs in early seedlings were hormonal regulators, in particular for auxin, ethylene, and gibberellins. Furthermore, DEGs were identified for water and ion transporters, nitrate transporter and assimilation enzymes, and cell wall remodeling protein genes encoding enzymes targeting xyloglucan and pectin. We conclude that the transcriptomes of seedlings derived from the dimorphic diaspores, M+ seeds and IND fruits, undergo transcriptional resetting during the post-germination pre-emergence growth transition phase from germinated diaspores to growing seedlings.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{WOS:001113197000001,

title = {Plant nucleoside N-ribohydrolases: riboside binding and role in nitrogen 

 storage mobilization},

author = {Eva Luptakova and Armelle Vigouroux and Radka Koncitikova and Martina Kopecna and David Zalabak and Ondrej Novak and Sara Salcedo Sarmiento and Sanja Cavar Zeljkovic and David Jaroslav Kopecny and Klaus Schwartzenberg and Miroslav Strnad and Lukas Spichal and Nuria De Diego and David Kopecny and Solange Morera},

doi = {10.1111/tpj.16572},

issn = {0960-7412},

year  = {2024},

date = {2024-03-01},

journal = {PLANT JOURNAL},

volume = {117},

number = {5},

pages = {1432-1452},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Gold(I) N-heterocyclic carbene (NHC) complexes containing 6-mercaptopurine derivatives and their in vitro anticancer and anti-inflammatory effects},

author = {Zdenek Travnicek and Jan Vanco and Michal Cajan and Jan Belza and Igor Popa and Jan Hosek and Rene Lenobel and Zdenek Dvorak},

url = {10.1002/aoc.7401

},

doi = {10.1002/aoc.7401},

issn = {0268-2605},

year  = {2024},

date = {2024-02-22},

journal = {APPLIED ORGANOMETALLIC CHEMISTRY},

volume = {38},

issue = {4},

abstract = {A series of eight N-heterocyclic carbenes (NHC) gold(I) complexes, involving 1,3-bis(2,6-diisopropylphenyl)imidazol-2-ylidene (iPr) ligand in combination with 6-mercaptopurine derivatives (HL1-8), has been prepared and thoroughly characterized, including elemental analysis, mass spectrometry, infrared and multinuclear NMR spectroscopy, and single crystal X-ray analysis. The complexes, showing general composition of [Au (iPr)(Ln)] 1-8, were evaluated for their in vitro cytotoxicity against four human cancer cell lines including A2780 (ovarian) and A2780R (ovarian Cisplatin resistant), PC3 (prostate) and MCF-7 (breast), and normal human MRC-5 cells (lung fibroblasts). The complexes revealed significant cytotoxicity, with the best IC50 values approximate to 3.4-6.4 mu M against A2780 and reasonable selectivity. Cellular effects of the selected complexes on the A2780 cells were evaluated using various flow cytometry assays. Complexes 1, 3, and 4 showed a strong pro-apoptotic effect and a strong effect on the loss of mitochondrial membrane potential. These findings indicate that their major mechanism of action is based on the collapse of the mitochondrial metabolism and activation of the intrinsic signaling pathway of apoptosis, consequently resulting in cell death. The complexes 1-8 revealed only negligible effect on the production of inflammatory-related cytokine (TNF-alpha), as well as the activation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kappa B) or peroxisome proliferator-activated receptor gamma (PPAR gamma). Moreover, the shotgun proteomic analysis was performed, and the obtained results suggest that the mechanism of action of complexes 1, 3, and 4 differs somewhat from that of Auranofin.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Assessing HCH isomer uptake in Alnus glutinosa: implications for phytoremediation and microbial response},

url = {10.1038/s41598-024-54235-1},

doi = {10.1038/s41598-024-54235-1},

issn = {2045-2322},

year  = {2024},

date = {2024-02-20},

journal = {SCIENTIFIC REPORTS},

volume = {14},

issue = {1},

abstract = {Although the pesticide hexachlorocyclohexane (HCH) and its isomers have long been banned, their presence in the environment is still reported worldwide. In this study, we investigated the bioaccumulation potential of alpha, beta, and delta hexachlorocyclohexane (HCH) isomers in black alder saplings (Alnus glutinosa) to assess their environmental impact. Each isomer, at a concentration of 50 mg/kg, was individually mixed with soil, and triplicate setups, including a control without HCH, were monitored for three months with access to water. Gas chromatography-mass spectrometry revealed the highest concentrations of HCH isomers in roots, decreasing towards branches and leaves, with delta-HCH exhibiting the highest uptake (roots-14.7 mu g/g, trunk-7.2 mu g/g, branches-1.53 mu g/g, leaves-1.88 mu g/g). Interestingly, alpha-HCH was detected in high concentrations in beta-HCH polluted soil. Phytohormone analysis indicated altered cytokinin, jasmonate, abscisate, and gibberellin levels in A. glutinosa in response to HCH contamination. In addition, amplicon 16S rRNA sequencing was used to study the rhizosphere and soil microbial community. While rhizosphere microbial populations were generally similar in all HCH isomer samples, Pseudomonas spp. decreased across all HCH-amended samples, and Tomentella dominated in beta-HCH and control rhizosphere samples but was lowest in delta-HCH samples.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Julia-Kocienski-Like Connective C-C and C=C Bond-Forming Reaction},

author = {David J.-Y. D. Bon and Daniel Chrenko and Ondrej Kovac and Vendula Ferugova and Pavel Lasak and Marketa Fuksova and Frantisek Zalesak and Jiri Pospisi},

issn = {1615-4150},

year  = {2024},

date = {2024-02-19},

journal = {ADVANCED SYNTHESIS & CATALYSIS},

volume = {366},

issue = {3},

pages = {480-487},

abstract = {In this paper, we present a one-pot protocol that enables a straightforward and selective transformation of alkyl benzothiazol-2-yl and phenyltetrazol-2-yl sulfones and acyl chlorides into ketones, E-olefins, Z-olefins, and even pyrroles. The final product of the reaction depends on the proper choice of the reaction workup. Notably, the protocol designed for olefin formation allows a switch between E- and Z-olefin formation by the correct choice of the reaction workup. These developed protocols facilitate the formation of all compounds under mild reaction conditions, as evidenced by the synthesis of (nitro)-fatty acids, and the concept can be extended to other product formations, as demonstrated by the synthesis of pyrroles. +image

},

note = {10.1002/adsc.202301054

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Comparison of plant biostimulating properties of Chlorella sorokiniana biomass produced in batch and semi-continuous systems supplemented with pig manure or acetate},

author = {Wendy A. Stirk and Peter Balint and Jitka Siroka and Ondrej Novak and Tamas Retfalvi and Zoltan Berzsenyi and Jacint Notterpek and Zolten Varga and Gergely Maroti and Johannes van Staden and Miroslav Strnad and Vince Ordog},

url = {10.1016/j.jbiotec.2024.01.002

},

doi = {10.1016/j.jbiotec.2024.01.002},

issn = {0168-1656},

year  = {2024},

date = {2024-02-05},

urldate = {2024-02-05},

journal = {JOURNAL OF BIOTECHNOLOGY},

volume = {381},

pages = {27-35},

abstract = {Microalgae-derived biostimulants provide an eco-friendly biotechnology for improving crop productivity. The strategy of circular economy includes reducing biomass production costs of new and robust microalgae strains grown in nutrient-rich wastewater and mixotrophic culture where media is enriched with organic carbon. In this study, Chlorella sorokiniana was grown in 100 l bioreactors under sub-optimal conditions in a greenhouse. A combination of batch and semi-continuous cultivation was used to investigate the growth, plant hormone and biostimulating effect of biomass grown in diluted pig manure and in nutrient medium supplemented with Na-acetate. C. sorokiniana tolerated the low light (sum of PAR 0.99 +/- 0.18 mol/photons/(m(2)/day)) and temperature (3.7-23.7 degrees C) conditions to maintain a positive growth rate and daily biomass productivity (up to 149 mg/l/day and 69 mg/l/day dry matter production in pig manure and Na-acetate supplemented cultures respectively). The protein and lipid content was significantly higher in the biomass generated in batch culture and dilute pig manure (1.4x higher protein and 2x higher lipid) compared to the Na-acetate enriched culture. Auxins indole-3-acetic acid (IAA) and 2-oxindole-3-acetic acid (oxIAA) and salicylic acid (SA) were present in the biomass with significantly higher auxin content in the biomass generated using pig manure (> 350 pmol/g DW IAA and > 84 pmol/g DW oxIAA) compared to cultures enriched with Na-acetate and batch cultures (< 200 pmol/g DW IAA and < 27 pmol/g DW oxIAA). No abscisic acid and jasmonates were detected. All samples had plant biostimulating activity measured in the mungbean rooting bioassay with the Na-acetate supplemented biomass eliciting higher rooting activity (equivalent to 1-2 mg/l IBA) compared to the pig manure (equivalent to 0.5-1 mg/l IBA) and batch culture (equivalent to water control) generated biomass. Thus C. sorokiniana MACC-728 is a robust new strain for biotechnology, tolerating low light and temperature conditions. The strain can adapt to alternative nutrient (pig manure) and carbon (acetate) sources with the generated biomass having a high auxin concentration and plant biostimulating activity detected with the mungbean rooting bioassay.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{WOS:001105757800001,

title = {Leaf ontogeny modulates epinasty through shifts in hormone dynamics 

 during waterlogging in tomato},

author = {Batist Geldhof and Ondrej Novak and Bram Poel},

doi = {10.1093/jxb/erad432},

issn = {0022-0957},

year  = {2024},

date = {2024-02-01},

journal = {JOURNAL OF EXPERIMENTAL BOTANY},

volume = {75},

number = {3},

pages = {1081-1097},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Mitigation of arsenic poisoning induced oxidative stress and genotoxicity by Ocimum gratissimum L},

author = {Nikhil Mishra and Seema Tripathi and Lutfun Nahar and Satyajit D. Sarker and Anil Kumar},

url = {10.1016/j.toxicon.2024.107603

},

doi = {10.1016/j.toxicon.2024.107603},

issn = {0041-0101},

year  = {2024},

date = {2024-02-01},

journal = {TOXICON},

volume = {238},

abstract = {Arsenic toxicity is a major problem across the world due to geogenic activity and has been supposed to generate free radicals and genotoxicity among the arsenic-poisoned population. There is a need to find suitable free radical quenching compounds for the arsenic-induced free radical-affected population. In the present study, Na3AsO3- induced oxidative stress and genotoxicity were evaluated in Oryctolagus cuniculus L, and quenching competency of Ocimum species was examined by applying enzymatic and non-enzymatic in vitro tests, comet assay, and Random Amplified Polymorphic Deoxyribonucleic acid - Polymerase Chain Reaction (RAPD-PCR) methods. In the present study, oxidative damage due to Na3AsO3 intoxication in O. cuniculus L has been confirmed followed by substantive genotoxicity, and in a further study, it has also been reported that the extract of O. gratissimum L lowers the oxidative stress in experimental animals confirmed by a decrease in Malondialdehyde (MDA) 4.78 +/- 0.05 (nmol/mg protein), and an increase in Glutathione (GSH) 2.87 +/- 0.50 (mu moles/mg proteins), Superoxide Dismutase (SOD) 1.78 +/- 0.03(Units/mg protein), Catalase (CAT) 2.72 +/- 0.02 (mu moles of H2O2 consumed/min/mg proteins) and Glutathione peroxidase (GPX) 7.43 +/- 0.01 (mu g of glutathione utilized/min/mg protein). A positive impact of extract of O. gratissimum L on protection of genotoxicity has been also confirmed by Random Amplified Polymorphic DNA (RAPD) based reduction in polymorphic bands of Deoxyribonucleic acid (DNA) from 6.5 to 3.16 and comet assay-based increase in head DNA % (87.86 +/- 1.58), tail moment (1.07 +/- 0.27) and decrease in tail DNA % (12.13 +/- 1.58) & tail length (8.2 +/- 1.46) at 5% P in lymphocytes. A significant level reduction in free radicals and reduction in DNA polymorphism has proved the competency of test material for the development of suitable antidotes against arsenicosis.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Highlights in gibberellin research: A tale of the dwarf and the slender},

author = {Eilon Shani and Peter Hedden and Tai-ping Sun

},

url = {10.1093/plphys/kiae044},

doi = {10.1093/plphys/kiae044},

issn = {2666-1543},

year  = {2024},

date = {2024-01-30},

urldate = {2024-01-30},

journal = {PLANT PHYSIOLOGY},

volume = {195},

issue = {1},

pages = {111-134},

abstract = {It has been almost a century since biologically active gibberellin (GA) was isolated. Here, we give a historical overview of the early efforts in establishing the GA biosynthesis and catabolism pathway, characterizing the enzymes for GA metabolism, and elucidating their corresponding genes. We then highlight more recent studies that have identified the GA receptors and early GA signaling components (DELLA repressors and F-box activators), determined the molecular mechanism of DELLA-mediated transcription reprograming, and revealed how DELLAs integrate multiple signaling pathways to regulate plant vegetative and reproductive development in response to internal and external cues. Finally, we discuss the GA transporters and their roles in GA-mediated plant development. A historical overview of gibberellin research showcases important advances in our understanding of gibberellin metabolism, perception, signaling, and transport.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Characteristics of turion development in two aquatic carnivorous plants: Hormonal profiles, gas exchange and mineral nutrient content},

author = {Lubomir Adamec and Lenka Plackova and Karel Dolezal},

url = {10.1002/pld3.558

},

doi = {10.1002/pld3.558},

issn = {2475-4455},

year  = {2024},

date = {2024-01-11},

journal = {PLANT DIRECT},

volume = {8},

issue = {1},

pages = {1-11},

abstract = {Turions are vegetative, dormant, and storage overwintering organs formed in perennial aquatic plants in response to unfavorable ecological conditions and originate by extreme condensation of apical shoot segments. The contents of cytokinins, auxins, and abscisic acid were estimated in shoot apices of summer growing, rootless aquatic carnivorous plants, Aldrovanda vesiculosa and Utricularia australis, and in developing turions at three stages and full maturity to reveal hormonal patterns responsible for turion development. The hormones were analyzed in miniature turion samples using ultraperformance liquid chromatography coupled with triple quadrupole mass spectrometry. Photosynthetic measurements in young leaves also confirmed relatively high photosynthetic rates at later turion stages. The content of active cytokinin forms was almost stable in A. vesiculosa during turion development but markedly decreased in U. australis. In both species, auxin content culminated in the middle of turion development and then decreased again. The content of abscisic acid as the main inhibitory hormone was very low in growing plants in both species but rose greatly at first developmental stages and stayed very high in mature turions. The hormonal data indicate a great strength of developing turions within sink-source relationships and confirm the central role of abscisic acid in regulating the turion development.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Involvement of Abscisic Acid in Transition of Pea (Pisum sativum L.) Seeds from Germination to Post-Germination Stages},

author = {Galina Smolikova and Ekaterina Krylova and Ivan Petrik and Polina Vilis and Aleksander Vikhorev and Ksenia Strygina and Miroslav Strnad and Andrej Frolov and Elena Khlestkina and Sergei Medvedev and Margarita A. Vishnyakova},

url = {10.3390/plants13020206

},

doi = {10.3390/plants13020206},

issn = {2223-7747},

year  = {2024},

date = {2024-01-11},

journal = {PLANTS-BASEL},

volume = {13},

issue = {2},

pages = {1-16},

abstract = {The transition from seed to seedling represents a critical developmental step in the life cycle of higher plants, dramatically affecting plant ontogenesis and stress tolerance. The release from dormancy to acquiring germination ability is defined by a balance of phytohormones, with the substantial contribution of abscisic acid (ABA), which inhibits germination. We studied the embryonic axis of Pisum sativum L. before and after radicle protrusion. Our previous work compared RNA sequencing-based transcriptomics in the embryonic axis isolated before and after radicle protrusion. The current study aims to analyze ABA-dependent gene regulation during the transition of the embryonic axis from the germination to post-germination stages. First, we determined the levels of abscisates (ABA, phaseic acid, dihydrophaseic acid, and neo-phaseic acid) using ultra-high-performance liquid chromatography-tandem mass spectrometry. Second, we made a detailed annotation of ABA-associated genes using RNA sequencing-based transcriptome profiling. Finally, we analyzed the DNA methylation patterns in the promoters of the PsABI3, PsABI4, and PsABI5 genes. We showed that changes in the abscisate profile are characterized by the accumulation of ABA catabolites, and the ABA-related gene profile is accompanied by the upregulation of genes controlling seedling development and the downregulation of genes controlling water deprivation. The expression of ABI3, ABI4, and ABI5, which encode crucial transcription factors during late maturation, was downregulated by more than 20-fold, and their promoters exhibited high levels of methylation already at the late germination stage. Thus, although ABA remains important, other regulators seems to be involved in the transition from seed to seedling.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Patients with Neurodegenerative Proteinopathies Exhibit Altered Tryptophan Metabolism in the Serum and Cerebrospinal Fluid},

author = {Michal Kaleta and Eva Henykova and Katerina Mensikova and David Friedecky and Ales Kvasnicka and Katerina Klicova and Dorota Konickova and Miroslav Strnad and Petr Kanovsky and Ondrej Novak},

url = {10.1021/acschemneuro.3c00611

},

doi = {10.1021/acschemneuro.3c00611},

issn = {1948-7193},

year  = {2024},

date = {2024-01-09},

journal = {ACS CHEMICAL NEUROSCIENCE},

volume = {15},

issue = {3},

pages = {582-592},

abstract = {Some pathological conditions affecting the human body can also disrupt metabolic pathways and thus alter the overall metabolic profile. Knowledge of metabolic disturbances in specific diseases could thus enable the differential diagnosis of otherwise similar conditions. This work therefore aimed to comprehensively characterize changes in tryptophan metabolism in selected neurodegenerative diseases. Levels of 18 tryptophan-related neuroactive substances were determined by high throughput and sensitive ultrahigh-performance liquid chromatography-tandem mass spectrometry in time-linked blood serum and cerebrospinal fluid samples from 100 age-matched participants belonging to five cohorts: healthy volunteers (n = 21) and patients with Lewy body disease (Parkinson's disease and dementia with Lewy bodies; n = 31), four-repeat tauopathy (progressive supranuclear palsy and corticobasal syndrome; n = 10), multiple system atrophy (n = 13), and Alzheimer's disease (n = 25). Although these conditions have different pathologies and clinical symptoms, the discovery of new biomarkers is still important. The most statistically significant differences (with p-values of <= 0.05 to <= 0.0001) between the study cohorts were observed for three tryptophan metabolites: l-kynurenine in cerebrospinal fluid and 3-hydroxy-l-kynurenine and 5-hydroxy-l-tryptophan in blood serum. This led to the discovery of distinctive correlation patterns between the profiled cerebrospinal fluid and serum metabolites that could provide a basis for the differential diagnosis of neurodegenerative tauopathies and synucleinopathies. However, further large-scale studies are needed to determine the direct involvement of these metabolites in the studied neuropathologies, their response to medication, and their potential therapeutic relevance.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Utilizing neurodegenerative markers for the diagnostic evaluation of amyotrophic lateral sclerosis},

author = {Katerina Klickova and Jan Mares and Katerina Mensikova and Michaela Kaiserova and David Friedecky and Petr Kanovsky and Miroslav Strnad and Radoslav Matej},

url = {10.1186/s40001-023-01596-4

},

doi = {10.1186/s40001-023-01596-4},

issn = {0949-2321},

year  = {2024},

date = {2024-01-06},

journal = {EUROPEAN JOURNAL OF MEDICAL RESEARCH},

volume = {29},

issue = {1},

pages = {1850-1865},

abstract = {Background Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disorder characterized by progressive deterioration of upper and lower motor neurons. A definitive diagnostic test or biomarker for ALS is currently unavailable, leading to a diagnostic delay following the onset of initial symptoms. Our study focused on cerebrospinal fluid (CSF) concentrations of clusterin, tau protein, phosphorylated tau protein, and beta-amyloid1-42 in ALS patients and a control group.Methods Our study involved 54 ALS patients and 58 control subjects. Among the ALS patients, 14 presented with bulbar-onset ALS, and 40 with limb-onset ALS. We quantified biomarker levels using enzyme-linked immunosorbent assay (ELISA) and compared the results using the Mann-Whitney U-test.Results Significant elevations in neurodegenerative markers, including tau protein (p < 0.0001), phosphorylated tau protein (p < 0.0001), and clusterin (p = 0.038), were observed in ALS patients compared to controls. Elevated levels of tau protein and phosphorylated tau protein were also noted in both bulbar and limb-onset ALS patients. However, no significant difference was observed for beta-amyloid1-42. ROC analysis identified tau protein (AUC = 0.767) and p-tau protein (AUC = 0.719) as statistically significant predictors for ALS.Conclusion Our study demonstrates that neurodegenerative marker levels indicate an ongoing neurodegenerative process in ALS. Nonetheless, the progression of ALS cannot be predicted solely based on these markers. The discovery of a specific biomarker could potentially complement existing diagnostic criteria for ALS.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Rapid profiling of cytokinins using supercritical fluid chromatography coupled with tandem mass spectrometry},

author = {Ivan Petrik and Ales Pencik and Jakub Styskala and Lenka Tranova and Petra Amakorova and Miroslav Strnad and Ondrej Novak},

url = {10.1016/j.aca.2023.342010

},

doi = {10.1016/j.aca.2023.342010},

issn = {0003-2670},

year  = {2024},

date = {2024-01-02},

journal = {ANALYTICA CHIMICA ACTA},

volume = {1285},

abstract = {Background: The determination of plant hormones is still a very challenging analytical discipline, mainly due to their low concentration in complex plant matrices. Therefore, the involvement of very sensitive high-throughput techniques is required. Cytokinins (CKs) are semi-polar basic plant hormones regulating plant growth and development. Modern methods for CK determination are currently based on ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS), which enables the separation of CK isomeric forms occurring endogenously in plants. Here, ultra-high performance supercritical fluid chromatography coupled with tandem mass spectrometry (UHPSFC-MS/MS) was used for the simultaneous determination of 37 CK metabolites. Results: The chromatographic conditions were tested on three different columns with various retention mechanisms. Hybrid silica modified with 2-picolylamine was selected as the stationary phase. Several parameters such as column temperature, back pressure regulation, mobile phase composition and make-up solvent were investigated to achieve efficient separation of CK isomers and reasonable sensitivity. Compared to UHPLC-MS/MS, a 9-min chromatographic analysis using a mobile phase of supercritical CO2 and 5 mM ammonia in methanol represents a three-fold acceleration of total run time. The quantification limit of UHPSFC-MS/MS method was in the range of 0.03-0.19 fmol per injection and the method validation showed high accuracy and precision (below 15 % for most analytes). The method was finally applied to the complex plant matrix of the model plant Arabidopsis thaliana and the obtained profiles of CK metabolites were compared with the results from the conventional UHPLC-MS/MS method. Significance: The presented work offers a novel approach for quantification of endogenous CKs in plants. Compared to the conventional UHPLC-MS/MS, the total run time is shorter and the matrix effect lower for the key CK metabolites. This approach opens the opportunity to utilize UHPSFC-MS/MS instrumentation for targeted plant hormonomics including other plant hormone families.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{WOS:001090650100001,

title = {In situ separation and visualization of isomeric auxin derivatives in 

 \textit{Arabidopsis} by ion mobility mass spectrometry imaging},

author = {Chao Zhang and Kristyna Bieleszova and Asta Zukauskaite and Pavel Hladik and Jiri Gruz and Ondrej Novak and Karel Dolezal},

doi = {10.1007/s00216-023-04996-x},

issn = {1618-2642},

year  = {2024},

date = {2024-01-01},

journal = {ANALYTICAL AND BIOANALYTICAL CHEMISTRY},

volume = {416},

number = {1, SI},

pages = {125-139},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{WOS:001091395300001,

title = {Is the co-option of jasmonate signalling for botanical carnivory a 

 universal trait for all carnivorous plants?},

author = {Andrej Pavlovic and Jana Koller and Ondrej Vrobel and Ivo Chamrad and Rene Lenobel and Petr Tarkowski},

doi = {10.1093/jxb/erad359},

issn = {0022-0957},

year  = {2024},

date = {2024-01-01},

journal = {JOURNAL OF EXPERIMENTAL BOTANY},

volume = {75},

number = {1},

pages = {334-349},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Chloroplast Auxin Efflux Mediated by ABCB28 and ABCB29 Fine-Tunes Salt and Drought Stress Responses in Arabidopsis},

author = {Prashanth Tamizhselvan and Sharmila Madhavan and Christian Constan-Aguilar and Eman Ryad Elrefaay and Jie Liu and Ales Pencik and Ondrej Novak and Albert Cairo and Monika Hrtyan and Markus Geisler and Vanesa Beatriz Tognetti},

url = {10.3390/plants13010007

},

doi = {10.3390/plants13010007},

isbn = {2223-7747},

year  = {2024},

date = {2024-01-01},

journal = {PLANTS-BASEL},

volume = {13},

issue = {1},

pages = {1-26},

abstract = {Photosynthesis is among the first processes negatively affected by environmental cues and its performance directly determines plant cell fitness and ultimately crop yield. Primarily sites of photosynthesis, chloroplasts are unique sites also for the biosynthesis of precursors of the growth regulator auxin and for sensing environmental stress, but their role in intracellular auxin homeostasis, vital for plant growth and survival in changing environments, remains poorly understood. Here, we identified two ATP-binding cassette (ABC) subfamily B transporters, ABCB28 and ABCB29, which export auxin across the chloroplast envelope to the cytosol in a concerted action in vivo. Moreover, we provide evidence for an auxin biosynthesis pathway in Arabidopsis thaliana chloroplasts. The overexpression of ABCB28 and ABCB29 influenced stomatal regulation and resulted in significantly improved water use efficiency and survival rates during salt and drought stresses. Our results suggest that chloroplast auxin production and transport contribute to stomata regulation for conserving water upon salt stress. ABCB28 and ABCB29 integrate photosynthesis and auxin signals and as such hold great potential to improve the adaptation potential of crops to environmental cues.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Application of Long-Chained Auxin Conjugates Influenced Auxin Metabolism and Transcriptome Response in Brassica rapa L. ssp. pekinensis},

author = {Ana Smolko and Jelena Repar and Marija Matkovic and Iva Pavlovic and Ales Pencik and Ondrej Novak and Jutta Ludwig-Mueller and Branka Salopek-Sondi













},

url = {10.3390/ijms25010447},

doi = {10.3390/ijms25010447},

issn = {1661-6596},

year  = {2024},

date = {2024-01-01},

urldate = {2024-01-01},

journal = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES},

volume = {25},

number = {1},

abstract = {Auxin amino acid conjugates are considered to be storage forms of auxins. Previous research has shown that indole-3-acetyl-L-alanine (IAA-Ala), indole-3-propionyl-L-alanine (IPA-Ala) and indole-3-butyryl-L-alanine (IBA-Ala) affect the root growth of Brassica rapa seedlings. To elucidate the potential mechanism of action of the conjugates, we treated B. rapa seedlings with 0.01 mM IAA-, IPA- and IBA-Ala and investigated their effects on the auxin metabolome and transcriptome. IBA-Ala and IPA-Ala caused a significant inhibition of root growth and a decrease in free IAA compared to the control and IAA-Ala treatments. The identification of free auxins IBA and IPA after feeding experiments with IBA-Ala and IPA-Ala, respectively, confirms their hydrolysis in vivo and indicates active auxins responsible for a stronger inhibition of root growth. IBA-Ala caused the induction of most DEGs (807) compared to IPA-Ala (417) and IAA-Ala (371). All treatments caused similar trends in transcription profile changes when compared to control treatments. The majority of auxin-related DEGs were found after IBA-Ala treatment, followed by IPA-Ala and IAA-Ala, which is consistent with the apparent root morphology. In addition to most YUC genes, which showed a tendency to be downregulated, transcripts of auxin-related DEGs that were identified (UGT74E2, GH3.2, SAUR, IAA2, etc.) were more highly expressed after all treatments. Our results are consistent with the hypothesis that the hydrolysis of conjugates and the release of free auxins are responsible for the effects of conjugate treatments. In conclusion, free auxins released by the hydrolysis of all auxin conjugates applied affect gene regulation, auxin homeostasis and ultimately root growth inhibition.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Major Bioactive Prenylated Flavonoids from Humulus lupulus L., Their Applications in Human Diseases and Structure-Activity Relationships (SAR) - A Review},

author = {Ferah Comert Onder and Sevil Kalin and Nebahat Sahin and Gulce Davutlar and Khaled A. N. Abusharkh and Ozlem Maraba and Rabia Selina Hal and Mehmet Ay and Lutfun Nahar and Satyajit D. Sarker},

url = {10.34172/PS.2023.18},

doi = {10.34172/PS.2023.18},

issn = {1735-403X},

year  = {2024},

date = {2024-01-01},

journal = {PHARMACEUTICAL SCIENCES},

volume = {30},

issue = {1},

pages = {1-20},

abstract = {In recent years, the incidence of cancers, inflammatory diseases, Alzheimer's disease, glucose metabolism disorder and diabetes has increased alarmingly which demands more research into the discovery of new drug candidates to treat these human diseases. Main phytochemicals from Humulus lupulus L. (hops) have been demonstrated to have positive impacts on human health, and prenylated flavonoids are one of the major groups of bioactive phytochemicals found in this plant. Thus, this review summarizes the role of major prenylated components in hops in human diseases including cancer, inflammation and viral infections. In silico studies of prenylated bioactive compounds against various drug targets such as histone deactylases (HDACs), sirtuins (SIRTs), and acetylcholinesterase (AChE), and the molecular molecular interactions between protein and ligand have also been included. Furthermore, the structure-activity relationships (SAR) studies on these compounds are highlighted. This review concludes that the prenylated phytochemicals from H. lupulus L., including xanthohumol (XN), isoxanthohumol (IXN), 8-prenylnaringenin (8-PN) and 6-prenylnaringenin (6-PN), have promising roles in human health and may contribute to new drug discovery and development.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Stability of Piceatannol in Dulbecco's Modified Eagle's Medium by In Situ UPLC-MS/MS Analysis},

author = {Meng S. Cheong and Lutfun Nahar and Satyajit D. Sarker and Hui Cao and Wai S. Cheang and Jianbo Xiao},

url = {10.1177/1934578X221111036

},

doi = {10.1177/1934578X221111036},

issn = {1934-578X},

year  = {2024},

date = {2024-01-01},

journal = {NATURAL PRODUCT COMMUNICATIONS},

volume = {19},

issue = {1},

pages = {1-19},

abstract = {Piceatannol is a stilbenoid, which has shown bioactivities in various cell culture models. However, its stability in cell culture medium is not clear. Here, UPLC-MS/MS was applied in situ to analyze the degradation products of piceatannol in Dulbecco's Modified Eagle's Medium (DMEM) and cell culture to investigate the compound's stability in DMEM. During the incubation with cell culture medium (at 4 and 37 degrees C), several piceatannol derivatives, such as an oxidation product (m/z 243.06), a reduction product (m/z 247.09), dimers (m/z 485.12 and 487.14) and trimers (m/z 727.18) were detected, which demonstrated the instability of piceatannol in cell culture conditions. To confirm if the new products during the incubation were generated due to the instability of piceatannol, ascorbic acid was added. The presence of ascorbic acid could significantly slow the degradation rate of piceatannol and the generation of piceatannol derivatives, which proved that the new products were generated by the degradation of piceatannol and indicated that the instability of piceatannol might be related to its antioxidant activity.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{WOS:001104123300001,

title = {Application of gas chromatography in the analysis of phytocannabinoids:  An update (2020-2023)},

author = {Lutfun Nahar and Georgiana-Luminita Gavril and Satyajit D. Sarker},

url = {10.1002/pca.3303},

doi = {10.1002/pca.3303},

issn = {0958-0344},

year  = {2023},

date = {2023-12-01},

urldate = {2023-12-01},

journal = {PHYTOCHEMICAL ANALYSIS},

volume = {34},

number = {8},

pages = {903-924},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{WOS:001070054000001,

title = {Fluorescence-activated multi-organelle mapping of subcellular plant  hormone distribution},

author = {Vladimir Skalicky and Ioanna Antoniadi and Ales Pencik and Ivo Chamrad and Rene Lenobel and Martin F. Kubes and Marek Zatloukal and Asta Zukauskaite and Miroslav Strnad and Karin Ljung and Ondrej Novak},

url = {10.1111/tpj.16456},

doi = {10.1111/tpj.16456},

issn = {0960-7412},

year  = {2023},

date = {2023-12-01},

urldate = {2023-12-01},

journal = {PLANT JOURNAL},

volume = {116},

number = {6},

pages = {1825-1841},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{WOS:001105924400001,

title = {Are\textit{ Arabidopsis}\textit{ thaliana} plants able to recover from  exposure to gamma radiation? A molecular},

author = {Nele Horemans and Jackline Kariuki and Eline Saenen and Mohamed Mysara and Gerrit T. S. Beemster and Katrien Sprangers and Iva Pavlovic and Ondrej Novak and May Van Hees and Robin Nauts and Gustavo Turqueto Duarte and Ann Cuypers},

url = {10.1016/j.jenvrad.2023.107304},

doi = {10.1016/j.jenvrad.2023.107304},

issn = {0265-931X},

year  = {2023},

date = {2023-12-01},

urldate = {2023-12-01},

journal = {JOURNAL OF ENVIRONMENTAL RADIOACTIVITY},

volume = {270},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{WOS:001085559700001,

title = {Flexure wood formation via growth reprogramming in hybrid aspen involves  jasmonates and polyamines and transcriptional changes resembling tension  wood development},

author = {Janos Urbancsok and Evgeniy N. Donev and Pramod Sivan and Elena Zalen and Felix R. Barbut and Marta Derba-Maceluch and Jan Simura and Zakiya Yassin and Madhavi L. Gandla and Michal Karady and Karin Ljung and Sandra Winestrand and Leif J. Jonsson and Gerhard Scheepers and Nicolas Delhomme and Nathaniel R. Street and Ewa J. Mellerowicz},

url = {10.1111/nph.19307},

doi = {10.1111/nph.19307},

issn = {0028-646X},

year  = {2023},

date = {2023-12-01},

urldate = {2023-12-01},

journal = {NEW PHYTOLOGIST},

volume = {240},

number = {6},

pages = {2312-2334},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{WOS:000994554300001,

title = {New PEO-IAA-Inspired Anti-Auxins: Synthesis, Biological Activity, and  Possible Application in Hemp (\textit{Cannabis Sativa} L.)  Micropropagation},

author = {Asta Zukauskaite and Inigo Saiz-Fernandez and Kristyna Bieleszova and Monika Iskauskiene and Chao Zhang and Iva Smykalova and Karolina Dzedulionyte and Martin F. Kubes and Michaela Sedlarova and Barbora Parizkova and Iva Pavlovic and Thomas Vain and Ivan Petrik and Vida Malinauskiene and Algirdas Sackus and Miroslav Strnad and Stephanie Robert and Richard Napier and Ondrej Novak and Karel Dolezal},

url = {10.1007/s00344-023-11031-x},

doi = {10.1007/s00344-023-11031-x},

issn = {0721-7595},

year  = {2023},

date = {2023-12-01},

urldate = {2023-12-01},

journal = {JOURNAL OF PLANT GROWTH REGULATION},

volume = {42},

number = {12},

pages = {7547-7563},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{WOS:001110294300001,

title = {Acute and sub-acute oral toxicity assessment of the methanol root  extract of\textit{ Olax}\textit{ subscorpioidea} Oliv. (Olacaceae) in mice  and rats},

author = {Yemi A. Adekunle and Babatunde B. Samuel and Joshua U. Ezeuduji and Oluwasegun A. Adedokun and Wande M. Oluyemi and Lutfun Nahar and Amos A. Fatokun and Satyajit D. Sarker},

url = {10.1016/j.sajb.2023.10.051},

doi = {10.1016/j.sajb.2023.10.051},

issn = {0254-6299},

year  = {2023},

date = {2023-12-01},

urldate = {2023-12-01},

journal = {SOUTH AFRICAN JOURNAL OF BOTANY},

volume = {163},

pages = {157-164},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{WOS:001069048300001,

title = {Ectomycorrhizal symbiosis prepares its host locally and systemically for  abiotic cue signaling},

author = {Maira de Freitas Pereira and David Cohen and Lucas Auer and Nathalie Aubry and Marie-Beatrice Bogeat-Triboulot and Cyril Bure and Nancy L. Engle and Yves Jolivet and Annegret Kohler and Ondrej Novak and Iva Pavlovic and Pierrick Priault and Timothy J. Tschaplinski and Irene Hummel and Marie-Noelle Vaultier and Claire Veneault-Fourrey},

url = {10.1111/tpj.16465},

doi = {10.1111/tpj.16465},

issn = {0960-7412},

year  = {2023},

date = {2023-12-01},

urldate = {2023-12-01},

journal = {PLANT JOURNAL},

volume = {116},

number = {6},

pages = {1784-1803},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{WOS:001078968900001,

title = {Synthesis and neuroprotective activity of 3-aryl-3-azetidinyl acetic  acid methyl ester derivatives},

author = {Urte Sachleviciute and Gabriel Gonzalez and Marie Kvasnicova and Sarka Stepankova and Neringa Kleiziene and Aurimas Bieliauskas and Marek Zatloukal and Miroslav Strnad and Frank A. Slok and Miroslav Kvasnica and Algirdas Sackus and Asta Zukauskaite},

url = {10.1002/ardp.202300378},

doi = {10.1002/ardp.202300378},

issn = {0365-6233},

year  = {2023},

date = {2023-12-01},

urldate = {2023-12-01},

journal = {ARCHIV DER PHARMAZIE},

volume = {356},

number = {12},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{WOS:001077363900001,

title = {Synthetic and analytical routes to the L-amino acid conjugates of  cis-OPDA and their identification and quantification in plants},

author = {Vaclav Mik and Tomas Pospisil and Federica Brunoni and Jiri Gruz and Vladimfra Nozkova and Claus Wasternack and Otto Miersch and Miroslav Strnad and Kristyna Flokova and Ondrej Novak and Jitka Siroka},

url = {10.1016/j.phytochem.2023.113855},

doi = {10.1016/j.phytochem.2023.113855},

issn = {0031-9422},

year  = {2023},

date = {2023-11-01},

urldate = {2023-11-01},

journal = {PHYTOCHEMISTRY},

volume = {215},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{WOS:001080637200001,

title = {Cytotoxic triterpenoid saponins from the root of Olax subscorpioidea  Oliv. (Olacaceae)},

author = {Yemi A. Adekunle and Babatunde B. Samuel and Lutfun Nahar and Amos A. Fatokun and Satyajit D. Sarker},

url = {10.1016/j.phytochem.2023.113853},

doi = {10.1016/j.phytochem.2023.113853},

issn = {0031-9422},

year  = {2023},

date = {2023-11-01},

urldate = {2023-11-01},

journal = {PHYTOCHEMISTRY},

volume = {215},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{WOS:001041610200001,

title = {Sodium and chloride accumulation and repartition differed between the  cultivated tomato\textit{ (Solanum}\textit{ lycopersicum)} and its wild  halophyte relative\textit{ Solanum}\textit{ chilense} under salt stress},

author = {Servane Bigot and Marketa Fuksova and Juan-Pablo Martinez and Stanley Lutts and Muriel Quinet},

url = {10.1016/j.scienta.2023.112324},

doi = {10.1016/j.scienta.2023.112324},

issn = {0304-4238},

year  = {2023},

date = {2023-11-01},

urldate = {2023-11-01},

journal = {SCIENTIA HORTICULTURAE},

volume = {321},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{WOS:000972310800001,

title = {A Differential Phytohormone Profile in the Aerial Part and Roots as a  Response to Water Stress Underlying Morphophysiological and Biochemical  Changes in Two Inbred Sunflower Lines at Early Growth Stage},

author = {Aldana Boero and Federico Ramirez and Jana Oklestkova and Ana Vigliocco and Miroslav Strnad and Sergio Alemano and Andrea Andrade},

url = {10.1007/s00344-023-10999-w},

doi = {10.1007/s00344-023-10999-w},

issn = {0721-7595},

year  = {2023},

date = {2023-11-01},

urldate = {2023-11-01},

journal = {JOURNAL OF PLANT GROWTH REGULATION},

volume = {42},

number = {11},

pages = {7083-7095},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{WOS:001020376800001,

title = {Plasmodesmata mediate cell-to-cell transport of brassinosteroid hormones},

author = {Yaowei Wang and Jessica Perez-Sancho and Matthieu Pierre Platre and Brenda Callebaut and Marija Smokvarska and Karoll Ferrer and Yongming Luo and Trevor M. M. Nolan and Takeo Sato and Wolfgang Busch and Philip N. N. Benfey and Miroslav Kvasnica and Johan M. M. Winne and Emmanuelle M. M. Bayer and Nemanja Vukasinovic and Eugenia Russinova},

url = {10.1038/s41589-023-01346-x},

doi = {10.1038/s41589-023-01346-x},

issn = {1552-4450},

year  = {2023},

date = {2023-11-01},

urldate = {2023-11-01},

journal = {NATURE CHEMICAL BIOLOGY},

volume = {19},

number = {11},

pages = {1331+},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{WOS:001048888700001,

title = {Frankincense of\textit{ Boswellia}\textit{ sacra:} Traditional and modern  applied uses, pharmacological activities, and clinical trials},

author = {Shaden A. M. Khalifa and Safaa M. Kotb and Shaden H. El-Seedi and Lutfun Nahar and Satyajit D. Sarker and Zhiming Guo and Xiaobo Zou and Syed G. Musharraf and Amir Reza Jassbi and Ming Du and Hesham R. El-Seedi},

url = {10.1016/j.indcrop.2023.117106},

doi = {10.1016/j.indcrop.2023.117106},

issn = {0926-6690},

year  = {2023},

date = {2023-11-01},

urldate = {2023-11-01},

journal = {INDUSTRIAL CROPS AND PRODUCTS},

volume = {203},

keywords = {},

pubstate = {published},

tppubtype = {article}

}