Interdisciplinární přístupy pro vývoj a aplikace nových materiálů v medicínské praxi – Nové omické technologie

The project focuses on research in the field of state-of-the-art applications of omics technologies and the connection of specific methodological approaches (genomics, transcriptomics, proteomics, metabolomics and hormonomics) with a focus on the development of diagnostic applications in the field of medical sciences and medical clinical practice. The project aims to synergistically support the development of a regionally oriented and, in accordance with the integrated territorial strategy, transfer state-of-the-art omics technologies to healthcare in Olomouc region.

NEW OMICS TECHNOLOGIES

Subtask 1. Sequencing and RNA-seq technologies, whole-genome sequencing

Implementation and development of sequencing technologies for medical and biotechnological purposes (especially transcriptomics, RNA-seq technologies and whole-genome sequencing for patient sample analyses) and robust data and bioinformatics tools suitable for high-throughput analysis (e.g. whole-genome data and evaluation of SNP genetic variants within the framework of proposed genetic association studies).

1. Objective 1.1. Genetic and epigenetic aberrations of B-cell malignancies
2. Objective 1.2. Identification of genetic and epigenetic factors involved in the formation of Gd-IgA1 in response to inflammatory signals
   Eva Kriegová, Milan Raška
3. Objective 1.3. Characterization of genomic changes in monogenic, oligogenic and polygenic neurodegenerative genetic diseases
   Radek Vodička, Ondřej Plíhal
4. Objective 1.4. Genomic analysis to detect pathogenic changes in DNA, especially in patients with cardiopathies and ciliopathies, in whom a clear genetic cause of the disease was not detected using standard NGS panel examinations.
5. Objective 1.5. Segregation analysis in selected families of probands, where pathogenic/probably pathogenic variants and variants of unclear significance, reclassified variants, or hypomorphic/modifying polymorphisms in one gene or in multiple related genes were found.
   Tomáš Gucký, Jana Petřková

Subtask 2. Development of standard proteomic procedures

Implementation and development of standard proteomic procedures for the analysis of neurodegenerative disease samples in the clinical laboratory

1. Objective 2.1. Comprehensive untargeted proteomic analysis of neurodegenerative disease samples
2. Objective 2.2. Development of targeted proteomic procedures for the quantification of a selected group of protein markers for neurodegenerative diseases
3. Objective 2.3. Application of established proteomic procedures to other types of diseases and samples.

Activity 1: Implementation of cooperation within the framework of a project partnership

• Establishment of an online communication environment
• Establishment of a web environment
• Regular team meetings
• Exchange of workers

Activity 2: Establishing and developing cooperation with entities in the application sphere

• Organization of thematic workshops
• Visits to regional companies
• Implementation of intellectual property protection and technology transfer
• Longer-term mutual cooperation between NGOs and the corporate sector

Activity 3: Establishing and developing new international cooperation between research teams and the application sphere

• Increasing the number of participations in international cooperation programs
• Developing strategic cooperation and partnerships
• Increasing publication activity with the involvement of foreign workplaces
• Strengthening lecturing and education
• Strengthening foreign team activities and internships
• Involving team members in international organizations
• Organizing international meetings

Activity 4: Promotion of Research Results and Support for the Adoption of New Technologies

• Establishment and organizational implementation of an internal project system to enhance cooperation between research organizations and the application sector
• Creation of a shared online tool and website with project partners for information exchange
• Preparation and promotion of a plan for public lectures, open days, and educational events
• Implementation of planned educational and training activities
• Organization of workshops and twinning events
• Organization and hosting of two international meetings
• Strengthening cooperation with application-sector entities in the Czech Republic
• Development of strategic international collaborations and partnerships
• Involvement of team members in international organizations
• Increased participation in international professional events
• Expansion of international stays of team members

Open Science Principles

• Easier sharing of knowledge should, among other things, speed up and streamline research, improve its reproducibility, support broad collaboration, and accelerate the implementation of innovations into practice.
  The basic elements of open science are open access to scientific articles and other publications (Open Access) and open research data (Open Data).

Documents

Open Science at Palacký University Olomouc

Open scientific procedures within the framework of OP JAK

PU Strategic Plan 2021+

Read & Publish Agreements – the possibility of publishing in open access without APC or with a discount

Institutional Digital Repository