Publikace

@article{nokey,

title = {Gibberellin-induced parthenocarpy in fruits of a prickly pear mutant},

author = {Rameshkumar Ramakrishnan and Udi Zurgil and Shamili Kanna and Danuše Tarkowská and Ondřej Novák and Miroslav Strnad and Noemi Tel-Zur and Yaron Sitrit },

doi = {10.1007/s00299-025-03568-w},

year  = {2025},

date = {2025-08-06},

journal = {Plant Cell Reports},

volume = {44},

number = {194},

pages = {1-15},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Water Shortage-Caused Changes in Endogenous Brassinosteroid Contents in Maize Leaves Depend on the Brassinosteroid Type and Occur Already at the Beginning of the Stress Period},

author = {Hana Marková and Danuše Tarkowska and Petr Čečetka and Marie Kočová and Olga Rothová and Dana Holá



},

doi = {10.1007/s00344-025-11821-5},

year  = {2025},

date = {2025-07-26},

urldate = {2025-07-26},

journal = {Journal of Plant Growth Regulation },

pages = {1-8},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Gibberellin-deactivating GA2OX enzymes act as a hub for auxin–gibberellin cross talk in Arabidopsis thaliana root growth regulation},

author = {Monika Kubalová and Jayne Griffiths and Karel Müller and Lev Levenets and Edita Tylová and Danuše Tarkowská and Alexander M. Jones and Matyáš Fendrych

},

doi = {10.1073/pnas.2425574122},

year  = {2025},

date = {2025-07-22},

urldate = {2025-07-22},

journal = {PNAS},

volume = {122},

issue = {30},

pages = {1-10},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Cytokinin depends on GA biosynthesis and signaling to regulate different aspects of vegetative phase change in Arabidopsis},

author = {Sören Werner and Danuše Tarkowskà and Thomas Schmülling},

doi = {10.1038/s41467-025-61507-5},

year  = {2025},

date = {2025-07-08},

journal = {Nature Communications},

volume = {16},

number = {6292 },

pages = {1-12},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Metabolomics: Potential non-protein biomarker candidates of Parkinson's disease},

author = {Michal Kaleta and Gabriel Gonzalez and Eva Hényková and Kateřina Menšíková and Dorota Koníčková and Petr Kaňovský

},

doi = {10.1016/j.neubiorev.2025.106310},

year  = {2025},

date = {2025-06-30},

urldate = {2025-06-30},

journal = {Neuroscience & Biobehavioral Reviews},

volume = {176},

number = {106310 },

keywords = {},

pubstate = {forthcoming},

tppubtype = {article}

}

@article{nokey,

title = {The carnivorous rainbow plant Byblis filifolia Planch. secretes digestive enzymes in response to prey capture independently of jasmonates},

author = {Andrej Pavlovič and Tereza Jílková and Ivo Chamrád and René Lenobel and Ondřej Vrobel and Petr Tarkowski},

doi = {10.1111/plb.70029},

isbn = {1435-8603},

year  = {2025},

date = {2025-06-19},

urldate = {2025-06-19},

journal = {Plant Biology},

volume = {27},

pages = {549-557},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Regio- and Stereoselective Synthesis of Nitro-fatty Acids as NRF2 Pathway Activators Working under Ambient or Hypoxic Conditions},

author = {Daniel Chrenko and Jana Pereckova and Martina Zatloukalová and Jan Vacek and Jiri Pospisil and Tomas Perecko},

doi = {10.1021/acs.jmedchem.5c00982},

year  = {2025},

date = {2025-05-26},

journal = {Journal of Medicinal Chemistry},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Induced variation in BRASSINOSTEROID INSENSITIVE 1 (BRI1) confers a compact wheat architecture},

author = {Manpartik S. Gill and Andrew L. Phillips and Danuše Tarkowská and John Addy and Patrycja Sokolowska and M. John Foulkes and Stephen Pearce and Stephen G. Thomas and Peter Hedden},

doi = {10.1186/s12870-025-06762-w},

isbn = {1471-2229},

year  = {2025},

date = {2025-05-26},

journal = {BMC PLANT BIOLOGY},

number = {700},

issue = {25},

pages = {1-15},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Brassinolide and BZR1 are up-regulated in a parthenocarpic mutant of prickly pear},

author = {Rameshkumar Ramakrishnan and Udi Zurgil and Danuše Tarkowská and Ondřej Novák and Miroslav Strnad and Noemi Tel-Zur and Yaron Sitrit},

doi = {10.1007/s00299-025-03514-w},

year  = {2025},

date = {2025-05-23},

urldate = {2025-05-23},

journal = {Plant Cell Reports},

volume = {44},

number = {131},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {RT-QuIC: a highly promising diagnostic method for neurodegenerative diseases—advantages and limitations},

author = {Dorota Koníčková and Dominik Hraboš and Kateřina Menšíková and Lucie Tučková and Michal Kaleta and Miroslav Strnad and Carlo Colisimo and Petr Kaňovský},

doi = {/10.3389/fneur.2025.1578252},

year  = {2025},

date = {2025-04-27},

journal = {FRONTIER IN NEUROLOGY },

volume = {16},

pages = {1-11},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Targeted analysis of seven selected tryptophan-melatonin metabolites: Simultaneous quantification of plasma analytes using fast and sensitive UHPLC–MS/MS},

author = {Michal Kaleta and Bhagya S. Kolitha and Ondřej Novák and Farshid Mashayekhy Rad and Jonas Bergquist and S.J. Kumari A. Ubhayasekera },

doi = {10.1016/j.jchromb.2025.124520},

year  = {2025},

date = {2025-04-15},

journal = {Journal of Chromatography B},

volume = {1256},

issue = {124520},

pages = {1-9},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {GIBBERELLIN 3-OXIDASE genes regulate height and grain size in bread wheat },

author = {Andrew L Phillips and Alison K Huttly and Rocío Alarcón-Reverte and Suzanne J Clark and Pavel Jaworek and Danuše Tarkowská and Patrycja Sokolowska and David Steele and Andrew Riche and Malcolm J Hawkesford and Stephen G Thomas and Peter Hedden and Stephen Pearce},

doi = {10.1093/jxb/eraf151},

isbn = {1460-2431 },

year  = {2025},

date = {2025-04-10},

journal = {JOURNAL OF EXPERIMENTAL BOTANY},

pages = {1-14},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Blue or far-red light supplementation induced pre-hardening in the leaves of the Rht12 wheat dwarfing line: hormonal changes and freezing tolerance},

author = {Zsolt Gulyás and Mohamed Ahres and Tamás Pálmai and Kitti Kulman and Zahra Tahmasebi and Kalpita Singh and Kristóf Jobbágy and Danuše Tarkowská and Petre Dobrev and Radomíra Vanková and Péter Borbély and Andreas Börner and Gábor Galiba},

doi = {10.1111/ppl.70112},

year  = {2025},

date = {2025-03-03},

urldate = {2025-03-03},

journal = {PHYSIOLOGIA PLANTARUM},

volume = {177},

issue = {2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {A DOF transcriptional repressor-gibberellin feedback loop plays a crucial role in modulating light-independent seed germination},

author = {Andrea Lepri and Hira Kazmi and Gaia Bertolotti and Chiara Longo and Sara Occhigrossi and Luca Quattrocchi and Mirko De Vivo and Daria Scintu and Noemi Svolacchia and Danuse Tarkowska and Veronika Tureckova and Miroslav Strnad and Marta Del Bianco and Riccardo Di Mambro and Paolo Costantino and Sabrina Sabatini and Raffaele Dello Ioio and Paola Vittorioso },

doi = {10.1016/j.xplc.2025.101262},

year  = {2025},

date = {2025-01-27},

urldate = {2025-01-27},

journal = {Plant Communications },

volume = {6},

number = {101262},

pages = {1-17},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Sulfonation of IAA in Urtica eliminates its DR5 auxin activity},

author = {Klara Supikova and Asta Žukauskaitė and Andrea Kosinova and Aleš Pěnčík and Nuria De Diego and Lukáš Spíchal and Martin Fellner and Katerina Skorepova and Jiri Gruz},

url = {10.1007/s00299-024-03399-1},

doi = {10.1007/s00299-024-03399-1},

year  = {2025},

date = {2025-01-01},

journal = {Plant Cell Reports},

volume = {44},

number = {8},

abstract = {A novel auxin derivative, N-sulfoindole-3-acetic acid (IAA-N-SO3H, SIAA), was discovered in stinging nettle (Urtica dioica) among 116 sulfonated metabolites putatively identified by a semi-targeted UHPLC–QqTOF-MS analysis of 23 plant/algae/fungi species. These sulfometabolites were detected based on the presence of a neutral loss of sulfur trioxide, as indicated by the m/z difference of 79.9568 Da in the MS2 spectra. The structure of newly discovered SIAA was confirmed by synthesizing its standard and comparing retention time, m/z and MS2 spectrum with those of SIAA found in Urtica. To study its natural occurrence, 73 species in total were further analyzed by UHPLC–QqTOF-MS or targeted UHPLC–MS/MS method with a limit of detection of 244 fmol/g dry weight. However, SIAA was only detected in Urtica at a concentration of 13.906 ± 9.603 nmol/g dry weight. Its concentration was > 30 times higher than that of indole-3-acetic acid (IAA), and the SIAA/IAA ratio was further increased under different light conditions, especially in continuous blue light. In addition to SIAA, structurally similar metabolites, N-sulfoindole-3-lactic acid, 4-(sulfooxy)phenyllactic acid and 4-(sulfooxy)phenylacetic acid, were detected in Urtica for the first time. SIAA was biosynthesized from inorganic sulfate in seedlings, as confirmed by the incorporation of exogenous 34S-ammonium sulfate (1 mM and 10 mM). SIAA exhibited no auxin activity, as demonstrated by both the Arabidopsis DR5::GUS assay and the Arabidopsis phenotype analysis. Sulfonation of IAA may therefore be a mechanism for IAA deactivation and/or storage in Urtica, similar to sulfonation of the jasmonates in Arabidopsis.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Amide conjugates of the jasmonate precursor cis-(+)-12-oxo-phytodienoic acid regulate its homeostasis during plant stress responses},

author = {Jitka Siroka and Anita Ament and Vaclav Mik and Tomas Pospisil and Michaela Kralova and Chao Zhang and Marketa Pernisova and Michal Karady and Vladimira Nozkova and Yuho Nishizato and Takuya Kaji and Rina Saito and Mohamed Htitich and Kristyna Flokova and Claus Wasternack and Miroslav Strnad and Minoru Ueda and Ondrej Novak and Federica Brunoni},

url = {10.1093/plphys/kiae636

},

doi = {10.1093/plphys/kiae636},

issn = {0032-0889},

year  = {2024},

date = {2024-12-16},

journal = {PLANT PHYSIOLOGY},

volume = {197},

issue = {1},

abstract = {Jasmonates are a family of oxylipin phytohormones regulating plant development and growth and mediating defense versus growth responses. The upstream JA biosynthetic precursor cis-(+)-12-oxo-phytodienoic acid (cis-OPDA) acts independently of CORONATIVE INSENSITIVE 1-mediated JA signaling in several stress-induced and developmental processes. However, its perception and metabolism are only partially understood. An isoleucine analog of the biologically active JA-Ile, OPDA-Ile, was detected years ago in wounded leaves of flowering plants, opening up the possibility that conjugation of cis-OPDA to amino acids might be a relevant mechanism for cis-OPDA regulation. Here, we extended the analysis of amino acid conjugates of cis-OPDA and identified naturally occurring OPDA-Val, OPDA-Phe, OPDA-Ala, OPDA-Glu, and OPDA-Asp accumulating in response to biotic and abiotic stress in Arabidopsis (Arabidopsis thaliana). The OPDA amino acid conjugates displayed cis-OPDA-related plant responses in a JA-Ile-dependent manner. We also showed that the synthesis and hydrolysis of cis-OPDA amino acid conjugates are mediated by members of the amidosynthetase GRETCHEN HAGEN 3 and the amidohydrolase INDOLE-3-ACETYL-LEUCINE RESISTANT 1/ILR1-like families. Thus, OPDA amino acid conjugates function in the catabolism or temporary storage of cis-OPDA in stress responses instead of acting as chemical signals per se. Amide conjugate synthesis and hydrolysis control the homeostasis of a jasmonate precursor during stress responses in Arabidopsis.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Phenolic Profile of Seedless Ziziphus mauritiana Fruits and Leaves Extracts with In Vivo Antioxidant and Anti-Inflammatory Activities: Influence on Pro-Inflammatory Mediators},

author = {Arifa Khanam and Abdullah Ijaz Hussain and Esraa Haji Mohammed and Lutfun Nahar and Hassaan A. Rathore},

url = {10.1002/cbdv.202401728

},

doi = {10.1002/cbdv.202401728},

issn = {1612-1872},

year  = {2024},

date = {2024-12-12},

journal = {CHEMISTRY & BIODIVERSITY},

abstract = {The present study aimed to assess the antioxidant and anti-inflammatory activities of polyphenol-rich extracts of seedless variety of Ziziphus mauritiana (SZM). Reverse Phase High Performance Liquid Chromatography (RP-HPLC) analysis of SZM leaves and fruit extracts in ethanol revealed the presence of sixteen phenolics including chlorogenic acid, p-coumeric acid, gallic acid, kaempferol, rutin and quercetin. Leaf extract showed higher total phenolic and total flavonoid contents (177.6 mg/100 g and 46.2 mg/100 g) than in fruit extract (137.8 mg/100 g and 14.1 mg/100 g). The leaf extract exhibited higher DPPH radical-scavenging activity (63.5 %) than the fruit extract (58.2 %). The anti-inflammatory activity was evaluated on carrageenan-induced rat model and suppression of inflammatory biomarkers (Interleukin-6, Tumor necrosis factor-alpha and CRP) were studied. The fruit extract exhibited remarkable inhibition (98.1 %) at the dose level of 500 mg/kg body weight (BW), comparable to the standard drug indomethacin (98.4 %). Both extracts suppressed the inflammatory biomarkers and more pronounced results showed by the fruit extract including CRP, IL-6, and TNF-alpha. The leaf extract demonstrated the higher antioxidant potential as evident from the superoxide dismutase, catalase, malondialdehyde, glutathione peroxidase and glutathione levels. These findings suggest that SZM leaf and fruit extracts possess potential antioxidant and remarkable anti-inflammatory properties and can play a significant role in mitigating oxidative stress.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Raw biowaste conversion to high-value compounds for food, cosmetic and pharmaceutical industries},

author = {Veronika Krbeckova and Daniela Placha},

url = {10.1016/j.envres.2024.120134

},

doi = {10.1016/j.envres.2024.120134},

issn = {0013-9351},

year  = {2024},

date = {2024-12-05},

journal = {ENVIRONMENTAL RESEARCH},

volume = {263},

abstract = {Biowaste valorisation into high-value compounds is one of the main challenges of green chemistry, as chemicals produced from biological sources are identified as key substances in the development of a low-carbon and circular bioeconomy in connection with the transition from fossil to renewable feedstocks. The review summarizes the production of high-value products such as glucose-based chemicals, phenolic compounds and volatile-fatty acids prepared from biomass waste. Biowaste pretreatment methods such as milling, filtration and extraction followed by current non-catalytic methods such as microwave or ultrasound extraction and catalytic methods for the production value-added compounds in the presence of various catalyst types in conventional, nano or enzyme form are listed with a focus on value-added chemicals applied in the food, cosmetic and pharmaceutical industries. The economic feasibility, technical aspects and concept of the biorefinery are briefly mentioned, emphasizing the necessity of life cycle assessment for each bioproduct and technological process. Finally, it provides a future perspective and makes recommendations for potential research strategies, recognizing the importance of utilizing biomass waste for the production of useful compounds as an attractive and environmentally friendly approach whose development should be encouraged. The utilization of biowaste for high-value chemicals production shows high potential, however, there are still many challenges to be resolved throughout the entire production chain, reflecting technological, economic, ecological, sociological and long-term issues.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {A decoy receptor derived from alternative splicing fine-tunes cytokinin signaling in Arabidopsis},

author = {Michaela Kralova and Ivona Kubalova and Jakub Hajny and Karolina Kubiasova and Karolina Vagaska and Zengxiang Ge and Michelle Gallei and Hana Semeradova and Anna Kucharova and Martin Hoenig and Aline Monzer and Martin Kovacik and Jiri Friml and Ondrej Novak and Eva Benkova and Yoshihisa Ikeda and David Zalabak},

url = {10.1016/j.molp.2024.11.001

},

doi = {10.1016/j.molp.2024.11.001},

issn = {1674-2052},

year  = {2024},

date = {2024-12-02},

journal = {MOLECULAR PLANT},

volume = {17},

issue = {12},

pages = {1850-1865},

abstract = {Hormone perception and signaling pathways have a fundamental regulatory function in the physiological processes of plants. Cytokinins, a class of plant hormones, regulate cell division and meristem maintenance. The cytokinin signaling pathway is well established in the model plant Arabidopsis thaliana. Several negative feedback mechanisms, tightly controlling cytokinin signaling output, have been described previously. In this study, we identified a new feedback mechanism executed through alternative splicing of the cytokinin receptor AHK4/CRE1. A novel splicing variant named CRE1int'results from seventh intron retention, introducing a premature termination codon in the transcript. We showed that CRE1int' is translated in planta into a truncated receptor lacking the C-terminal receiver domain essential for signal transduction. CRE1int7 can bind cytokinin but cannot activate the downstream cascade. We present a novel negative feedback mechanism of the cytokinin signaling pathway, facilitated by a decoy receptor that can inactivate canonical cytokinin receptors via dimerization and compete with them for ligand binding. Ensuring proper plant growth and development requires precise control of the cytokinin signaling pathway at several levels. CRE1int7 represents a so-far unknown mechanism for fine-tuning the cytokinin signaling pathway in Arabidopsis.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {The potential applications of cytokinins and cytokinin oxidase/ dehydrogenase inhibitors for mitigating abiotic stresses in model and non-model plant species},

author = {Mxolisi P. Voko and Adeyemi O. Aremu and Nokwanda P. Makunga and Jaroslav Nisler and Karel Dolezal and Nqobile A. Masondo},

url = {10.1016/j.cpb.2024.100398

},

doi = {10.1016/j.cpb.2024.100398},

year  = {2024},

date = {2024-12-01},

journal = {CURRENT PLANT BIOLOGY},

volume = {40},

pages = {1-26},

abstract = {Cytokinins (CKs) are important phytohormones which are used by plants to optimize responses against abiotic stresses such as drought, salinity, temperature and nutrient stresses known to repress germination, and influencing general plant growth and development. Such stresses often trigger phenotypic plasticity and lead to low yields. Yet, the beneficial effect of CKs is counteracted by cytokinin oxidase/dehydrogenase (CKO/CKX, EC 1.5.99.12) enzymes and by N- and/or O-glycosylation. Additionally, research on CKs and CKX is often limited to model plants studied in isolation, and sparsely covers non-model plants exposed to abiotic stresses. Thus, this review explored the role of CKs and CKX inhibitors in mitigating abiotic stresses in model and non-model plants. We also examined possible crosstalk mechanisms of CKs with auxins, polyamines, and other major phytohormones. A detailed literature search was conducted using several databases including Web of Science, Google Scholar, ScienceDirect, Scopus, and PubMed. Upon perception of environmental stimuli, CKs [e.g., N6-(Delta 2isopent-2-enyl)adenine (iP), trans-zeatin (tZ) and cis-zeatin (cZ)] induce abiotic stress tolerance in a CK - dependent manner or by forming intermolecular pathways with abscisic acid, ethylene, auxins and polyamines. Regulatory motifs of type-B ARRs code for transcriptional responses via DNA-binding. Inhibitors of CKX (e.g., 3TFM-2HE, INCYDE, F-INCYDE and anisiflupurin) act as promoters of growth and stress-tolerance through the inhibition of catabolic CKXs and regulate an increase in endogenous CKs (e.g., iP, tZ and cZ) in plants. The ability of CKX inhibitors to intercept CKX gene regulation is an indication of their potential applications in agriculture and other industries that rely on plant-based products.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Comparative evaluation of the anti-proliferative effects of alkaloid-rich extract of jujube seed and paclitaxel on MDA-MB-231 breast cancer cell line},

author = {Emran Habibi and Amin Sepehrara and Hesamoddin Arabnozari and Fariborz Sharifianjazi and Seyed Ehsan Enderami and Satyajit D. Sarker and Hadi Hassannia and Lutfun Nahar},

url = {10.1016/j.jafr.2024.101438

},

doi = {10.1016/j.jafr.2024.101438},

issn = {2666-1543},

year  = {2024},

date = {2024-12-01},

journal = {JOURNAL OF AGRICULTURE AND FOOD RESEARCH},

volume = {18},

abstract = {Introduction: Cancer is the second cause of death in the world, and among all types of cancer, triple-negative breast cancer (TNBC) has one of the worst prognoses. Repeated use of chemotherapy drugs is associated with drug resistance. This study aims to investigate the effect of jujube alkaloid-rich extract on drug sensitivity and its anti-tumor effects on paclitaxel-resistant MDA-MB-231 cells in vitro. Materials and methods: The MDA-MB-231 breast cancer cell line and its paclitaxel-resistant variant were used. The XTT method was employed to measure cell viability, while Annexin-PI assays were used to detect apoptosis. Various concentrations of the jujube extract alone and in combination with paclitaxel assessed synergistic effects in 2D and 3D cell culture models. Results: The presence of alkaloids in the extract obtained from the jujube seed was confirmed by the dragendorff test and its amount was 0.395 g in 5.0 g of jujube seed powder. Paclitaxel and jujube extract exhibited dosedependent cytotoxic effects on paclitaxel-resistant MDA-MB-231 cells. The combination of jujube extract and paclitaxel significantly decreased IC50 from 541.3 mu g/mL to 124.3 mu g/mL, and the IC20 decreased from 92.1 mu g/ mL to 23.9 mu g/mL when combined with paclitaxel in 2D culture model. The combination's toxicity was reduced in the 3D culture model. Apoptosis rates were 0.75 % in the control group, 13.89 % in the alkaloid-rich extract group, 2.59 % in the paclitaxel group, and 42.90 % in the combination group. Conclusion: Combining paclitaxel with alkaloid-rich jujube seed extract enhances cytotoxicity and apoptosis in paclitaxel-resistant MDA-MB-231 breast cancer cells in both 2D and 3D culture models. This suggests that such combinations might be a viable strategy to overcome drug resistance in TNBC treatments.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Misincorporations of amino acids in p53 in human cells at artificially constructed termination codons in the presence of the aminoglycoside Gentamicin},

author = {Kamila Pawlicka and Tomas Henek and Lukas Uhrik and Lenka Hernychova and Monikaben Padariya and Jakub Faktor and Slawomir Makowiec and Borivoj Vojtesek and David Goodlett and Ted Hupp and Umesh Kalathiya},

url = {10.3389/fgene.2024.1407375

},

doi = {10.3389/fgene.2024.1407375},

year  = {2024},

date = {2024-11-05},

journal = {FRONTIERS IN GENETICS},

volume = {15},

abstract = {Readthrough of a translation termination codon is regulated by ribosomal A site recognition and insertion of near-cognate tRNAs. Small molecules exist that mediate incorporation of amino acids at the stop codon and production of full-length, often functional protein but defining the actual amino acid that is incorporated remains a challenging area. Herein, we report on the development a human cell model that can be used to determine whether rules can be developed using mass spectrometry that define the type of amino acid that is placed at a premature termination codon (PTC) during readthrough mediated by an aminoglycoside. The first PTC we analyzed contained the relatively common cancer-associated termination signal at codon 213 in the p53 gene. Despite of identifying a tryptic peptide with the incorporation of an R at codon 213 in the presence of the aminoglycoside, there were no other tryptic peptides detected across codon 213 that could be recovered; hence we constructed a more robust artificial PTC model. P53 expression plasmids were developed that incorporate a string of single synthetic TGA (opal) stop codons at S127P128A129 within the relatively abundant tryptic p53 peptide 121-SVTCTYSPALNK-132. The treatment of cells stably expressing the p53-TGA129 mutation, treated with Gentamicin, followed by immunoprecipitation and trypsinization of p53, resulted in the identification R, W, or C within the tryptic peptide at codon-TGA129; as expected based on the two-base pairing of the respective anticodons in the tRNA to UGA, with R being the most abundant. By contrast, incorporating the amber or ochre premature stop codons, TAA129 or TAG129 resulted in the incorporation of a Y or Q amino acid, again as expected based on the two base pairings to the anticodons, with Q being the most abundant. A reproducible non-canonical readthrough termination codon-skip event at the extreme C-terminus at codon 436 in the SBP-p53 fusion protein was detected which provided a novel assay for non-canonical readthrough at an extreme C-terminal PTC. The incorporation of amino acids at codons 127, 128, or 129 generally result in a p53 protein that is predicted to be 'unfolded' or inactive as defined by molecular dynamic simulations presumably because the production of mixed wild-type p53 and mutant oligomers are known to be inactive through dominant negative effects of the mutation. The data highlight the need to not only produce novel small molecules that can readthrough PTCs or C-terminal termination codons, but also the need to design methods to insert the required amino acid at the position that could result in a 'wild-type' functional protein.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Sucrose-responsive osmoregulation of plant cell size by a long non-coding RNA},

author = {Jakub Hajny and Tereza Travnickova and Martina Spundova and Michelle Roenspies and R. M. Imtiaz Karim Rony and Sebastian Sacharowski and Michal Krzyszton and David Zalabak and Christian S. Hardtke and Ales Pecinka and Holger Puchta and Szymon Swiezewski and Jaimie M. van Norman and Ondrej Novak},

url = {10.1016/j.molp.2024.09.011

},

doi = {10.1016/j.molp.2024.09.011},

issn = {1674-2052},

year  = {2024},

date = {2024-11-04},

journal = {MOLECULAR PLANT},

volume = {17},

issue = {11},

pages = {1719-1732},

abstract = {In plants, sugars are the key source of energy and metabolic building blocks. The systemic transport of sugars is essential for plant growth and morphogenesis. Plants evolved intricate molecular networks to effectively distribute sugars. The dynamic distribution of these osmotically active compounds is a handy tool for regulating cell turgor pressure, an instructive force in developmental biology. In this study, we have investigated the molecular mechanism behind the dual role of the receptor-like kinase CANAR. We functionally characterized a long non-coding RNA, CARMA, as a negative regulator of CANAR. Sugar- responsive CARMA specifically fine-tunes CANAR expression in the phloem, the route of sugar transport. Our genetic, molecular, microscopy, and biophysical data suggest that the CARMA-CANAR module controls the shoot-to-root phloem transport of sugars, allows cells to flexibly adapt to the external osmolality by appropriate water uptake, and thus adjust the size of vascular cell types during organ growth and development. Our study identifies a nexus of plant vascular tissue formation with cell internal pressure monitoring, revealing a novel functional aspect of long non-coding RNAs in developmental biology.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {BBX21 Integrates Brassinosteroid Biosynthesis and Signaling in the Inhibition of Hypocotyl Growth under Shade},

author = {Gabriel Gomez-Ocampo and Carlos D. Crocco and Jimena Cascales and Jana Oklestkova and Danuse Tarkowska and Miroslav Strnad and Santiago Mora-Garcia and Jose L. Pruneda-Paz and Miguel A. Blazquez and Javier F. Botto},

url = {10.1093/pcp/pcad126

},

doi = {10.1093/pcp/pcad126},

issn = {0032-0781},

year  = {2024},

date = {2024-11-01},

journal = {PLANT AND CELL PHYSIOLOGY},

volume = {65},

issue = {10},

pages = {1627-1639},

abstract = {B-Box-containing zinc finger transcription factors (BBX) are involved in light-mediated growth, affecting processes such as hypocotyl elongation in Arabidopsis thaliana. However, the molecular and hormonal framework that regulates plant growth through BBX proteins is incomplete. Here, we demonstrate that BBX21 inhibits the hypocotyl elongation through the brassinosteroid (BR) pathway. BBX21 reduces the sensitivity to 24-epiBL, a synthetic active BR, principally at very low concentrations in simulated shade. The biosynthesis profile of BRs showed that two active BR-brassinolide and 28-homobrassinolide-and 8 of 11 intermediates can be repressed by BBX21 under white light (WL) or simulated shade. Furthermore, BBX21 represses the expression of CYTOCHROME P450 90B1 (DWF4/CYP90B1), BRASSINOSTEROID-6-OXIDASE 1 (BR6OX1, CYP85A1) and BR6OX2 (CYP85A2) genes involved in the BR biosynthesis in WL while specifically promoting DWF4 and PHYB ACTIVATION TAGGED SUPPRESSOR 1 (CYP2B1/BAS1) expression in WL supplemented with far-red (WL + FR), a treatment that simulates shade. In addition, BBX21 represses BR signaling genes, such as PACLOBUTRAZOL RESISTANCE1 (PRE1), PRE3 and ARABIDOPSIS MYB-LIKE 2 (MYBL2), and auxin-related and expansin genes, such as INDOLE-3-ACETIC ACID INDUCIBLE 1 (IAA1), IAA4 and EXPANSIN 11 in short-term shade. By a genetic approach, we found that BBX21 acts genetically upstream of BRASSINAZOLE-RESISTANT 1 (BZR1) for the promotion of DWF4 and BAS1 gene expression in shade. We propose that BBX21 integrates the BR homeostasis and shade-light signaling, allowing the fine-tuning of hypocotyl elongation in Arabidopsis.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Phytochemical analysis and immune-modulatory potential of Trichaptum biforme polysaccharides: Implications for cancer},

author = {Emran Habibi and Parsa Hemmati and Hesamoddin Arabnozari and Hasti Asadi Khalili and Fariborz Sharifianjazi and Seyed Ehsan Enderami and Satyajit D. Sarker and Hadi Hassannia and Lutfun Nahar},

url = {10.1016/j.ijbiomac.2024.135691

},

doi = {10.1016/j.ijbiomac.2024.135691},

issn = {0141-8130},

year  = {2024},

date = {2024-11-01},

journal = {INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES},

volume = {280},

abstract = {Cancer remains a major global health concern, often challenging traditional treatments. Natural compounds like fungal polysaccharides have gained attention for their immune-modulatory properties. This study evaluates the phytochemical properties of the n-hexane fraction of Trichaptum biforme and explores its immune-enhancing effects. The study involved isolating three sterol derivatives using column chromatography and purifying polysaccharides from T. biforme (TBP) through hot aqueous extraction. TBP content was quantified via the phenol-sulfuric acid method, and antioxidant activity was assessed using DPPH and FRAP assays. Cytotoxicity of TBP on THP-1 cells and the impact on IL-1(3 and TNF-alpha secretion were evaluated through the XTT assay. Flow cytometry and ELISA assessed cytotoxic activity and IFN-gamma secretion in NK cells. The compound 9, 11-Dehydroergosterol peroxide was identified for the first time in T. biforme. . The total polysaccharide content was 78.18 +/- 0.81 %. The TBP significantly increased IL-1(3 and TNF-alpha secretion from THP-1 cells at concentrations of 10 and 320 mu g/mL (p p < 0.01). Treatment of NK cells with the extract (320 g/mL) and IL-2 (100 units/mL) significantly enhanced cytotoxic activity and IFN-gamma secretion compared to the control group (p < 0.01). These findings suggest that TBP holds promise as a candidate for bolstering anticancer immune responses.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Oleanolic acid purified from the stem bark of Olax subscorpioidea Oliv. inhibits the function and catalysis of human 17β-hydroxysteroid dehydrogenase 1},

author = {Yemi A. Adekunle and Babatunde B. Samuel and Wande M. Oluyemi and Adeniyi T. Adewumi and Salerwe Mosebi and Lutfun Nahar and Amos A. Fatokun and Satyajit D. Sarker},

url = {10.1080/07391102.2024.2423173},

doi = {10.1080/07391102.2024.2423173},

isbn = {0739-1102},

year  = {2024},

date = {2024-10-28},

journal = {JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS},

abstract = {Cancer is a leading cause of global death. Medicinal plants have gained increasing attention in cancer drug discovery. In this study, the stem bark extract of Olax subscorpioidea, which is used in ethnomedicine to treat cancer, was subjected to phytochemical investigation leading to the isolation of oleanolic acid (OA). The structure was elucidated by 1-dimensional and 2-dimensional nuclear magnetic resonance spectroscopic (NMR) data, and by comparing its data with previously reported data. Molecular docking was used to investigate the interactions of OA with nine selected cancer-related protein targets. OA docked well with human 17 beta-hydroxysteroid dehydrogenase type-1 (17 beta HSD1), caspase-3, and epidermal growth factor receptor tyrosine kinase (binding affinities: -9.8, -9.3, and -9.1 kcal/mol, respectively). OA is a triterpenoid compound with structural similarity to steroids. This similarity with the substrates of 17 beta HSD1 gives the inhibitor candidate an excellent opportunity to bind to 17 beta HSD1. The structural and functional dynamics of OA-17 beta HSD1 were investigated by molecular dynamics simulations at 240 ns. Molecular mechanics/Poisson-Boltzmann surface area (MMPBSA) studies showed that OA had a binding free energy that is comparable with that of vincristine (-52.76, and -63.56 kcal/mol, respectively). The average C-alpha root mean square of deviation (RMSD) value of OA (1.69 & Aring;) compared with the unbound protein (2.01 & Aring;) indicated its high stability at the protein's active site. The binding energy and stability at the active site of 17 beta HSD1 recorded in this study indicate that OA exhibited profound inhibitory potential. OA could be a good scaffold for developing new anti-breast cancer drugs.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {In Vitro Cytotoxicity and Antimicrobial Activity against Acne-Causing Bacteria and Phytochemical Analysis of Galangal (Alpinia galanga) and Bitter Ginger (Zingiber zerumbet) Extracts},

author = {Nongkhai, Tanat Na; Maddocks, Sarah E.; Phosri, Santi; Sangthong, Sarita; Pintathong, Punyawatt; Chaiwut, Phanuphong; Chandarajoti, Kasemsiri; Nahar, Lutfun; Sarker, Satyajit D.; Theansungnoen, Tinnakorn },

url = {10.3390/ijms252010869

},

doi = {10.3390/ijms252010869},

issn = {1661-6596},

year  = {2024},

date = {2024-10-10},

journal = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES},

volume = {25},

issue = {20},

abstract = {Galangal (Alpinia galanga (L.) Willd) and bitter ginger (Zingiber zerumbet (L.) Roscoe) are aromatic rhizomatous plants that are typically used for culinary purposes. These rhizomatous plants have many biological properties and the potential to be beneficial for pharmaceutics. In this study, we evaluated the antioxidant and antimicrobial activities, with a specific focus on acne-causing bacteria, as well as the phytochemical constituents, of different parts of galangal and bitter ginger. The rhizomes, stems, and leaves of galangal and bitter ginger were separately dried for absolute ethanol and methanol extractions. The extracts were used to evaluate the antioxidant activity using a DPPH radical scavenging assay (0.005-5000 mu g/mL), antimicrobial activity against acne-causing bacteria (0.50-31.68 mg/mL), and in vitro cytotoxicity toward human keratinocytes and fibroblasts (62.5-1000 mu g/mL), as well as analyses of bioactive phytochemicals via GC-MS and LC-MS/MS (500 ppm). The ethanol and methanol extracts of bitter ginger and galangal's rhizomes (BRhE, BRhM, GRhE, and GRhM), stems (BStE, BStM, GRhE, and GRhM), and leaves (BLeE, BLeM, GLeE, and GLeM), respectively, showed antioxidant and antimicrobial activities. The extracts of all parts of bitter ginger and galangal were greatly antioxidative with 0.06-1.42 mg/mL for the IC50 values, while most of the extracts were strongly antimicrobial against C. acnes DMST 14916, particularly BRhM, BRhE, GRhM, and GRhE (MICs: 3.96-7.92 mg/mL). These rhizome extracts had also antimicrobial activities against S. aureus TISTR 746 (MICs: 7.92-31.68 mg/mL) and S. epidermidis TISTR 518 (MICs: 7.92-15.84 mg/mL). The extracts of bitter ginger and galangal rhizomes were not toxic to HaCaT and MRC-5 even at the highest concentrations. Through GC-MS and LC-MS/MS analysis, phytochemicals in bitter ginger rhizome extracts, including zerumbone, tectorigenin, piperic acid, demethoxycurcumin, and cirsimaritin, and galangal rhizome extracts, including sweroside and neobavaisoflavone, were expected to provide the antioxidant and anti-microbial activities. Therefore, the results suggest that the bitter ginger and galangal extracts could be natural anti-acne compounds with potential for pharmaceutic, cosmetic, and aesthetic applications.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Emerging pharmacological approaches for Huntington's disease},

author = {Kuldeep Singh and Divya Jain and Pranshul Sethi and Jeetendra Kumar Gupta and Arpan Kumar Tripathi and Shivendra Kumar and Satyajit D. Sarker and Lutfun Nahar and Ajay Guru},

url = {10.1016/j.ejphar.2024.176873

},

doi = {10.1016/j.ejphar.2024.176873},

issn = {0014-2999},

year  = {2024},

date = {2024-10-05},

journal = {EUROPEAN JOURNAL OF PHARMACOLOGY},

volume = {980},

abstract = {Huntington's disease (HD) is a progressive neurodegenerative disorder characterized by cognitive, motor, and psychiatric symptoms. Despite significant advances in understanding the underlying molecular mechanisms of HD, there is currently no cure or disease-modifying treatment available. Emerging pharmacological approaches offer promising strategies to alleviate symptoms and slow down disease progression. This comprehensive review aims to provide a critical appraisal of the latest developments in pharmacological interventions for HD. The review begins by discussing the pathogenesis of HD, focusing on the role of mutant huntingtin protein, mitochondrial dysfunction, excitotoxicity, and neuro-inflammation. It then explores emerging therapeutic targets, including the modulation of protein homeostasis, mitochondrial function, neuro-inflammation, and neurotransmitter systems. Pharmacological agents targeting these pathways are discussed, including small molecules, gene-based therapies, and neuroprotective agents. In recent years, several clinical trials have been conducted to evaluate the safety and efficiency of novel compounds for HD. This review presents an update on the outcomes of these trials, highlighting promising results and challenges encountered. Additionally, it discusses the potential of repurposing existing drugs approved for other indications as a cost-effective approach for HD treatment. The review concludes by summarizing the current state of pharmacological approaches for HD and outlining future directions in drug development. The integration of multiple therapeutic strategies, personalized medicine approaches, and combination therapies are highlighted as potential avenues to maximize treatment effectiveness.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Novel neuroprotective 5,6-dihydropyrido[2′,1':2,3]imidazo[4,5-c] quinoline derivatives acting through cholinesterase inhibition and CB2 signaling modulation},

author = {Sushovan Jena and Gabriel Gonzalez and Dominik Vitek and Marie Kvasnicova and Sarka Stepankova and Miroslav Strnad and Jiri Voller and Kaushik Chanda},

url = {10.1016/j.ejmech.2024.116592

},

doi = {10.1016/j.ejmech.2024.116592},

issn = {0223-5234},

year  = {2024},

date = {2024-10-05},

journal = {EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY},

volume = {276},

abstract = {A novel group of 5,6-dihydropyrido [2 ',1':2,3]imidazo [4,5-c]quinolines was prepared via a microwave assisted one-pot telescopic approach. The synthetic sequence involves the formation of an amine precursor of imidazo [1,2-a]pyridine via condensation and reduction under microwave irradiation. Subsequently, the Pictet-Spengler cyclisation reaction occurs with ketones (cyclic or acyclic) to obtain substituted 5,6-dihydropyrido [2 ',1':2,3] imidazo [4,5-c]quinolines in excellent yields. The compounds were tested as neuroprotective agents. Observed protection of neuron-like cells, SH-SY5Y differentiated with ATRA, in Parkinson's and Huntington's disease models inspired further mechanistic studies of protective activity against damage induced by 1-methyl-4-phenylpyridinium (MPP+), a compound causing Parkinson's disease. The novel compounds exhibit similar or higher potency than ebselen, an established drug with antioxidant activity, in the cells against MPP + -induced total cellular superoxide production and cell death. However, they exhibit a significantly higher capacity to reduce mitochondrial superoxide and preserve mitochondrial membrane potential. We also observed marked differences between a selected derivative and ebselen in terms of normalizing MPP + -induced phosphorylation of Akt and ERK1/2. The cytoprotective activity was abrogated when signaling through cannabinoid receptor CB2 was blocked. The compounds also inhibit both acetylcholine and butyrylcholine esterases. Overall the data show that novel 5,6-dihydropyrido [2 ',1':2,3]imidazo [4,5-c]quinoline have a broad cytoprotective activity which is mediated by several mechanisms including mitoprotection.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {The Bioprotective Effects of Marigold Tea Polyphenols on Obesity and Oxidative Stress Biomarkers in High-Fat-Sugar Diet-Fed Rats},

author = {Bader Alsuwayt and Neelam Iftikhar and Abdullah Ijaz Hussain and Ashfaq Ahmad and Irsa Zafar and Arifa Khanam and Wen-Nee Tan and Lutfun Nahar and Afaf F. Almuqati and Esraa Mohammad Haji and Ali F. Almutairy and Satyajit D. Sarker},

url = {10.1155/2024/3833521

},

doi = {10.1155/2024/3833521},

issn = {1755-5914},

year  = {2024},

date = {2024-10-04},

journal = {CARDIOVASCULAR THERAPEUTICS},

abstract = {Background: The research is aimed at exploring the potential of marigold petal tea (MPT), rich in polyphenol contents, against oxidative stress and obesity in a rat model following a high-fat-sugar diet (HFSD).Methods: The MPT was prepared through the customary method of decoction and was subjected to analysis for its polyphenol composition using reversed-phase high-performance liquid chromatography (RP-HPLC). Two specific doses of MPT, namely, 250 and 500 mg/kg body weight (BW), were chosen for the study-referred to as MPT-250 and MPT-500, respectively.Result: The main phenolic acids and flavonoids identified in MPT, with concentrations exceeding 10 mg/100 mL of tea, included catechin, rutin, salicylic acid, gallic acid, sinapic acid, chlorogenic acid, cinnamic acid, and ellagic acid. The total phenolic (TP) and total flavonoid (TF) contents in MPT were measured to be 5.53 and 7.73 mg/g, respectively. Additionally, MPT demonstrated a 57.2% scavenging capacity with 2,2-diphenyl-1-picrylhydrazyl radical. Notably, the administration of a higher dose (MPT-500) showed a significant reduction in body mass index (BMI) and a 51.24% reduction in the rate of increase in BW compared to the HFSD group. The findings indicated that all the treatment groups, that is, orlistat treatment (OT), MPT-250, and MPT-500 groups, experienced reduced levels of serum total cholesterol (TC), triglyceride (TG), and markers of lipoproteins in contrast to the HFSD group. Moreover, MPT helped restore the levels of malondialdehyde (MDA), superoxide dismutase (SOD), and reduced glutathione (GSH), thereby demonstrating its potential in combating oxidative stress. The MPT-500 group also displayed decreased liver and kidney weights and an improved atherogenic index when compared to the HFSD group.Conclusion: The results clearly indicate that a high dosage of MPT showed antiobesity activity which was comparable to the same effects produced by the conventional drug orlistat.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Cytotoxic Flavonoids from Lannea egregia Engl. & K. Krause},

author = {Oluwatosin T. Ayodele and Olaoluwa O. Olaoluwa and Saheed O. Benson and Olapeju O. Aiyelaagbe and Lutfun Nahar and Amos A. Fatokun and Satyajit D. Sarker},

issn = {1735-403X},

year  = {2024},

date = {2024-10-01},

journal = {PHARMACEUTICAL SCIENCES},

volume = {30},

issue = {4},

pages = {496-501},

abstract = {Background: Lannea egregia Engl. & K. Krause (family: Anacardiaceae) is a well-known medicinal plant in Nigeria whose various parts have been shown to elicit several biological activities. This study specifically explored the leaf of L. egregia for potential cytotoxic compounds. Methods: n-Hexane, dichloromethane and methanolic extracts of the leaf were prepared using the Soxhlet apparatus and concentrated using the rotary evaporator. Compounds were isolated by reversed-phase preparative high-performance liquid chromatography, and the structures were determined by spectroscopic means. The methanolic extract and the isolated compounds were screened for cytotoxicity against HeLa and MCF-7 cancer cell lines, using the MTT assay. Results: Three flavonoids, myricetin (1), myricetin 3-O-alpha-L-rhamnoside (2) and quercetin 3-O-alpha-L-rhamnoside (3), were isolated from the methanolic extract of the leaf of L. egregia. The methanolic extract and compound 3 showed the most potent inhibition profiles against the cells, with IC50 values (Mean f SEM) of 45.3 f 1.5 mu g/mL and 57.5 f 0.4 mu g/mL for the methanolic extract, and 36.5 f 2.0 mu M and 57.9 f 10.1 mu M for compound 3, against HeLa and MCF-7 cells, respectively. Conclusion:

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Effects of Zinc Phthalocyanine Photodynamic Therapy on Vital Structures and Processes in Hela Cells},

author = {Jakub Hosik and Barbora Hosikova and Svatopluk Binder and Rene Lenobel and Marketa Kolarikova and Lukas Malina and Hanna Dilenko and Katerina Langova and Robert Bajgar and Hana Kolarova},

url = {10.3390/ijms251910650

},

doi = {10.3390/ijms251910650},

issn = {1661-6596},

year  = {2024},

date = {2024-10-01},

journal = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES},

volume = {25},

issue = {19},

abstract = {This work presents results on the efficiency of newly designed zinc phthalocyanine-mediated photodynamic therapy of both tumoral and nontumoral cell models using the MTT assay. Further detailed examinations of mechanistic and cell biological effects were focused on the HELA cervical cancer cell model. Here, ROS production, changes in the mitochondrial membrane potential, the determination of genotoxicity, and protein changes determined by capillary chromatography and tandem mass spectrometry with ESI were analyzed. The results showed that, in vitro, 5 Jcm-2 ZnPc PDT caused a significant increase in reactive oxygen species. Still, except for superoxide dismutase, the levels of proteins involved in cell response to oxidative stress did not increase significantly. Furthermore, this therapy damaged mitochondrial membranes, which was proven by a more than 70% voltage-dependent channel protein 1 level decrease and by a 65% mitochondrial membrane potential change 24 h post-therapy. DNA impairment was assessed by an increased level of DNA fragmentation, which might be related to the decreased level of DDB1 (decrease in levels of more than 20% 24 h post-therapy), a protein responsible for maintaining genomic integrity and triggering the DNA repair pathways. Considering these results and the low effective concentration (LC50 = 30 nM), the therapy used is a potentially very promising antitumoral treatment.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {In Vitro Interaction of Binuclear Copper Complexes with Liver Drug-Metabolizing Cytochromes P450},

author = {Alena Spicakova and Zuzana Horackova and Pavel Kopel and Pavel Anzenbacher},

url = {10.3390/ph17091194

},

doi = {10.3390/ph17091194},

issn = {1424-8247},

year  = {2024},

date = {2024-09-10},

urldate = {2024-09-10},

journal = {PHARMACEUTICALS},

volume = {17},

issue = {9},

pages = {1-11},

abstract = {Two copper(II) mixed ligand complexes with dicarboxylate bridges were prepared and studied, namely [Cu-2(mu-fu)(pmdien)(2)(H2O)(2)](ClO4)(2) (complex No. 5) and [Cu-2(mu-dtdp)(pmdien)(2)(H2O)(2)](ClO4)(2) (complex No. 6), where H(2)fu = fumaric acid, pmdien = N,N,N ',N '',N '' pentamethyldiethylenetriamine, and H(2)dtdp = 3,3 '-dithiodipropionic acid. The copper atoms are coordinated in the same mode by the tridentate pmdien ligand and oxygen of water molecules, and they only differ in the dicarboxylate bridge. This work is focused on the study of the inhibitory effect of these potential antimicrobial drugs on the activity of the most important human liver drug-metabolizing enzymes, cytochromes P450 (CYP), especially their forms CYP2C8, CYP2C19, and CYP3A4. The obtained results allow us to estimate the probability of potential drug interactions with simultaneously administrated drugs that are metabolized by these CYP enzymes. In conclusion, the presence of adverse effects due to drug-drug interactions with concomitantly used drugs cannot be excluded, and hence, topical application may be recommended as a relatively safe approach.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Phytochemistry and therapeutic potential of the genus Asphodelus L.: an update},

author = {Lutfun Nahar and Afaf Al Groshi and Lakshmi Thangavelu and Fyaz M. D. Ismail and Andrew R. Evans and Satyajit D. Sarker},

url = {10.1007/s11101-024-10004-6

},

doi = {10.1007/s11101-024-10004-6},

issn = {1568-7767},

year  = {2024},

date = {2024-09-06},

journal = {PHYTOCHEMISTRY REVIEWS},

abstract = {The genus Asphodelus L. (family: Asphodelaceae) comprises ca. 20 hardy herbaceous, perennial flowering plant species, and is native to Africa, the Indian subcontinent, the Mediterranean region, and temperate Europe, and naturalized in Australia, Mexico, New Zealand, and some parts of the USA. The Asphodelus species have long been used as traditional medicines for the treatment of acne, alopecia, burns, earache, eczema, local inflammation, psoriasis, and toothache. Since the publication of the last review in 2019, which essentially covered the published literature until the end of 2018, several articles have been published reporting the identification of various secondary metabolites including anthraquinones, flavonoids and phenolic acids and demonstrating the therapeutic potential of the genus Asphodelus. This review article critically appraises the literature on this genus published during 2019-2023 on therapeutic potential and phytochemistry. During this period, among the identified compounds from various Asphodelus species, including A. aestivus Brot., A. albus Mill., A. bento-rainhae P. Silva, A. fistulosus L., A. macrocarpus Salzm. Viv., A. ramosus L. and A. tenuifolius Cav., the phytochemicals of the classes of anthraquinones, flavonoids and phenolic acids were the most dominating ones. Numerous studies established the therapeutic potential of the Asphodelus species mainly against cancer, diabetes, microbial infections, and various ailments caused by oxidative stress. None or negligible toxicity could be observed in the toxicological studies, suggesting an acceptable level of safety of Asphodelus products for potential therapeutic applications.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Caffeine-Legal Natural Stimulant with Open Research Perspective: Spectroscopic and Theoretical Characterization},

author = {Teobald Kupka and Natalina Makieieva and Michal Jewginski and Magdalena Witek and Barbara Blicharska and Oimahmad Rahmonov and Karel Dolezal and Tomas Pospisil},

url = {10.3390/molecules29184382

},

doi = {10.3390/molecules29184382},

issn = {1420-3049},

year  = {2024},

date = {2024-09-01},

journal = {MOLECULES},

volume = {29},

issue = {18},

abstract = {Caffeine is an alkaloid with a purine structure and has been well known for centuries due to its presence in popular drinks-tea and coffee. However, the structural and spectroscopic parameters of this compound, as well as its chemical and biological activities, are still not fully known. In this study, for the first time, we report on the measured oxygen-17 NMR spectra of this stimulant. To support the assignment of our experimental NMR data, extensive quantum chemical calculations of NMR parameters, including nuclear magnetic shielding constants and indirect spin-spin coupling constants, were performed. In a theoretical study, using nine efficient density functionals (B3LYP, BLYP, BP86, CAM-B3LYP, LC-BLYP, M06, PBE0, TPSSh, wB97x), and in combination with a large and flexible correlation-consistent aug-cc-pVTZ basis set, the structure and NMR parameters were predicted for a free molecule of caffeine and in chloroform, DMSO and water. A polarized continuum model (PCM) was used to include a solvent effect. As a result, an optimal methodology was developed for predicting reliable NMR data, suitable for studies of known, as well as newly discovered, purines and similar alkaloids. The results of the current work could be used in future basic and applied studies, including NMR identification and intermolecular interactions of caffeine in various raw materials, like plants and food, as well as in the structural and spectroscopic characterization of new compounds with similar structures.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Atropisomeric 1-phenylbenzimidazoles affecting microtubule organization: influence of axial chirality},

author = {Jana Pospisilova and Tomas Heger and Ondrej Kurka and Marie Kvasnicova and Anna Chladkova and Ivan Nemec and Lucie Rarova and Petr Cankar},

url = {10.1039/d4ob00863d},

doi = {10.1039/d4ob00863d},

issn = {1477-0520},

year  = {2024},

date = {2024-08-28},

journal = {ORGANIC & BIOMOLECULAR CHEMISTRY},

volume = {22},

issue = {34},

abstract = {Benzimidazoles are frequently used in medicinal chemistry. Their anticancer effect is among the most prominent biological activities exhibited by this scaffold. Although numerous benzimidazole derivatives have been synthesized, possible atropisomerism of ortho-substituted 1-phenylbenzimidazoles has been largely overlooked. The aim of this research was to synthesize a small library of novel atropisomeric benzimidazole derivatives and explore their biological activity in various cancer and normal human cell lines. The new unique structural motif provides an interesting 3D architecture with axial chirality, which further contributes to molecular complexity and specificity. Racemates and their separated atropisomers arrested the cell cycle, caused apoptosis, and affected microtubule organization in cancer cells in vitro at different intensities. Moreover, this phenomenon was also verified by the inhibition of endothelial cell migration. These results showed that (+)-atropisomers, especially 5n, exhibit a stronger effect and show promise as agents for cancer therapy. Axially chiral benzimidazoles affects microtubule organization.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {AAO2 impairment enhances aldehyde detoxification by AAO3 in Arabidopsis leaves exposed to UV-C or Rose-Bengal},

author = {Zhadyrassyn Nurbekova and Sudhakar Srivastava and Zai Du Nja and Kusum Khatri and Jaykumar Patel and Babita Choudhary and Veronica Tureckova and Miroslav Strand and Edyta Zdunek-Zastocka and Rustem Omarov and Dominic Standing and Moshe Sagi},

url = {10.1111/tpj.16985

},

doi = {10.1111/tpj.16985},

issn = {0960-7412},

year  = {2024},

date = {2024-08-27},

journal = {PLANT JOURNAL},

volume = {120},

issue = {1},

pages = {272-288},

abstract = {Among the three active aldehyde oxidases in Arabidopsis thaliana leaves (AAO1-3), AAO3, which catalyzes the oxidation of abscisic-aldehyde to abscisic-acid, was shown recently to function as a reactive aldehyde detoxifier. Notably, aao2KO mutants exhibited less senescence symptoms and lower aldehyde accumulation, such as acrolein, benzaldehyde, and 4-hydroxyl-2-nonenal (HNE) than in wild-type leaves exposed to UV-C or Rose-Bengal. The effect of AAO2 expression absence on aldehyde detoxification by AAO3 and/or AAO1 was studied by comparing the response of wild-type plants to the response of single-functioning aao1 mutant (aao1S), aao2KO mutants, and single-functioning aao3 mutants (aao3Ss). Notably, aao3Ss exhibited similar aldehyde accumulation and chlorophyll content to aao2KO treated with UV-C or Rose-Bengal. In contrast, wild-type and aao1S exhibited higher aldehyde accumulation that resulted in lower remaining chlorophyll than in aao2KO leaves, indicating that the absence of active AAO2 enhanced AAO3 detoxification activity in aao2KO mutants. In support of this notion, employing abscisic-aldehyde as a specific substrate marker for AAO3 activity revealed enhanced AAO3 activity in aao2KO and aao3Ss leaves compared to wild-type treated with UV-C or Rose-Bengal. The similar abscisic-acid level accumulated in leaves of unstressed or stressed genotypes indicates that aldehyde detoxification by AAO3 is the cause for better stress resistance in aao2KO mutants. Employing the sulfuration process (known to activate aldehyde oxidases) in wild-type, aao2KO, and molybdenum-cofactor sulfurase (aba3-1) mutant plants revealed that the active AAO2 in WT employs sulfuration processes essential for AAO3 activity level, resulting in the lower AAO3 activity in WT than AAO3 activity in aao2KO.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {A New Abscisic Acid Conjugate, ABA-L-Glutamate, Determined in Different Plant Species by Combined Immunoaffinity Chromatography-Tandem Mass Spectrometry},

editor = {Veronika Tureckova and Jana Oklestkova and Asta Zukauskaite and Ludek Eyer and Ondrej Novak and Miroslav Strnad},

url = {10.1007/s00344-024-11436-2

},

doi = {10.1007/s00344-024-11436-2},

issn = {0721-7595},

year  = {2024},

date = {2024-08-21},

journal = {JOURNAL OF PLANT GROWTH REGULATION},

volume = {43},

pages = {4810-4825},

abstract = {Abscisic acid (ABA) is a phytohormone that occurs in plants at very low concentration (pmol/g fresh weight) and regulates multiple biological processes, including stomatal closure, seed germination, and responses to environmental stresses. In the present study, isolation of ABA, ABA glucosyl ester, and 11 ABA amino acid conjugates from minute quantities of plant tissue (less than 20 mg fresh weight) was achieved using a purification method based on the combination of an Oasis HLB column and an immunoaffinity sorbent. New monoclonal antibodies raised against (+)-cis,trans-ABA conjugated to BSA through its carboxyl group (C1) were characterised by enzyme-linked immunosorbent assay (ELISA) and used for immunoaffinity chromatography (IAC) gel preparation. The use of immunoaffinity purification significantly reduced matrix effects and increased the selectivity and sensitivity of subsequent UHPLC-MS/MS analysis. In addition to (+)-cis,trans-ABA and its glucosyl ester, a new abscisic acid conjugate, ABA-L-glutamate, was isolated by IAC and identified by tandem mass spectrometry in pea (Pisum sativum L.), Lepidium sativum L. and wheat (Triticum aestivum L.) seedlings. However, it was not found in 10-day-old seedlings of Arabidopsis thaliana or water-stressed tobacco (Nicotiana tabacum L.) leaves. Here, the identification of an ABA conjugate with glutamic acid in plants is described for the first time.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {The effect of Polygonum hyrcanicum Rech. f. hydroalcoholic extract on oxidative stress and nephropathy in alloxan-induced diabetic mice},

author = {Aday Amirbekov and Stanislava Vrchovecka and Jakub Riha and Ivan Petrik and David Friedecky and Ondrej Novak and Miroslav Cernik and Pavel Hrabak and Alena Sevcu},

url = {10.1038/s41598-024-69220-x},

doi = {10.1038/s41598-024-69220-x},

issn = {2045-2322},

year  = {2024},

date = {2024-08-05},

journal = {SCIENTIFIC REPORTS},

volume = {14},

issue = {1},

abstract = {Diabetic nephropathy, characterized by inflammation and oxidative stress, poses a management challenge. This study investigates the effect of Polygonum hyrcanicum extract on diabetic nephropathy in alloxan-induced diabetic mice. In this experimental animal study, the P. hyrcanicum extract was prepared using continuous macerations. Thirty male Albino mice, divided into five groups, were induced with alloxan-induced diabetes. They received intraperitoneal injections of the plant extract (100 and 200 mg/kg) and metformin (300 mg/kg) for four weeks. Kidney and blood samples were collected to assess protein carbonyl, glutathione, lipid peroxidation, TNF-alpha and IL-6 levels. The amount of total flavonoid and phenolic content in the hydroalcoholic extract of P. hyrcanicum were 7.5 +/- 0.3 mg of quercetin and 88.2 +/- 1.3 mg gallic acid per gram of extract respectively. The antioxidant activity level of the hydroalcoholic extract was determined to be 1.78 +/- 0.51 mM equivalent per gram of extract. Alloxan administration resulted in a significant reduction in glutathione levels and a significant increase in protein carbonyl, lipid peroxidation, TNF-alpha, and IL-6 levels. Hydroalcoholic extract of P. hyrcanicum effectively reduced oxidative stress markers and inflammatory cytokines (TNF-alpha, IL-6), indicating its potential in mitigating diabetic nephropathy. However, no significant difference in efficacy was observed between the 100 mg/kg and 200 mg/kg doses in terms of reducing these toxicities.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Systemic strategies for cytokinin biosynthesis and catabolism in Arabidopsis roots and leaves under prolonged ammonium nutrition},

author = {Kacper Dziewit and Petra Amakorova and Ondrej Novak and Bozena Szal and Anna Podgorska},

url = {10.1016/j.plaphy.2024.108858

},

doi = {10.1016/j.plaphy.2024.108858},

issn = {0981-9428},

year  = {2024},

date = {2024-08-01},

journal = {PLANT PHYSIOLOGY AND BIOCHEMISTRY},

volume = {213},

abstract = {Cytokinins are growth -regulating plant hormones that are considered to adjust plant development under environmental stresses. During sole ammonium nutrition, a condition known to induce growth retardation of plants, altered cytokinin content can contribute to the characteristic ammonium toxicity syndrome. To understand the metabolic changes in cytokinin pools, cytokinin biosynthesis and degradation were analyzed in the leaves and roots of mature Arabidopsis plants. We found that in leaves of ammonium -grown plants, despite induction of biosynthesis on the expression level, there was no active cytokinin build-up because they were effectively routed toward their downstream catabolites. In roots, cytokinin conjugation was also induced, together with low expression of major synthetic enzymes, resulting in a decreased content of the trans -zeatin form under ammonium conditions. Based on these results, we hypothesized that in leaves and roots, cytokinin turnover is the major regulator of the cytokinin pool and does not allow active cytokinins to accumulate. A potent negative -regulator of root development is trans -zeatin, therefore its low level in mature root tissues of ammonium -grown plants may be responsible for occurrence of a wide root system. Additionally, specific cytokinin enhancement in apical root tips may evoke a short root phenotype in plants under ammonium conditions. The ability to flexibly regulate cytokinin metabolism and distribution in root and shoot tissues can contribute to adjusting plant development in response to ammonium stress.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Karrikinolide1 (KAR1), a Bioactive Compound from Smoke, Improves the Germination of Morphologically Dormant Apium graveolens L. Seeds by Reducing Indole-3-Acetic Acid (IAA) Levels},

author = {Shubhpriya Gupta and Jakub Hrdlicka and Manoj Kulkarni and Ivana Dolezalova and Ales Pencik and Johannes Van Staden and Ondrej Novak and Karel Dolezal},

url = {10.3390/plants13152096

},

doi = {10.3390/plants13152096},

issn = {2223-7747},

year  = {2024},

date = {2024-08-01},

journal = {PLANTS-BASEL},

volume = {13},

issue = {15},

abstract = {Smoke-water (SW) and Karrikinolide1 (KAR(1)) release dormancy and improve seed germination in many plant species. Therefore, we tested SW (1:2500 v/v) and KAR(1) (10(-7) M) to break the morphological dormancy of celery cultivar (Apium graveolens L.). In the first trial, seeds were subjected to a 21-day incubation period at 20 degrees C with SW and KAR(1) applied as single treatments. KAR(1) showed significantly improved germination (30.7%) as compared to SW (17.2%) and a water control (14.7%). In seed soaking experiments, SW, KAR(1), and gibberellic acid (GA(3)) treatments showed higher germination percentages than the water control after 3 and 6 h of soaking. However, prolonged soaking (12 h) reduced germination percentages for all treatments, indicating a detrimental effect. Analysis of KAR(1) content dynamics in 7-day- and 21-day-old celery seeds indicated its prolonged effects on germination and dormancy alleviation. Phytohormones, including auxins in 7-day-old and cytokinins in 7-day- and 21-day-old celery seedlings, along with their precursors and metabolites, were analyzed using ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) after treatment with KAR(1) and SW. The analysis of auxin levels in 7-day-old seeds revealed a negative correlation between seed germination and auxin (indole-3-acetic acid, IAA) content. Notably, it was found that KAR(1)-treated seeds significantly reduced IAA levels in all treatments. SW and KAR(1) did not significantly affect cytokinin levels during celery germination except for N6-Isopentenyladenine. Hence, further research is needed to understand their precise role in celery seed germination. This work will improve our understanding of the role of bioactive compounds from plant-derived smoke and how they regulate hormonal responses and improve germination efficiency in celery.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{,

title = {Spatio-temporal plant hormonomics: from tissue to subcellular resolution},

author = {Ivan Petrik and Pavel Hladik and Chao Zhang and Ales Pencik and Ondrej Novak},

url = {10.1093/jxb/erae267},

doi = {10.1093/jxb/erae267},

issn = {0022-0957},

year  = {2024},

date = {2024-07-22},

urldate = {2024-01-01},

journal = {JOURNAL OF EXPERIMENTAL BOTANY},

volume = {75},

number = {5295},

issue = {17},

pages = {5311},

abstract = {Due to technological advances in mass spectrometry, significant progress has been achieved recently in plant hormone research. Nowadays, plant hormonomics is well established as a fully integrated scientific field focused on the analysis of phytohormones, mainly on their isolation, identification, and spatiotemporal quantification in plants. This review represents a comprehensive meta-study of the advances in the phytohormone analysis by mass spectrometry over the past decade. To address current trends and future perspectives, Web of Science data were systematically collected and key features such as mass spectrometry-based analyses were evaluated using multivariate data analysis methods. Our findings showed that plant hormonomics is currently divided into targeted and untargeted approaches. Both aim to miniaturize the sample, allowing high-resolution quantification to be covered in plant organs as well as subcellular compartments. Therefore, we can study plant hormone biosynthesis, metabolism, and signalling at a spatio-temporal resolution. Moreover, this trend has recently been accelerated by technological advances such as fluorescence-activated cell sorting or mass spectrometry imaging. A comprehensive meta-study was conducted to reveals research trends in plant hormone determination using targeted and untargeted metabolomic approaches published in the last decade.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Cytokinin oxidase/dehydrogenase inhibitors: progress towards agricultural practice},

author = {Jaroslav Nisler and Pavel Klimes and Radka Koncitikova and Alena Kadlecova and Jiri Voller and Mahfam Chalaki and Michael Karampelias and Nino Murvanidze and Stefaan P. O. Werbrouck and David Kopecny and Libor Havlicek and Nuria De Diego and Pierre Briozzo and Solange Morera and David Zalabak and Lukas Spichal},

url = {10.1093/jxb/erae239

},

doi = {10.1093/jxb/erae239},

issn = {0022-0957},

year  = {2024},

date = {2024-07-10},

urldate = {2024-07-10},

journal = {JOURNAL OF EXPERIMENTAL BOTANY},

volume = {74},

issue = {16},

pages = {4873-3890},

abstract = {Cytokinin oxidase/dehydrogenase (CKX) inhibitors reduce the degradation of cytokinins in plants and thereby may improve the efficiency of agriculture and plant tissue culture-based practices. Here, we report a synthesis and structure-activity relationship study of novel urea derivatives concerning their CKX inhibitory activity. The most active compounds showed sub-nanomolar IC50 values with maize ZmCKX1, the lowest value yet documented. Other CKX isoforms of maize and Arabidopsis were also inhibited very effectively. The binding mode of four compounds was characterized based on high-resolution crystal complex structures. Using the soil nematode Caenorhabditis elegans, and human skin fibroblasts, key CKX inhibitors with low toxicity were identified. These compounds enhanced the shoot regeneration of Lobelia, Drosera, and Plectranthus, as well as the growth of Arabidopsis and Brassica napus. At the same time, a key compound (identified as 82) activated a cytokinin primary response gene, ARR5:GUS, and a cytokinin sensor, TCSv2:GUS, without activating the Arabidopsis cytokinin receptors AHK3 and AHK4. This strongly implies that the effect of compound 82 is due to the up-regulation of cytokinin signalling. Overall, this study identifies highly effective and easily prepared CKX inhibitors with a low risk of environmental toxicity for further investigation of their potential in agriculture and biotechnology. This structure-activity relationship study of a new series of cytokinin oxidase/dehydrogenase inhibitors demonstrates compounds with very high efficacy, low toxicity, and high practical potential for agricultural use.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Metabolism of Fluorinated Topolin Cytokinins in Micropropagated Phalaenopsis amabilis},

author = {Nino Murvanidze and Karel Dolezal and Lenka Plackova and Stefaan P. O. Werbrouck},

url = {10.3390/horticulturae10070727

},

doi = {10.3390/horticulturae10070727},

issn = {2311-7524},

year  = {2024},

date = {2024-07-10},

journal = {HORTICULTURAE},

volume = {10},

issue = {7},

abstract = {Fluorinated cytokinins have emerged as promising alternatives to traditional cytokinins in Phalaenopsis plant tissue culture, offering enhanced stability and bioactivity. However, their metabolic fate and impact on endogenous cytokinin profiles remain largely unexplored. This study builds upon previous research to investigate the comparative metabolism of the traditional cytokinin 6-Benzylaminopurine (BA) with the successful alternatives 6-(3-fluorobenzylamino)purine (FmT) and 6-(3-fluorobenzylamino)purine 9-riboside (FmTR). Additionally, this study examines the impact of another crucial factor, the use of ventilated versus closed containers, on metabolic processes. The results revealed the distinct metabolic profiles associated with each treatment, highlighting the complex interplay between exogenous and endogenous cytokinin levels. This study is the first to investigate the effects of these stable, synthetic, and exogenous cytokinins on the naturally occurring cytokinin levels and their metabolites in micropropagated Phalaenopsis. Additionally, we proposed an alternative inactivation pathway involving the conversion of FmTR and BA to pT and pTR. These findings provide valuable insights into the intricate relationship between cytokinin metabolism and plant growth under in vitro conditions.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Cell wall integrity modulates HOOKLESS1 and PHYTOCHROME INTERACTING FACTOR4 expression controlling apical hook formation},

author = {Riccardo Lorrai and Ozer Erguvan and Sara Raggi and Kristoffer Jonsson and Jitka Siroka and Danuse Tarkowska and Ondrej Novak and Jayne Griffiths and Alexander M. Jones and Stephane Verger and Stephanie Robert and Simone Ferrari},

url = {10.1093/plphys/kiae370

},

doi = {10.1093/plphys/kiae370},

issn = {0032-0889},

year  = {2024},

date = {2024-07-08},

urldate = {2024-07-08},

journal = {PLANT PHYSIOLOGY},

volume = {196},

issue = {2},

pages = {1562–1578},

abstract = {Formation of the apical hook in etiolated dicot seedlings results from differential growth in the hypocotyl apex and is tightly controlled by environmental cues and hormones, among which auxin and gibberellins (GAs) play an important role. Cell expansion is tightly regulated by the cell wall, but whether and how feedback from this structure contributes to hook development are still unclear. Here, we show that etiolated seedlings of the Arabidopsis (Arabidopsis thaliana) quasimodo2-1 (qua2) mutant, defective in pectin biosynthesis, display severe defects in apical hook formation and maintenance, accompanied by loss of asymmetric auxin maxima and differential cell expansion. Moreover, qua2 seedlings show reduced expression of HOOKLESS1 (HLS1) and PHYTOCHROME INTERACTING FACTOR4 (PIF4), which are positive regulators of hook formation. Treatment of wild-type seedlings with the cellulose inhibitor isoxaben (isx) also prevents hook development and represses HLS1 and PIF4 expression. Exogenous GAs, loss of DELLA proteins, or HLS1 overexpression partially restore hook development in qua2 and isx-treated seedlings. Interestingly, increased agar concentration in the medium restores, both in qua2 and isx-treated seedlings, hook formation, asymmetric auxin maxima, and PIF4 and HLS1 expression. Analyses of plants expressing a F & ouml;rster resonance energy transfer-based GA sensor indicate that isx reduces accumulation of GAs in the apical hook region in a turgor-dependent manner. Lack of the cell wall integrity sensor THESEUS 1, which modulates turgor loss point, restores hook formation in qua2 and isx-treated seedlings. We propose that turgor-dependent signals link changes in cell wall integrity to the PIF4-HLS1 signaling module to control differential cell elongation during hook formation. Loss of cell wall integrity suppresses gibberellic acid accumulation and HOOKLESS1 and PHYTOCHROME INTERACTING FACTOR4 expression, ultimately repressing apical hook formation in Arabidopsis.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Long days induce adaptive secondary dormancy in the seeds of the Mediterranean plant Aethionema arabicum},

author = {Zsuzsanna Meral and Kai Graeber and Fei Xu and Mattia Dona and Katarina Lalatovic and Per K. I. Wilhelmsson and Noe Fernandez-Pozo and Stefan A. Rensing and Gerhard Leubner-Metzger and Ortrun Mittelsten Scheid and Liam Dolan},

url = {10.1016/j.cub.2024.05.043

},

doi = {10.1016/j.cub.2024.05.043},

issn = {0960-9822},

year  = {2024},

date = {2024-07-08},

journal = {CURRENT BIOLOGY},

volume = {34},

issue = {13},

abstract = {Secondary dormancy is an adaptive trait that increases reproductive success by aligning seed germination with permissive conditions for seedling establishment. Aethionema arabicum is an annual plant and member of the Brassicaceae that grows in environments characterized by hot and dry summers. Aethionema arabicum seeds may germinate in early spring when seedling establishment is permissible. We demonstrate that long-day light regimes induce secondary dormancy in the seeds of Aethionema arabicum (CYP accession), repressing germination in summer when seedling establishment is riskier. Characterization of mutants screened for defective secondary dormancy demonstrated that RGL2 mediates repression of genes involved in gibberellin (GA) signaling. Exposure to high temperature alleviates secondary dormancy, restoring germination potential. These data are consistent with the hypothesis that long-day-induced secondary dormancy and its alleviation by high temperatures may be part of an adaptive response limiting germination to conditions permissive for seedling establishment in spring and autumn.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Anogeissus leiocarpus (DC.) Guill. & Perr. (Combretaceae): A review of the traditional uses, phytochemistry and pharmacology of African birch},

author = {Yemi A. Adekunle and Babatunde B. Samuel and Lutfun Nahar and Amos A. Fatokun and Satyajit D. Sarker },

url = {10.1016/j.fitote.2024.105979

},

doi = {10.1016/j.fitote.2024.105979},

issn = {0367-326X},

year  = {2024},

date = {2024-07-01},

urldate = {2024-07-01},

journal = {FITOTERAPIA},

volume = {176},

abstract = {Anogeissus leiocarpus (DC.) Guill. & Perr. belongs to the family Combretaceae and is used both by African traditional medical practitioners and livestock rearers to treat diseases such as African trypanosomiasis, animal diarrhoea, asthma, cancer, cough, diabetes, dysentery, erectile dysfunction, fever, giardiasis, helminthiases, meningitis, menstrual disorders, monkeypox, oral infections, poliomyelitis, sickle cell anaemia, snake bites, toothache, urinary schistosomiasis, and yellow fever. Some of these activities have been associated with the presence of polyphenols in the plant which include ellagic acid derivatives, flavonoids, stilbenes, tannins, and triterpenes. Several bioactive molecules have been identified from A. leiocarpus . These include the main active constituents, ellagitannins, ellagic acid derivates, flavonoids and triterpenes. Pharmacological studies have confirmed its antibacterial, antifungal, antihyperglycemic, antihypertensive, antimalarial, antioxidative, antiparasitic, antitumour and anti -ulcer effects. The stem bark has been investigated mainly for biological activities and phytochemistry, and it is the most mentioned plant part highlighted by the traditional users in ethnomedicinal surveys. In vitro and in vivo models, which revealed a wide range of pharmacological actions against parasites causing helminthiasis, leishmaniasis, malaria and trypanosomiasis, have been used to study compounds from A. leiocarpus . Because of its uses in African traditional medicine and veterinary practices, A. leiocarpus has received considerable attention from researchers. The current review provides a comprehensive overview and critical appraisal of scientific reports on A. leiocarpus, covering its traditional uses, pharmacological activities and phytochemistry.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {A comprehensive review of the botany, ethnopharmacology, phytochemistry, and pharmacological activities of two Iranian Rydingia species (Lamiaceae)},

author = {Aminallah Tahmasebi and Babak Jamali and Vahideh Atabaki and Satyajit D. Sarker and Lutfun Nahar and Hye Jung Min and Chul Won Lee},

url = {10.1016/j.fitote.2024.106026

},

doi = {10.1016/j.fitote.2024.106026},

issn = {0367-326X},

year  = {2024},

date = {2024-07-01},

journal = {FITOTERAPIA},

volume = {176},

abstract = {Rydingia michauxii and R. persica, respectively, known as Kase Gol and Goldar in Persian, belong to the family Lamiaceae and they are well known herbal medicine in Iran for the treatment of various diseases, particularly diabetes. This review aims to appraise the phytochemistry, ethnopharmacology, and pharmacological activities of Rydingia species growing in Iran and assess their potential in clinical applications. Besides, it critically evaluates existing literature and looks into the perspective for further research and utilization. All available scientific literature was consulted using the database searches involving Google Scholar, PubMed, and Web of Science applying the keyword Rydingia and its Syn; Otostegia. Only the search results that are associated with the Iranian species R. michauxii and R. persica are included in this review. alpha-pinene, carvacrol, caryophyllene oxide, diisooctyl phthalate, dillapiole, eugenol, hexadecanoic acid, and pentacosane are the major constituents of the essential oils of the Rydingia species. Additionally, these species produce bioactive flavonoids, phenolic acids, steroids, and terpenoids. Extracts and active compounds from Rydingia species have been reported to possess various pharmacological activities including antidiabetic, anti-inflammatory, antimalarial, antimicrobial, antioxidant, cytotoxic, and lipid-lowering properties. Based on the information available to date on the Iranian Rydingia species, it will be worth subjecting these species to further developmental work involving preclinical and clinical trials.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Spider mite infestation triggers coordinated hormonal trade-offs enabling plant survival with a fitness cost},

author = {Alejandro Garcia and Lucia Talavera-Mateo and Ivan Petrik and Jana Oklestkova and Ondrej Novak and M. Estrella Santamaria},

url = {10.1111/ppl.14479

},

doi = {10.1111/ppl.14479},

issn = {0031-9317},

year  = {2024},

date = {2024-07-01},

journal = {PHYSIOLOGIA PLANTARUM},

volume = {176},

issue = {4},

abstract = {Tetranychus urticae is an important pest that causes severe damage to a wide variety of plants and crops, leading to a substantial productivity loss. Previous research has been focused on plant defence response to T. urticae to improve plant resistance. However, plant growth, development and reproduction throughout the infestation process have not been previously studied. Through physiological, biochemical, transcriptomic and hormonomic evaluation, we uncover the molecular mechanisms directing the defence-growth trade-off established in Arabidopsis upon T. urticae infestation. Upon mite attack, plants suffer an adaptation process characterized by a temporal separation between the defence and growth responses. Jasmonic and salicylic acids regulate the main defence responses in combination with auxin and abscisic acid. However, while the reduction of both auxin signalling and gibberellin, cytokinin and brassinosteroid biosynthesis lead to initial growth arrest, increasing levels of growth hormones at later stages enables growth restart. These alterations lead to a plant developmental delay that impacts both seed production and longevity. We demonstrate that coordinated trade-offs determine plant adaptation and survival, revealing mite infestation has a long-lasting effect negatively impacting seed viability. This study provides additional tools to design pest management strategies that improve resistance without penalty in plant fitness.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Progress in the analysis of phytocannabinoids by HPLC and UPLC (or UHPLC) during 2020-2023},

author = {Lutfun Nahar and Phanuphong Chaiwut and Sarita Sangthong and Tinnakorn Theansungnoen and Satyajit D. Sarker},

url = {10.1002/pca.3374

},

doi = {10.1002/pca.3374},

issn = {0958-0344},

year  = {2024},

date = {2024-07-01},

journal = {PHYTOCHEMICAL ANALYSIS},

volume = {35},

issue = {5},

pages = {927-989},

abstract = {IntroductionOrganic molecules that bind to cannabinoid receptors are known as cannabinoids. These molecules possess pharmacological properties similar to those produced by Cannabis sativa L. High-performance liquid chromatography (HPLC) and ultra-performance liquid chromatography (UPLC, also known as ultra-high-performance liquid chromatography, UHPLC) have become the most widely used analytical tools for detection and quantification of phytocannabinoids in various matrices. HPLC and UPLC (or UHPLC) are usually coupled to an ultraviolet (UV), photodiode array (PDA), or mass spectrometric (MS) detector. ObjectiveTo critically appraise the literature on the application of HPLC and UPLC (or UHPLC) methods for the analysis of phytocannabinoids published from January 2020 to December 2023. MethodologyAn extensive literature search was conducted using Web of Science, PubMed, and Google Scholar and published materials including relevant books. In various combinations, using cannabinoid in all combinations, cannabis, hemp, hashish, C. sativa, marijuana, analysis, HPLC, UHPLC, UPLC, and quantitative, qualitative, and quality control were used as the keywords for the literature search. ResultsSeveral HPLC- and UPLC (or UHPLC)-based methods for the analysis of phytocannabinoids were reported. While simple HPLC-UV or HPLC-PDA-based methods were common, the use of HPLC-MS, HPLC-MS/MS, UPLC (or UHPLC)-PDA, UPLC (or UHPLC)-MS, and UPLC (or UHPLC)-MS/MS was also reported. Applications of mathematical and computational models for optimization of protocols were noted. Pre-analyses included various environmentally friendly extraction protocols. ConclusionDuring the last 4 years, HPLC and UPLC (or UHPLC) remained the main analytical tools for phytocannabinoid analysis in different matrices.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}